Bacterial Levans of Intermediate Molecular Weight1

Mattoon, James R.; Holmlund, Chester E.; Schepartz, Saul A.; Vavra, James J.; Johnson, Marvin J.

doi:10.1128/aem.3.6.321-333.1955

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Survey Of Aldoses1

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1958

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Cited by 11 publications

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“…Oligofructoses [degree of polymerization (DP), 2-8] prepared from partial acid hydrolysates of both inulin and levan all failed to accept fructose at C-2 of the reducing-end unit. On the other hand, carbinol sites in some oligolevans are known to be active acceptors (Mattoon, Holmlund, Schepartz, Varva & Johnson, 1955;Avigad, 1956;Dedonder & Peaud-Lenoel, 1957;Peaud-Lenoel, 1957 b). This aspect will be the subject of a separate communication.…”

Section: Survey Of Aldosesmentioning

confidence: 99%

The mechanism of polysaccharide production from sucrose. 5. Transfer of fructose to C-1 of aldose by levansucrase

Hestrin

Avigad

1958

Biochemical Journal

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Section: Survey Of Aldosesmentioning

confidence: 99%

The mechanism of polysaccharide production from sucrose. 5. Transfer of fructose to C-1 of aldose by levansucrase

Hestrin

Avigad

1958

Biochemical Journal

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Continuous culture system for production of biopolymer levan using erwinia herbicola

Keith

Wiley

Ball

et al. 1991

Biotech & Bioengineering

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The optimal production of the fructan biopolymer levan by the bacterium Erwinia herbicola was investigated, including variations in nitrogen, carbon and phosphorous sources, pH, incubation time, culture yields up to 19% by weight produced based on conversion of sucrose as the carbon source when grown in a continuous culture system and processed by tangential flow filtration. Product identity was confirmed with gas chromatography (GC) and (13)C nuclear magnetic resonance (NMR). Gel permeation chromatography (GPC) and low-angle laser light scattering (LALLS) determination of the molecular weight of the product showed a significant difference in molecular weight values dependent on the method of analysis. Analysis by GPC resulted in molecular weight one order of magnitude lower than LALLS independent of sample, underscoring the unusual nature of this biopolymer.

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