Bacterial Lipopolysaccharide Plus Interferon-Ɣ Elicit a Very Fast Inhibition of a Ca2+-dependent Nitric-oxide Synthase Activity in Human Astrocytoma Cells

Colasanti, Marco; Cavalieri, Elisabetta; Persichini, Tiziana; Mollace, Vincenzo; Mariotto, Sofia; Suzuki, Hisanori; Lauro, Giuliana M.

doi:10.1074/jbc.272.12.7582

Cited by 41 publications

(30 citation statements)

References 27 publications

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“…As a consequence, iNOS gene expression takes place after LPS/cytokine stimulation, provided that the cNOS-generated NO is reduced below a threshold value in a short time [15,17]. In this respect, we have recently reported that iNOS inducers (e.g., LPS and IFNc) elicit a rapid inactivation of nNOS and a decrease of basal NO levels [24], an event mediated by arachidonic acid-dependent tyrosine phosphorylation of nNOS [15,25].…”

Section: Resultsmentioning

confidence: 99%

Nitric oxide mediates anti‐inflammatory action of extracorporeal shock waves

et al. 2005

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Here, we show that extracorporeal shock waves (ESW), at a low energy density value, quickly increase neuronal nitric oxide synthase (nNOS) activity and basal nitric oxide (NO) production in the rat glioma cell line C6. In addition, the treatment of C6 cells with ESW reverts the decrease of nNOS activity and NO production induced by a mixture of lipopolysaccharides (LPS), interferon-c (IFN-c) plus tumour necrosis factor-a (TNF-a). Finally, ESW treatment efficiently downregulates NF-jB activation and NF-jB-dependent gene expression, including inducible NOS and TNF-a. The present report suggests a possible molecular mechanism of the anti-inflammatory action of ESW treatment.

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Section: Resultsmentioning

confidence: 99%

Nitric oxide mediates anti‐inflammatory action of extracorporeal shock waves

et al. 2005

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“…As described previously, homogenates of unstimulated T67 cells exhibited a basal NOS-I activity (8). The elimination of free Ca 2ϩ by EGTA (1 mM) from the reaction mixture almost totally abolished this activity (Fig.…”

Section: Tyrosine Phosphorylation Inactivates Nos-i 9916mentioning

confidence: 99%

“…Ca 2ϩ -dependent NOS activity but not NOS-I mRNA expression (8). Recently, it has been reported that in endothelial cells, the rapid inhibition of the Ca 2ϩ -dependent NOS activity (e.g.…”

Section: Tyrosine Phosphorylation Inactivates Nos-i 9916mentioning

confidence: 99%

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Rapid Inactivation of NOS-I by Lipopolysaccharide Plus Interferon-γ-induced Tyrosine Phosphorylation

Colasanti

Persichini

Cavalieri

et al. 1999

Journal of Biological Chemistry

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Human astrocytoma T67 cells constitutively express a neuronal NO synthase (NOS-I) and, following administration of lipopolysaccharide (LPS) plus interferon-␥ (IFN␥), an inducible NOS isoform (NOS-II). Previous results indicated that a treatment of T67 cells with the combination of LPS plus IFN␥, by affecting NOS-I activity, also inhibited NO production in a very short time. Here, we report that under basal conditions, a NOS-I protein of about 150 kDa was weakly and partially tyrosine-phosphorylated, as verified by immunoprecipitation and Western blotting. Furthermore, LPS plus IFN␥ increased the tyrosine phosphorylation of NOS-I, with a concomitant inhibition of its enzyme activity. The same effect was observed in the presence of vanadate, an inhibitor of phosphotyrosine-specific phosphatases. On the contrary, genistein, an inhibitor of protein-tyrosine kinases, reduced tyrosine phosphorylation of NOS-I, enhancing its enzyme activity. Finally, using reverse transcriptase-polymerase chain reaction, we have observed that a suboptimal induction of NOS-II mRNA expression in T67 cells was enhanced by vanadate (or L-NAME) and inhibited by genistein. Because exogenous NO has been found to suppress NOS-II expression, the decrease of NO production that we have obtained from the inactivation of NOS-I by LPS/IFN␥-induced tyrosine phosphorylation provides the best conditions for NOS-II expression in human astrocytoma T67 cells.

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“…Previous studies have indicated that induction of iNOS expression may be kept suppressed by the endogenous NO level produced by a cNOS enzyme. In cell types possessing both cNOS and iNOS, this may represent a clear paradox (34). In view of these observations, it would be expected that the endogenous NO levels produced by cNOS could suppress iNOS expression in human PBMC and be incriminated for the lower nitrite concentrations released by human cells.…”

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Antigenic stimulation is more efficient than LPS in inducing nitric oxide production by human mononuclear cells on the in vitro granuloma reaction in schistosomiasis

Oliveira

Silva-Teixeira

Araújo-Filho

et al. 1999

Braz J Med Biol Res

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Nitric oxide (NO) is an extremely important and versatile messenger in biological systems. It has been identified as a cytotoxic factor in the immune system, presenting anti-or pro-inflammatory properties under different circumstances. In murine monocytes and macrophages, stimuli by cytokines or lipopolysaccharide (LPS) are necessary for inducing the immunologic isoform of the enzyme responsible for the high-output production of NO, nitric oxide synthase (iNOS). With respect to human cells, however, LPS seems not to stimulate NO production in the same way. Addressing this issue, we demonstrate here that peripheral blood mononuclear cells (PBMC) obtained from schistosomiasis-infected patients and cultivated with parasite antigens in the in vitro granuloma (IVG) reaction produced more nitrite in the absence of LPS. Thus, LPS-induced nitrite levels are easily detectable, although lower than those detected only with antigenic stimulation. Concomitant addition of LPS and L-N-arginine methyl ester (L-NAME) restored the ability to produce detectable levels of nitrite, which had been lost with L-NAME treatment. In addition, LPS caused a mild decrease of the IVG reaction and its association with L-NAME was responsible for reversal of the L-NAME-exacerbating effect on the IVG reaction. These results show that LPS alone is not as good an NO inducer in human cells as it is in rodent cells or cell lines. Moreover, they provide evidence for interactions between LPS and NO inhibitors that require further investigation.

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Bacterial Lipopolysaccharide Plus Interferon-Ɣ Elicit a Very Fast Inhibition of a Ca2+-dependent Nitric-oxide Synthase Activity in Human Astrocytoma Cells

Cited by 41 publications

References 27 publications

Nitric oxide mediates anti‐inflammatory action of extracorporeal shock waves

Nitric oxide mediates anti‐inflammatory action of extracorporeal shock waves

Rapid Inactivation of NOS-I by Lipopolysaccharide Plus Interferon-γ-induced Tyrosine Phosphorylation

Antigenic stimulation is more efficient than LPS in inducing nitric oxide production by human mononuclear cells on the in vitro granuloma reaction in schistosomiasis

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