Bacterial Microflora of Contaminated Metalworking Fluids

Bakalova, Snezhana M.; Doycheva, A.; Ivanova, I.; Groudeva, V.; Dimkov, R.

doi:10.1080/13102818.2007.10817490

Cited by 24 publications

(21 citation statements)

References 10 publications

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“…The bacterial species abundance observed in this study was similar to that reported by others (Bakalova et al 2007;Gilbert et al 2010). Pseudomonas spp.…”

Section: Discussionsupporting

confidence: 91%

Microbial contamination and biofilms on machines of metal industry using metalworking fluids with or without biocides

Trafny

Lewandowski

Kozłowska

et al. 2015

International Biodeterioration & Biodegradation

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“…The bacterial species abundance observed in this study was similar to that reported by others (Bakalova et al 2007;Gilbert et al 2010). Pseudomonas spp.…”

Section: Discussionsupporting

confidence: 91%

Microbial contamination and biofilms on machines of metal industry using metalworking fluids with or without biocides

Trafny

Lewandowski

Kozłowska

et al. 2015

International Biodeterioration & Biodegradation

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“…John and Phillips (2002) demonstrated that MWF spiked with P. fluorescens (10 7 CFU/ml) and irradiated with a 6-W submerged non-glass UV lamp resulted in two-log reduction in 60 mins. Peppiatt and Shama (2000) irradiated microbially contaminated commercial MWF using a thin film Microbiological contaminants of metalworking fluids in service Baecker et al (1989) Bacterial microflora of contaminated metalworking fluids Bakalova et al (2007) Microbiology of metalworking fluids: pilot studies of a large-scale exposure assessment experience Burge (1996) The survival of Legionella pneumophilia in dilute metalworking fluids Elsmore (2003) Metalworking fluids: oil mist and beyond Gauthier (2003) Metalworking fluids biodiversity characterization Gilbert et al (2010a, b) Investigation into the nature and extent of microbial contamination present in a commercial metalworking fluid Lin et al (1999) Occurrence and characterization of culturable bacteria and fungi in metalworking environments Liu et al (2010) Evaluation Virji et al (2000) contractor at a flow rate of 1.8×10 −2 m 3 s −1 at a UV dose of 44.5 mW s/cm 3 . The MWF was irradiated in the form of "bells" generated with a specially designed nozzle which finally led to a decline of 10 6 to 10 7 CFU/ml that occurred within 6-8 h. Similar works carried out for endotoxin inactivation of drinking water by UV irradiation suggested that 1 to 50 endotoxin units (EU/ml) could effectively be inactivated with UV fluences of up to 500 mJ/cm 2 (Anderson et al 2003).…”

Section: Ultraviolet Irradiationmentioning

confidence: 99%

“…High levels of contamination ranging from 10 4 to 10 10 CFU/ml have been reported in MWFs (Mattsby-Blatzer et al 1989;Sloyer et al 2002;van der Gast et al 2003). A wide variety of microorganisms such as Staphylococcus, Streptococcus, Pseudomonas, Alcaligenes, sulfate-reducing bacteria (SRB), and Acinetobacter are known to inhabit MWFs (Mattsby-Blatzer et al 1989;Perkins and Angenent 2010;Sandin et al 1991;Virji et al 2000;van der Gast et al 2003) ( Table 2), including pathogens (opportunistic) such as Legionella sp., Klebsiella pneumonia, Pseudomonas aeruginosa, and Escherichia coli (Bakalova et al 2007;Elsmore 2003;Lucchesi et al 2012). Relatively recently, a new species of Mycobacterium (Mycobacterium immunogenum) was isolated from used MWFs that was associated with hypersensitivity pneumonitis (Moore et al 2000;Wallace et al 2002;Rhodes et al 2011).…”

