Bacterial membrane vesicles as promising vaccine candidates

Jiang, Linglei; Schinkel, Michelle; Essen, Max van; Schiffelers, Raymond M.

doi:10.1016/j.ejpb.2019.09.021

Cited by 31 publications

(33 citation statements)

References 55 publications

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“…On the other hand, compared with nanoparticles based biomimic‐ECM, the native origins of EVs whose surface has inherent biochemical properties, results in a low immune response, especially if they are derived from appropriate cells (including MSCs). [ 68 ] Furthermore, EVs were easier to be engineered and modified compared to cells, [ 69 ] further studies to improve the biological functions and mitigate the potential risks of EVs from allogenic donors should be developed in the future for better applications in allotransplantation.…”

Section: Resultsmentioning

confidence: 99%

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Wei

Shi

Zhang

et al. 2020

Adv Funct Materials

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Restoring extracellular matrix (ECM) with supportive and osteoinductive abilities is of great significance for bone tissue regeneration. Current approaches involving cell-based scaffolds or nanoparticle-modified biomimic-ECM have been met with additional biosafety concerns. Herein, the natural biomineralization process is first analyzed and is found that mesenchymal stem cells-derived extracellular vesicles (EVs) from early and late stages of osteoinduction play different roles during the mineralization process. The functional EVs hierarchically with blood-derived autohydrogel (AH) are then incorporated to form an osteoinductive biomimetic extracellular matrix (BECM). The alkaline phosphatase-rich EVs are released from the outer layers to induce osteoblast differentiation during early stages. Thereafter, as the degradation of AH occurred, calcium/phosphorus (Ca/P)rich EVs are liberated to promote the nucleation of extracellular mineral crystals. Additionally, BECM contains considerable collagen fibrils that provide additional nucleation sites for crystallites deposition, thus reaching self-mineralization in situ. In conclusion, this research provides a promising, versatile mineralization-instructive platform to tackle the challenges faced in bone-tissue engineering. Scheme 1. Illustration of the BECM induced osteogenesis and biomineralization. In the early stage of mineralization, BECM secreted ALP-rich E-EVs. The E-EVs moved to intracellular mitochondria and provided ALP for phosphate metabolism, contributing to the formation of Ca/P-rich vesicles. Later, with the degradation of BECM, Ca/P-rich L-EVs were released gradually. The L-EVs aligned to extracellular collagen and promoted the nucleation of extracellular mineral crystals. Besides, L-EVs inside BECM aggregate around the internally collagen fibrils and initiated in situ mineralization. Finally, with the complete degradation of BECM, the internal mineralized components were released to the extracellular matrix, promoting the deposition formation together to realize an ideal self-mineralization process for bone tissue regeneration.www.afm-journal.de www.advancedsciencenews.com (3 of 15)

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Section: Resultsmentioning

confidence: 99%

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Wei

Shi

Zhang

et al. 2020

Adv Funct Materials

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“…These features make EVs good candidates for vaccine development. Several studies have shown that EVs can induce adaptive immunity and confer protection against infections caused by both Gram-negative and Grampositive pathogenic bacteria [83][84][85]. For instance, mice immunized with 1 μg of E. coli derived OMVs resulted in 100% protection against a lethal dose challenge, while the survival rate was only 20% in the untreated group [86].…”

Section: Use Of Evs As a Vaccine Platformmentioning

confidence: 99%

Extracellular Vesicles and Their Role in Staphylococcus aureus Resistance and Virulence

Luz¹,

Azevedo²,

Loir³

et al. 2021

Infectious Diseases

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Staphylococcus aureus is a pathogen of great importance to clinical and veterinary medicine. Recently, there has been a growing interest in S. aureus extracellular vesicles (EVs) in the pathogenesis of this bacterium. Released by living cells into the extracellular milieu, EVs are membranous structures carrying macromolecules such as proteins, nucleic acids, and metabolites. These structures play several physiological roles and are, among others, considered a mechanism of intercellular communication within S. aureus populations but also in trans kingdom interactions. S. aureus EVs were shown to transport important bacterial survival and virulence factors, such as β-lactamases, toxins, and proteins associated with bacterial adherence to host cells, and to trigger the production of cytokines and promote tissue inflammation. In this chapter, we will review the main studies regarding S. aureus EVs, including their composition and roles in host-pathogen interactions, and the possible applications of EVs for vaccines and therapy development against staphylococcal infections.

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“…Immune responses for DODAB BF and alum complexes with outer membrane vesicles (OMV) of Neisseria meningitidis B administered by intranasal and subcutaneous routes in mice were compared; intranasal immunization produced a mixed Th1 and Th2 response, while subcutaneous immunization exhibited a Th1 profile only [ 27 ]. Non-replicating, nanometric membrane vesicles (MV) released both by Gram-positive and Gram-negative bacteria contain proteins, lipids, and nucleic acids that are effectively able to stimulate the innate and adaptive immune system [ 85 , 86 ]. In this regard, the cationic lipids can add extra adjuvanticity.…”

Section: Assemblies From Cationic Lipids and Surfactantsmentioning

confidence: 99%

Cationic Nanostructures for Vaccines Design

Carmona-Ribeiro

Betancourt

2020

Biomimetics

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Subunit vaccines rely on adjuvants carrying one or a few molecular antigens from the pathogen in order to guarantee an improved immune response. However, to be effective, the vaccine formulation usually consists of several components: an antigen carrier, the antigen, a stimulator of cellular immunity such as a Toll-like Receptors (TLRs) ligand, and a stimulator of humoral response such as an inflammasome activator. Most antigens are negatively charged and combine well with oppositely charged adjuvants. This explains the paramount importance of studying a variety of cationic supramolecular assemblies aiming at the optimal activity in vivo associated with adjuvant simplicity, positive charge, nanometric size, and colloidal stability. In this review, we discuss the use of several antigen/adjuvant cationic combinations. The discussion involves antigen assembled to 1) cationic lipids, 2) cationic polymers, 3) cationic lipid/polymer nanostructures, and 4) cationic polymer/biocompatible polymer nanostructures. Some of these cationic assemblies revealed good yet poorly explored perspectives as general adjuvants for vaccine design.

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Bacterial membrane vesicles as promising vaccine candidates

Cited by 31 publications

References 55 publications

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Autologous Versatile Vesicles‐Incorporated Biomimetic Extracellular Matrix Induces Biomineralization

Extracellular Vesicles and Their Role in Staphylococcus aureus Resistance and Virulence

Cationic Nanostructures for Vaccines Design

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