Bacterial Overexpression of Recombinant Heteroscorpine-1 (rHS-1), a Toxin from Heterometrus laoticus Scorpion Venom: Trends for Antibacterial Application and Antivenom Production

Uawonggul, Nunthawun; Sukprasert, Sophida; Incamnoi, Paroonkorn; Patramanon, Rina; Thammasirirak, Sompong; Preecharram, Sutthidech; Bunyatratchata, Wandee; Kuaprasert, Buabarn; Daduang, Jureerut; Daduang, Sakda

doi:10.1007/s10528-014-9660-y

Cited by 8 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are many active ingredients in Scorpion, including antimicrobial peptides Mucroporin-M1, 31 analgesic peptide BmKBTx, 32 antiepilepsy peptide AEP, 33 antitumor peptide HS-1, 34 etc. We only detected antitumor effect of Scorpion; however, it remains unclear which active ingredient has the role in inhibiting tumor EMT and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Scorpion inhibits epithelial–mesenchymal transition and metastasis of hepatocellular carcinoma

Yan

Xie

Shi

et al. 2018

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is one of the most malignant diseases worldwide. The unfavorable clinical outcome and poor prognosis are due to high rates of recurrence and metastasis after treatments. Some scholars of traditional Chinese medicine suggested that endogenous wind-evil had played an important role in metastasis of malignant tumor. Therefore, the drug of dispelling wind-evil could be used to prevent cancer metastasis and improve the poor prognosis. So we wondered whether Scorpion, one of the most important wind calming drugs, has antitumor effect especially in epithelial-mesenchymal transition (EMT) and metastasis of HCC in this research. We found that Scorpion-medicated serum could inhibit proliferation, induce apoptosis, and decrease migration and invasion capacity of Hepa1-6 cells in vitro. Meanwhile, we observed that water decoction of Scorpion restrained tumor growth and metastasis in nude mouse of HCC metastasis models. Further experiments showed that Scorpion could suppress EMT, which is characterized by increased epithelial marker E-cadherin expression and decreased mesenchymal markers N-cadherin and Snail expression following Scorpion treatment both in vitro and in vivo. These results suggested that the Scorpion could inhibit Hepa1-6 cells' invasion and metastasis in part by reversing EMT and providing a possible potential approach for preventing HCC metastasis. Impact statement The unfavorable clinical outcome and poor prognosis of hepatocellular carcinoma (HCC) are due to high rates of recurrence and metastasis after treatments. Here we found Scorpion, one of the most important wind calming drugs, has antitumor effect. Scorpion-medicated serum inhibited the proliferation, induced apoptosis, and decreased migration and invasion capacity of Hepa1-6 cells in vitro. Water decoction of Scorpion restrained tumor growth and metastasis in nude mouse of HCC metastasis models. Further experiments showed that Scorpion could suppress EMT of HCC both in vitro and in vivo. Our results suggested that the Scorpion could inhibit Hepa1-6 cells' invasion and metastasis in part by reversing EMT and providing a possible potential approach for preventing HCC metastasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Scorpion inhibits epithelial–mesenchymal transition and metastasis of hepatocellular carcinoma

Yan

Xie

Shi

et al. 2018

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

show abstract

“…Smp76 shares high sequence similarity with two scorpion venom peptides, scorpine and HS-1, that possess antibacterial activities against multiple microorganisms (Fig. 1A) (Carballar-Lejarazu et al 2008;Uawonggul et al 2014). In addition, scorpine exhibits the inhibitory activity to DENV (Carballar-Lejarazu et al 2008).…”

Section: Smp76 Inhibits Denv/zikv Rna Infection and Protein Synthesismentioning

confidence: 98%

“…Its N-terminal amino acid sequence is similar to cecropins, whereas its C-terminal region has three pairs of disulfide bridges, sharing the same CSab motif with defensins (Renaud Conde et al 2000). Sequence similarity showed that Smp76 belongs to the family of b-KTx-like scorpine from Pandinus imperator and HS-1 from Heterometrus laoticus (Renaud Conde et al 2000;Uawonggul et al 2014). Considering the high sequence similarity of Smp76 with scorpine and HS-1 ( Fig.…”

Section: Introductionmentioning

confidence: 99%

The Scorpion Venom Peptide Smp76 Inhibits Viral Infection by Regulating Type-I Interferon Response

Xia

et al. 2018

Virol. Sin.