Section: Introductionmentioning

confidence: 99%

The microbiology of metalworking fluids

Saha

Donofrio

2012

Appl Microbiol Biotechnol

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Metalworking fluids (MWFs) are complex mixtures of chemicals and are indispensable materials in industry. They are used as cooling and lubricating agents in different machining process such as grinding, milling, and cutting. The quality of MWFs is affected by physical, chemical, and microbial contaminates. In particular, MWFs are highly vulnerable to microbial contamination, which may act both as potential pathogens and deteriorgens. Microbial contamination is of major concern due to potential health hazards such as skin dermatitis and hypersensitivity pneumonitis. The contaminated MWFs can exhibit high degrees of microbial loading, ranging from 10(4) to 10(10) colony-forming units (CFU)/ml. Wide varieties of microorganisms are reported to colonize MWFs. Traditional culturing techniques are not only laborious and time consuming but also underestimate the actual distribution of the microorganisms present in the contaminated MWFs. Therefore, rapid molecular methods such as real-time PCR and fluorescent in situ hybridization are implemented to monitor the microbial load. In industry, biocides are presently used to control microbial contamination. However, it has its own disadvantages and therefore, in recent years, alternative methods such as UV irradiation were evaluated to reduce microbial contamination in MWFs. Microbes inhabiting the MWF are also capable of forming biofilm which is detrimental to the MWF system. Biofilm is resistant to common disinfectant methods, and thus further research and development is required to effectively control its formation within MWF systems. This review is intended to discuss the overall microbiological aspects of MWF.

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“…The first strategy involves either biostimulation of the indigenous microflora within the treated MWF [9,39] or selection of the most active strains from various MWF samples, then cultivation and inoculation to a new wastewater to be treated [9,30,34,35,[40][41][42]. The use of indigenous microorganisms is of a special interest since their resistance to a complex chemical content of MWFs might result in elevated xenobiotic biodegradation activity [13,14,40,43].…”

Section: Introductionmentioning

confidence: 99%

“…Apart from oil-derived xenobiotics they contain a number of additives including biocides, heavy metals, surfactants and many other agents [3,11]. In addition, cutting fluids are often contaminated by various bacterial (both gram-negative and gram-positive) and fungal strains (for detailed information see the review articles [12][13][14][15][16]). Such indigenous microbial strains may be beneficial for their tolerance towards toxic chemical constituents; however, they are often pathogenic and can cause adverse effects on human health, especially on the respiratory system and skin [3,[17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Kaszycki

Petryszak

Supel

2015

Ecological Chemistry and Engineering S

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Spent mineral oil-based metalworking fluids are waste products of the machining processes and contribute substantially to the global industrial pollution with petroleum oil products. Wastewaters containing oily emulsions are ecologically hazardous and thus a variety of methods have been implemented to prevent these effluents from affecting the natural environment. Most of these methods rely upon physical-chemical treatment and phase separation; however, none of them proved to be effective enough to meet tightening environmental regulations. Therefore, novel technologies need to be elaborated and there is growing interest in implementing biological treatment methods based on microbial bioremediation. In this study an oil/water emulsion obtained from a waste stream of the metal-processing industry was tested for biodegradability of its organic constituents. This liquid waste was found non-toxic to bacterial consortia and was colonized with indigenous microorganisms (approx. 10 7 cfu · cm -3). The total load of organic content was determined as a chemical oxygen demand (COD) value of 48 200 mg O2 · dm -3. Emulsion treatment was carried out using a threefold wastewater dilution and employing two variants of biostimulated aerobic bacterial communities: (1) uninoculated emulsion, where bioremediation was carried out by the autochthonous bacteria alone, and (2) wastewater samples inoculated with a ZB-01 microbial consortium which served as a source of specialized bacteria for process bioaugmentation. Biodegradation efficiency achieved in a 14-day test was monitored by measuring both the COD parameter and the concentration of high-boiling organic compounds. Both approaches yielded satisfactory results showing significant reduction of the emulsion organic fraction; however, the resultant decrease of wastewater load tended to be more efficient for the case where the process was bioaugmented with the inoculated consortium. Gas chromatography analyses coupled with mass spectrometric detection (GC-MS) confirmed high degradation yields obtained for both cases studied (58 and 71%, respectively) in a 28-day test. It is concluded that oil-based metalworking emulsions can undergo efficient biological treatment under conditions enabling aerobic bacterial proliferation and that xenobiotic biodegradation kinetics can be accelerated by bioaugmenting the process with allochthonous microbial consortia.

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Bacterial Microflora of Contaminated Metalworking Fluids

Cited by 24 publications

References 10 publications

Microbial contamination and biofilms on machines of metal industry using metalworking fluids with or without biocides

Microbial contamination and biofilms on machines of metal industry using metalworking fluids with or without biocides

The microbiology of metalworking fluids

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

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