Bacterial metabolite mediated differential human PMN gene expression

Swartwout, S G; Niederman, Richard

doi:10.1111/j.1600-0765.1997.tb01405.x

Cited by 8 publications

(8 citation statements)

References 24 publications

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“…Using dierential display analysis (DDA), Swartwout et al recently reported that a number of dierentially appearing bands were identi®ed in human neutrophils stimulated with bacterial metabolites, short chain carboxylic acids (45). They also showed that stimulated neutrophils could have an ability to express a number of transcripts.…”

Section: Discussionmentioning

confidence: 99%

Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis

Morozumi

Kubota

Sugita

et al. 2001

J of Periodontal Research

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Differential gene expression was examined in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis (P. gingivalis-LPS) using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR). LPS from Escherichia coli (E. coli-LPS) was used as control. More than 200 differently expressed transcripts were found in 8 subjects showing differential regulation at the transcriptional level. Densitometric analyses revealed that 42-100 genes were upregulated, while 53-116 genes were downregulated by P. gingivalis-LPS compared with E. coli-LPS. The results of sequencing identification showed the presence of supervillin (SVIL) and vascular endothelial growth factor (VEGF) genes in the clones which were upregulated by P. gingivalis-LPS. Consequently, semiquantitative analyses proved that the level of mRNA for SVIL and VEGF were significantly higher in P. gingivalis-LPS-stimulated neutrophils than in other bacterial (E. coli, Actinobacillus actinomycetemcomitans, Prevotella intermedia) LPS- or synthetic lipid A-stimulated neutrophils. Our findings suggest that an elevated mRNA expression for SVIL could be associated with impaired function of neutrophils when stimulated by P. gingivalis-LPS. Further, the VEGF mRNA over-expression might be related to the pathogenesis of P. gingivalis-associated periodontitis. The RAP-PCR technique used in this study enabled us to identify a number of P. gingivalis-LPS regulated genes which hitherto have not been reported.

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Section: Discussionmentioning

confidence: 99%

Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis

Morozumi

Kubota

Sugita

et al. 2001

J of Periodontal Research

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“…Recently, we have reported that a number of dierentially displayed bands were obtained from human neutrophils stimulated with LPS from P. gingivalis (6). Other researchers have also shown that stimulated neutrophils have an ability to express a number of transcripts (1,26). To assess dierences in susceptibility of neutrophils between the dierent patient groups and healthy subjects, we have utilized a single dose of FMLP for RAP-PCR analyses.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in neutrophils from patients with generalized aggressive periodontitis

Kubota

Morozumi

Shimizu

et al. 2001

J of Periodontal Research

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Differential gene expression was investigated in neutrophils stimulated with N-formyl-methionyl-leucyl-phenylalanine using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR). The cells were isolated from 3 groups of subjects: patients with generalized aggressive periodontitis (Aggressive-P. n = 6), generalized chronic periodontitis (Chronic-P, n = 6) and healthy controls (H, n = 8). Our results show that 37 genes were upregulated. while 27 genes were down-regulated in all Aggressive-P neutrophils by using RAP-PCR with 45 primer pairs. Reverse transcription-PCR analyses revealed that mRNA levels were significantly different (p<0.05) for heat shock transcription factor 4b (HSF4b) gene. Kruppel-like zinc finger transcription factor 9 (Zf9) and muskelin genes. HSF4b was greater in neutrophils from Aggressive-P compared to groups H and Chronic-P. Zf9 and muskelin genes were lower in Aggressive-P compared to the H groups, but no significant difference was noted compared to the Chronic-P group. The control genes, IL-1beta and VEGF genes, were expressed at a significantly higher level in Aggressive-P and Chronic-P than H (p<.01, p<0.05). In conclusion, the RAP-PCR technique used in this study enabled us to identify 3 Aggressive-P related genes, which had not been reported previously. Neutrophil functions in Aggressive-P patients are suggested to be altered by regulatory factors of the immune system including HSF4b (transcription factor), Zf9 (activator of TGF-beta) and muskelin (cellular adhesion).

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“…For example, Botta et al (1985), Courtois et al (1989), and Niederman et al (1995) showed that the levels of SCCA in the plaque and GCF of patients diagnosed as having periodontal diseases were significantly higher than those found in plaque and GCF from healthy subjects. SCCA can inhibit neutrophil function (Kyner et al, 1976;Touw et al, 1982;Rotstein et al, 1987Rotstein et al, , 1988Eftimiadi et al, 1993), increase gene and protein expression (Stringer et al, 1996;Swartout and Niederman, 1997), increase cytokine gene expression (Bingel et al, 1987;Eftimiadi et al, 1991;KuritaOchiai et al, 1995;Zhang, 1996), and delay apoptosis (Stringer et al, 1996). The subject was reviewed recently by Niederman et al (1997).…”

Section: Introductionmentioning

confidence: 93%

Gingival Inflammation Induced by Food and Short-chain Carboxylic Acids

Kashket¹,

Zhang²,

Niederman³

1998

J Dent Res

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Earlier studies in our laboratories demonstrated that particles of a number of snack foods that are retained on the dentition accumulate fermentable sugars and short-chain carboxylic acids (SCCA; acetic, formic, lactic, and propionic) to different degrees. The present study was undertaken to test the hypothesis that the accumulated SCCA can induce a gingival inflammatory response. Five periodontally and medically healthy subjects were given portions of plain doughnuts (high SCCA levels) or oatmeal cookie (low SCCA), or had the SCCA applied directly to the gingival margins of designated teeth. Subjects were given wax to chew, or nothing, as controls. Inflammation was assessed by measurements of subgingival temperature, flow rates of gingival crevicular fluid (GCF), and neutrophil emigration into GCF. Subgingival temperatures of the maxillary gingiva rose by 1.32 +/- 0.30 degrees C (mean +/- SE) 5 min after the subjects consumed the doughnuts and remained elevated for at least 1 hr. These values were significantly higher than those obtained from subjects after ingestion of oatmeal cookies (0.63 +/- 0.17 degree C; p < 0.01), consistent with the low levels of SCCA in the retained cookie particles. Wax chewing elicited a similar response, indicating a masticatory effect on the gingiva. Gingival temperatures in the unchallenged controls remained unchanged. Neutrophil emigration into the GCF was significantly elevated in subjects after doughnut consumption. Rinses with a solution of SCCA, or application of the SCCA to the gingiva, also brought about significant elevations in subgingival temperature and neutrophil emigration. The findings describe the inflammatory effects of food ingestion on the gingiva of healthy human subjects, and support the hypothesis that SCCA in the particles of retained food are at least partly responsible for the observed responses.

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Bacterial metabolite mediated differential human PMN gene expression

Cited by 8 publications

References 24 publications

Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis

Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis

Differential gene expression in neutrophils from patients with generalized aggressive periodontitis

Gingival Inflammation Induced by Food and Short-chain Carboxylic Acids

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