Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based

Cai, Wei; Kesavan, Dinesh Kumar; Wan, Jie; Abdelaziz, Mohamed Hamed; Su, Zhaoliang; Xu, Huaxi

doi:10.1186/s13000-018-0768-y

Cited by 44 publications

(51 citation statements)

References 118 publications

(131 reference statements)

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“…Owing to their adaptability as a delivery vehicle, the contributions of OMVs to bacterial fitness are diverse, but there is increasing interest on their role in host colonization and disease pathogenesis ( 9 , 10 ). These features and the intrinsic adjuvant capacity render OMVs as promising candidates for vaccine development against bacterial infections ( 11 , 12 ). Despite their prophylactic potential there are few studies exploring the direct effects of OMVs on monocytes/macrophages and inflammasome activation.…”

Section: Introductionmentioning

confidence: 99%

Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis

et al. 2020

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Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived from Bordetella pertussis (BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfected B. pertussis lipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination.

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Section: Introductionmentioning

confidence: 99%

Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis

et al. 2020

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Section: Introductionmentioning

confidence: 99%

“…Because of the amount of antigen present in membrane blebs and its ability to modulate the immune response, they have been tested as acellular vaccines candidates (Cai et al, 2018). Membrane blebs from different pathogens such as Escherichia coli, Vibrio cholerae or Neisseria meningitidis have been used to immunize animals and then challenge them with the progenitor pathogenic bacterium to assess the efficacy of the protective immune response (Yu et al, 2017; Cai et al, 2018). One of the characteristics that make membrane blebs attractive to develop vaccines is that these contain natural components of the bacterial cells, so they may induce an immune response in vitro and in vivo but without generating infection in the host, because blebs are considering acellular entities.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Membrane Blebs of Brucella abortus 2308 and RB51 and Their Evaluation as an Acellular Vaccine

et al. 2019

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Membrane blebs are released from Gram-negative bacteria, however, little is known about Brucella blebs. This work pursued two objectives, the first was to determine and identify the proteins in the membrane blebs by proteomics and in silico analysis. The second aim was to evaluate the use of membrane blebs of Brucella abortus 2308 and B. abortus RB51 as an acellular vaccine in vivo and in vitro. To achieve these aims, membrane blebs from B. abortus 2308 and RB51 were obtained and then analyzed by liquid chromatography coupled to mass spectrometry. Brucella membrane blebs were used as a “vaccine” to induce an immune response in BALB/c mice, using the strain B. abortus RB51 as a positive vaccine control. After subsequent challenge with B. abortus 2308, CFUs in spleens were determined; and immunoglobulins IgG1 and IgG2a were measured in murine serum by ELISA. Also, activation and costimulatory molecules induced by membrane blebs were analyzed in splenocytes by flow cytometry. Two hundred and twenty eight proteins were identified in 2308 membrane blebs and 171 in RB51 blebs, some of them are well-known Brucella immunogens such as SodC, Omp2b, Omp2a, Omp10, Omp16, and Omp19. Mice immunized with membrane blebs from rough or smooth B. abortus induced similar protective immune responses as well as the vaccine B. abortus RB51 after the challenge with virulent strain B. abortus 2308 (P < 0.05). The levels of IgG2a in mice vaccinated with 2308 membrane blebs were higher than those vaccinated with RB51 membrane blebs or B. abortus RB51 post-boosting. Moreover, mice immunized with 2308 blebs increased the percentage of activated B cells (CD19+CD69+) in vitro. Therefore, membrane blebs are potential candidates for the development of an acellular vaccine against brucellosis, especially those derived from the rough strains so that serological diagnostic is not affected.

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“…In vivo , OMVs have a wide variety of interactions with immune cells showing their potential to be used for immunization ( Kaparakis-Liaskos and Ferrero, 2015 ; Cai et al, 2018 ). The first studies into immune responses evoked by OMVs already showed promising inductions of cytokines and chemokines in macrophages and other cell types.…”

Section: Omvs In Vaccinationmentioning

confidence: 99%

Outer Membrane Vesicle Induction and Isolation for Vaccine Development

2021

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Gram-negative bacteria release vesicular structures from their outer membrane, so called outer membrane vesicles (OMVs). OMVs have a variety of functions such as waste disposal, communication, and antigen or toxin delivery. These vesicles are the promising structures for vaccine development since OMVs carry many surface antigens that are identical to the bacterial surface. However, isolation is often difficult and results in low yields. Several methods to enhance OMV yield exist, but these do affect the resulting OMVs. In this review, our current knowledge about OMVs will be presented. Different methods to induce OMVs will be reviewed and their advantages and disadvantages will be discussed. The effects of the induction and isolation methods used in several immunological studies on OMVs will be compared. Finally, the challenges for OMV-based vaccine development will be examined and one example of a successful OMV-based vaccine will be presented.

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Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based

Cited by 44 publications

References 118 publications

Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis

Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis

Proteomic Analysis of Membrane Blebs of Brucella abortus 2308 and RB51 and Their Evaluation as an Acellular Vaccine

Outer Membrane Vesicle Induction and Isolation for Vaccine Development

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