Bacterial nanotubes mediate bacterial growth on periodic nano-pillars

Cao, Yunyi; Jana, Saikat; Bowen, Leon; Li, Hongzhong; Jakubovics, Nicholas S.; Chen, Jinju

doi:10.1039/d0sm00602e

Cited by 7 publications

(8 citation statements)

References 54 publications

(97 reference statements)

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“…These nanotubes were found to connect two different bacterial cells and are involved in the transfer of cytoplasmic material between bacterial cells of the same and different species, and even to eukaryotic cells [18][19][20][21][22][23][24]. In addition, a recent study suggested that nanotubes assist the growth of Pseudomonas aeruginosa on periodic nano-pillar surfaces [25].…”

Section: Introductionmentioning

confidence: 99%

“…These nanotubes were found to connect two different bacterial cells and are involved in the transfer of cytoplasmic material between bacterial cells of the same and different species, and even to eukaryotic cells ( Bhattacharya et al, 2019 ; Dubey and Ben-Yehuda, 2011 ; Baidya et al, 2018 ; Pande et al, 2015 ; Benomar et al, 2015 ; Baidya et al, 2020 ; Pal et al, 2019 ). In addition, a recent study suggested that nanotubes assist the growth of Pseudomonas aeruginosa on periodic nano-pillar surfaces ( Cao et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

Kaplan

Chreifi

Metskas

et al. 2021

eLife

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The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~ 90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: 1) tubes with a uniform diameter (with or without an internal scaffold), 2) tubes with irregular diameter, 3) tubes with a vesicular dilation at their tip, 4) pearling tubes, 5) connected chains of vesicles (with or without neck-like connectors), 6) budding vesicles and nanopods. We also identified several protein complexes associated with these MEs and MVs which were distributed either randomly or exclusively at the tip. These complexes include a secretin-like structure and a novel crown-shaped structure observed primarily in vesicles from lysed cells. In total, this work helps to characterize the diversity of bacterial membrane projections and lays the groundwork for future research in this field.

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Section: Introductionmentioning

confidence: 99%

“…These nanotubes were found to connect two different bacterial cells and are involved in the transfer of cytoplasmic material between bacterial cells of the same and different species, and even to eukaryotic cells ( Bhattacharya et al, 2019 ; Dubey and Ben-Yehuda, 2011 ; Baidya et al, 2018 ; Pande et al, 2015 ; Benomar et al, 2015 ; Baidya et al, 2020 ; Pal et al, 2019 ). In addition, a recent study suggested that nanotubes assist the growth of Pseudomonas aeruginosa on periodic nano-pillar surfaces ( Cao et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

Kaplan

Chreifi

Metskas

et al. 2021

eLife

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“…It has been hypothesized that these nanotubes enable bacteria scavenge and deliver molecules inside them and represent an important form of bacterial communication in nature, providing a network for exchange of cellular molecules within and between species [25]. It was found that nanotubes mediate the transfer of cytoplasmic molecules between adjacent cells [26] , [27]. Moreover, they may also enable transiently acquired nonhereditary resistance to antibiotics.…”

Section: Ftir-atr Results Presented In Figure S3 Complement This Information (See Si For Details)mentioning

confidence: 99%

Different Impact of Suspended Al2O3 Nanoparticles on Microbial Communities: Formation of 2D-Networks (Without Humic Acids) or 3D-Colonies (With Humic Acids)

et al. 2022

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The use of metal-based and, particularly, Al 2 O 3 nanoparticles (Al 2 O 3− NP) for diverse purposes is exponentially growing. However, the growth of such promissory market is not accompanied by a parallel extensive investigation related to the impact of this pollution on groundwater and biological systems. Pseudomonas species, ubiquitous, environmentally critical microbes, frequently respond to stress conditions with diverse strategies that generally include extracellular polymeric substances (EPS) formation. The aim of this study is to report that changes in the aqueous environment, particularly, the addition of Al 2 O 3 -NP without and with humic acids, induce different adaptive strategies of P. aeruginosa early bio lms. To this purpose, early bio lms were incubated in diluted culture media without (control) and with Al 2 O 3 -NP, and with humic acids (HA-control, HA-Al 2 O 3 -NP) for 24h. 3D colonies with EPS strings and isolated bacteria in their surroundings were detected in the control bio lms. Unlikely, an unusual adaptive behaviour was developed in presence of Al 2 O 3 -NP. Bacteria opt to disassemble the 3D arrangements, and to implement a 2D network promoting morphological and size changes of bacterial cells (small coccoid shapes). Remarkably, this strategy allows their temporarily non-EPS-depending survival without decreasing the number of cells. This behaviour was not observed with ZnO-NP, HA-Al 2 O 3 -NP, or HA-ZnO-NP. Physicochemical analysis revealed that HA were adsorbed on Al 2 O 3 -NP and promoted the Al(III) ions complexation. This supports the hypothesis that the reduction of toxicity of Al ions and the 3D colony formation in presence of HA-Al 2 O 3 -NP is promoted by the complexation of the metal ions with HA components.

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“…Apart from molecular trading, nanotubes are perceived as an anchoring factor required for cell-surface and cell-cell attachment. 54 In nutrient-deprived conditions, nanotube formation is induced among bacteria to facilitate the movement of nutrients to the intended bacteria. 21 Moreover, auxotrophs acquire essential amino acids from the donor cells through nanotubes, which results in the restriction of feedback inhibition of the respective amino acid biosynthetic pathway.…”

Section: Nanotubes-mediated Interactionsmentioning

confidence: 99%

Molecular trafficking between bacteria determines the shape of gut microbial community

2021

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Complex inter-bacterial interactions largely influence the structure and function of the gut microbial community. Though several host-associated phenomena have often been shown to be involved in the stability, structure, and function of the gut microbial community, the implication of contact-dependent and contact-independent inter-bacterial interactions has been overlooked. Such interactions are tightly governed at multiple layers through several extracellular organelles, including contact-dependent inhibition (CDI), nanotubes, type VI secretion system (T6SS), and membrane vesicles (MVs). Recent advancements in molecular techniques have revealed that such extracellular organelles function beyond exhibiting competitive behavior and are also involved in manifesting cooperative behaviors. Cooperation between bacteria occurs through the sharing of several beneficial molecules including nucleic acids, proteins, metabolites, and nutrients among the members of the community, while competition occurs by means of multiple toxins. Intrinsic coordination between contact-dependent and contact-independent mechanisms collectively provides a fitness advantage and increased colonization resistance to the gut microbiota, where molecular trafficking plays a key role. This review is intended to provide a comprehensive view of the salient features of the different bacterial interactions and to highlight how microbiota deploy multifaceted organelles, for exerting both cooperative and competitive behaviors. We discuss the current knowledge of bacterial molecular trafficking and its impact on shaping the gut microbial community.

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Bacterial nanotubes mediate bacterial growth on periodic nano-pillars

Abstract: Surface topography designed to achieve spatial segregation has shown promise in delaying bacterial attachment and biofilm growth. However, the underlying mechanisms linking surface topography to the inhibition of microbial attachment...

Cited by 7 publications

References 54 publications

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

Different Impact of Suspended Al2O3 Nanoparticles on Microbial Communities: Formation of 2D-Networks (Without Humic Acids) or 3D-Colonies (With Humic Acids)

Molecular trafficking between bacteria determines the shape of gut microbial community

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