View full text Add to dashboard Cite

Dengue virus (DENV) and Zika virus (ZIKV) have spread throughout many countries in the developing world and infect millions of people every year, causing severe harm to human health and the economy. Unfortunately, there are few effective vaccines and therapies available against these viruses. Therefore, the discovery of new antiviral agents is critical. Herein, a scorpion venom peptide (Smp76) characterized from Scorpio maurus palmatus was successfully expressed and purified in Escherichia coli BL21(DE3). The recombinant Smp76 (rSmp76) was found to effectively inhibit DENV and ZIKV infections in a dose-dependent manner in both cultured cell lines and primary mouse macrophages. Interestingly, rSmp76 did not inactivate the viral particles directly but suppressed the established viral infection, similar to the effect of interferon (IFN)-b. Mechanistically, rSmp76 was revealed to upregulate the expression of IFN-b by activating interferon regulatory transcription factor 3 (IRF3) phosphorylation, enhancing the type-I IFN response and inhibiting viral infection. This mechanism is significantly different from traditional virucidal antimicrobial peptides (AMPs). Overall, the scorpion venom peptide Smp76 is a potential new antiviral agent with a unique mechanism involving type-I IFN responses, demonstrating that natural AMPs can enhance immunity by functioning as immunomodulators. Keywords Dengue virus (DENV) Á Zika virus (ZIKV) Á Scorpion venom peptide Á Smp76 Á Antiviral mechanism Á Type-I interferon response Electronic supplementary material The online version of this article (

show abstract

“…Similarly, the venom of Vaejovis mexicanus (Koch, 1836) was more effective against Gram-negative bacteria, such as P. aeruginosa including multidrug-resistant strains [28]. Certain species, such as Heterometrus laocticus, Tityus discrepans and Opistophthalmus carinatus, produce peptides with antimicrobial effects called defensins [64][65][66]; other related peptides, such as Opistoporin-1 (Opistophthalmus carinatus) and Pandinin-1 (Pandinus imperator), both with 44 amino acids, have antimicrobial activity against Gram-positive and Gram-negative bacteria, respectively [21,67].…”

Section: Peptides With Antibacterial Activity (Abps)mentioning

confidence: 99%

Antimicrobial Activity Developed by Scorpion Venoms and Its Peptide Component

Rincón-Cortés

Bayona-Rojas

Reyes-Montaño

et al. 2022

Toxins

View full text Add to dashboard Cite

Microbial infections represent a problem of great importance at the public health level, with a high rate of morbidity-mortality worldwide. However, treating the different diseases generated by microorganisms requires a gradual increase in acquired resistance when applying or using them against various antibiotic therapies. Resistance is caused by various molecular mechanisms of microorganisms, thus reducing their effectiveness. Consequently, there is a need to search for new opportunities through natural sources with antimicrobial activity. One alternative is using peptides present in different scorpion venoms, specifically from the Buthidae family. Different peptides with biological activity in microorganisms have been characterized as preventing their growth or inhibiting their replication. Therefore, they represent an alternative to be used in the design and development of new-generation antimicrobial drugs in different types of microorganisms, such as bacteria, fungi, viruses, and parasites. Essential aspects for its disclosure, as shown in this review, are the studies carried out on different types of peptides in scorpion venoms with activity against pathogenic microorganisms, highlighting their high therapeutic potential.

show abstract

Bacterial Overexpression of Recombinant Heteroscorpine-1 (rHS-1), a Toxin from Heterometrus laoticus Scorpion Venom: Trends for Antibacterial Application and Antivenom Production

Cited by 8 publications

References 24 publications

Scorpion inhibits epithelial–mesenchymal transition and metastasis of hepatocellular carcinoma

Scorpion inhibits epithelial–mesenchymal transition and metastasis of hepatocellular carcinoma

The Scorpion Venom Peptide Smp76 Inhibits Viral Infection by Regulating Type-I Interferon Response

Antimicrobial Activity Developed by Scorpion Venoms and Its Peptide Component

Contact Info

Product

Resources

About