Bacterial leakage in obturated root canals—part 2: a comparative histologic and microbiologic analyses

Brosco, Viviane Haiub; Bernardineli, Norberti; Torres, Sérgio Aparecido; Consolaro, Alberto; Bramante, Clóvis Monteiro; Moraes, Ivaldo Gomes de; Ordinola‐Zapata, Ronald; Garcia, Roberto Brandão

doi:10.1016/j.tripleo.2009.11.036

Cited by 28 publications

(38 citation statements)

References 27 publications

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“…[7] They also show high apical sealing capabilities, as showed in a microbiological model. [8] The constituents of these materials were identified by qualitative chemical analysis (X-ray Diffraction and EDX) and their relative percentages were determined by elemental microanalysis and chemical composition, using a slow dissolution process due to the low dissolving rate of GP. [23] Any resins and/or waxes present in the materials were not analyzed.…”

Section: Discussionmentioning

confidence: 99%

“…[1234] Several techniques have been proposed to obtain optimal adherence of gutta-percha (GP) to root canal walls[567] to minimize microbial leakage[8] and to ensure treatment success. [8910] Since the introduction of the warm vertical condensation technique by Schilder, in 1967,[10] a number of clinical placement techniques involving warm GP have been developed. [4911] However, there are no reports comparing different brands of thermoplasticized GP with respect to their chemical composition and thermal behavior.…”

Section: Introductionmentioning

confidence: 99%

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Chemical composition and thermal behavior of five brands of thermoplasticized gutta-percha

et al. 2013

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Objective:The aim of this study was determine the chemical composition and thermal behavior of Thermafil (TH), Microseal Cone (MC), Microseal Microflow (MF), Obtura (OB) and Obtura Flow (OF). In addition, their thermal behavior in response to temperature variations was studied by differential scanning calorimetry (DSC) to determine the temperature at which gutta-percha switches from the beta to alpha form, and from the alpha to the amorphous phase.Materials and Methods:The organic and inorganic fractions were separated by dissolution in chloroform. Gutta-percha (GP) was precipitated with acetone. The inorganic fraction was analyzed via Elemental Microanalysis. Energy Dispersive X-ray Microanalysis and X-ray Diffraction were used to identify the chemical elements and compounds (BaSO4 and ZnO). Thermal analysis was conducted using DSC.Results:The organic and inorganic fractions ranged from 21.3% and 26.9% of weights, respectively. MC and MF showed the highest percentages of organic compounds (P = 0.0125). All specimens exhibited two crystalline transformations when heated from ambient temperature to 130°C. MC presented the highest percentage of GP.Conclusions:No correlation was observed between chemical composition and thermal behavior. Each of the products showed thermal behavior that is typical of beta-phase gutta-percha.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemical composition and thermal behavior of five brands of thermoplasticized gutta-percha

et al. 2013

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“…Since the major cause of root canal treatment failures is E. faecalis bacteria, this strain was used in the present study (16). …”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of the sealing ability of three newly developed root canal sealers: A bacterial microleakage study

Jafari¹,

Sobhani²,

Kafil³

et al. 2016

J Clin Exp Dent

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BackgroundThe purpose of this study was to compare the sealing ability of MTA Fillapex, Apatite Root Canal Sealer and AH26 sealers.Material and MethodsThe present in vitro study was carried out on 142 extracted single-rooted human mature teeth. The teeth were randomly divided into three experimental groups (n=44) and two control groups (n=5). Three root canal sealers were MTA Fillapex, Apatite Root Canal Sealer and AH26. The teeth in the control groups were either filled with no sealer or made completely impermeable. The root canals were prepared and obturated with gutta-percha and one of the sealers. The teeth were sterilized with ethylene oxide gas prior to the bacterial leakage assessment using Enterococcus faecalis. Leakage was evaluated every 24 hours for 90 days. Data were analyzed with descriptive statistical methods and chi-squared test. If the data were significant, a proper post hoc test was used. Statistical significance was set at P<0.05.ResultsThe positive control specimens exhibited total bacterial penetration whilst the negative control specimens showed no evidence of bacterial penetration. At the end of the study, the analysis of microleakage with chi-squared test showed no significant differences between the experimental groups (P<0.05). The results of chi-squared test analyzing the pair-wise differences between the groups considering the numerical values for leakage day indicated the lowest leakage with AH26 and the highest with Apatite root sealer.ConclusionsAccording to the results of the present study, sealing ability of AH26 was significantly higher than that of MTA Fillapex and Apatite Root Canal Sealer. Key words:Mineral Trioxide aggregate, root canal obturation, dental seal.

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“…A reference E. faecalis strain was chosen because they are part of normal oral flora in humans and are frequently found in mixed infections with other aerobes and facultative anaerobes. [20212223] E. faecalis is also one of the most commonly isolated microbes from the root canal. [7] In this study, E. faecalis was selected due to ease of arrangement and interpretation of the data.…”

Section: Discussionmentioning

confidence: 99%

Lack of correlation between microbial penetration method and electro chemical technique for assessment of leakage through the root canal fillings

et al. 2014

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Aims:The purpose of this study was to compare microbial penetration method and electrochemical technique for evaluation of the apical seal.Materials and Methods:A total of 28 teeth were prepared using the step-back technique to ISO size 40 master apical files. The specimens were randomly divided into an experimental group, two positive and two negative controls. Root canals in the experimental and negative control group were filled with gutta-percha (GP) and sealer, using lateral condensation technique. In the positive control group, canals were filled with GP without any sealer. The external surface of each tooth was coated with two layers of the nail varnish, except for the access opening and the apical foramen. In the negative control group, the teeth were completely covered with nail varnish. The apical seal was recorded with two methods, each successively used on the same teeth: An electrochemical method and a bacterial penetration method.Statistical Analysis Used:The correlation of the electro chemical readings with the results obtained from bacterial micro leakage test was evaluated by Pearson's correlation coefficient.Results:Correlation coefficient of the measurements obtained from the two evaluation methods, was 0.23 (r = 0.23), so the correlation was not statistically significant (P = 0.275).Conclusions:This study shows that several studies by different methods are necessary before evaluation of the marginal leakage.

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Bacterial leakage in obturated root canals—part 2: a comparative histologic and microbiologic analyses

Cited by 28 publications

References 27 publications

Chemical composition and thermal behavior of five brands of thermoplasticized gutta-percha

Chemical composition and thermal behavior of five brands of thermoplasticized gutta-percha

In vitro evaluation of the sealing ability of three newly developed root canal sealers: A bacterial microleakage study

Lack of correlation between microbial penetration method and electro chemical technique for assessment of leakage through the root canal fillings

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