Bacterial leaf spot of hemp caused by Xanthomonas campestris pv. cannabis in Japan

Netsu, Osamu; Kijima, Toshio; Takikawa, Yuichi

doi:10.1007/s10327-013-0497-8

Cited by 5 publications

(11 citation statements)

References 7 publications

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“…cannabis ), isolated from symptomatic hemp leaves ( C. sativa L.), were chosen for genome sequencing. Type strain NCPPB 2877 was isolated by I. Sandru at the Lovrin station in the Timiş judeţ (Romania) in 1974 (Severin, 1978), and strain NCPPB 3753 was isolated by Y. Takiwawa in the Kanuma region of Tochigi Prefecture (Japan) in 1982 (isolate SUPP546; Netsu et al, 2014). X. cannabis strains were previously reported to cause disease on many dicot host plants.…”

Section: Results/methods/discussionmentioning

confidence: 99%

“…Symptoms associated with Xanthomonas bacterial leaf spot include water-soaking lesions followed by necrosis accompanied by a yellow halo (Severin, 1978; Netsu et al, 2014). The host range of these Xanthomonas strains appears to be quite large unlike most xanthomonads (Severin, 1978; Netsu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The host range of these Xanthomonas strains appears to be quite large unlike most xanthomonads (Severin, 1978; Netsu et al, 2014). Under laboratory conditions, these bacteria caused symptoms on a wide range of plants including cannabis, tomato, mulberry, geranium and Ficus erecta (Severin, 1978; Netsu et al, 2014). These strains further trigger an HR on tobacco, but do not elicit any response after inoculation on common bean (Severin, 1978; Netsu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Under laboratory conditions, these bacteria caused symptoms on a wide range of plants including cannabis, tomato, mulberry, geranium and Ficus erecta (Severin, 1978; Netsu et al, 2014). These strains further trigger an HR on tobacco, but do not elicit any response after inoculation on common bean (Severin, 1978; Netsu et al, 2014). The factors that contribute to pathogenicity and host range of cannabis-infecting Xanthomonas are unknown.…”

Section: Introductionmentioning

confidence: 99%

“…To gain insight into the evolution and pathogenicity of bacterial pathogens of cannabis, we sequenced two geographically distinct Xanthomonas strains, NCPPB 3753 and 2877, which were previously isolated from symptomatic hemp leaf tissue from Japan and Romania, respectively (Severin, 1978; Netsu et al, 2014). We tested their ability to infect barley, a previously unreported, compatible monocot host.…”

Section: Introductionmentioning

confidence: 99%

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Comparative genomics of a cannabis pathogen reveals insight into the evolution of pathogenicity in Xanthomonas

et al. 2015

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Pathogenic bacteria in the genus Xanthomonas cause diseases on over 350 plant species, including cannabis (Cannabis sativa L.). Because of regulatory limitations, the biology of the Xanthomonas-cannabis pathosystem remains largely unexplored. To gain insight into the evolution of Xanthomonas strains pathogenic to cannabis, we sequenced the genomes of two geographically distinct Xanthomonas strains, NCPPB 3753 and NCPPB 2877, which were previously isolated from symptomatic plant tissue in Japan and Romania. Comparative multilocus sequence analysis of housekeeping genes revealed that they belong to Group 2, which comprises most of the described species of Xanthomonas. Interestingly, both strains lack the Hrp Type III secretion system and do not contain any of the known Type III effectors. Yet their genomes notably encode two key Hrp pathogenicity regulators HrpG and HrpX, and hrpG and hrpX are in the same genetic organization as in the other Group 2 xanthomonads. Promoter prediction of HrpX-regulated genes suggests the induction of an aminopeptidase, a lipase and two polygalacturonases upon plant colonization, similar to other plant-pathogenic xanthomonads. Genome analysis of the distantly related Xanthomonas maliensis strain 97M, which was isolated from a rice leaf in Mali, similarly demonstrated the presence of HrpG, HrpX, and a HrpX-regulated polygalacturonase, and the absence of the Hrp Type III secretion system and known Type III effectors. Given the observation that some Xanthomonas strains across distinct taxa do not contain hrpG and hrpX, we speculate a stepwise evolution of pathogenicity, which involves (i) acquisition of key regulatory genes and cell wall-degrading enzymes, followed by (ii) acquisition of the Hrp Type III secretion system, which is ultimately accompanied by (iii) successive acquisition of Type III effectors.

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Section: Results/methods/discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative genomics of a cannabis pathogen reveals insight into the evolution of pathogenicity in Xanthomonas

et al. 2015

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Bacterial assemblages of urban microbiomes mobilized by runoff waters match land use typologies and harbor core species involved in pollutant degradation and opportunistic human infections

Bouchali

Mandon

Marti

et al. 2022

Science of The Total Environment

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Evolution of combinatorial diversity in trans-acyltransferase polyketide synthase assembly lines across bacteria

et al. 2021

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Trans-acyltransferase polyketide synthases (trans-AT PKSs) are bacterial multimodular enzymes that biosynthesize diverse pharmaceutically and ecologically important polyketides. A notable feature of this natural product class is the existence of chemical hybrids that combine core moieties from different polyketide structures. To understand the prevalence, biosynthetic basis, and evolutionary patterns of this phenomenon, we developed transPACT, a phylogenomic algorithm to automate global classification of trans-AT PKS modules across bacteria and applied it to 1782 trans-AT PKS gene clusters. These analyses reveal widespread exchange patterns suggesting recombination of extended PKS module series as an important mechanism for metabolic diversification in this natural product class. For three plant-associated bacteria, i.e., the root colonizer Gynuella sunshinyii and the pathogens Xanthomonas cannabis and Pseudomonas syringae, we demonstrate the utility of this computational approach for uncovering cryptic relationships between polyketides, accelerating polyketide mining from fragmented genome sequences, and discovering polyketide variants with conserved moieties of interest. As natural combinatorial hybrids are rare among the more commonly studied cis-AT PKSs, this study paves the way towards evolutionarily informed, rational PKS engineering to produce chimeric trans-AT PKS-derived polyketides.

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Bacterial leaf spot of hemp caused by Xanthomonas campestris pv. cannabis in Japan

Cited by 5 publications

References 7 publications

Comparative genomics of a cannabis pathogen reveals insight into the evolution of pathogenicity in Xanthomonas

Comparative genomics of a cannabis pathogen reveals insight into the evolution of pathogenicity in Xanthomonas

Bacterial assemblages of urban microbiomes mobilized by runoff waters match land use typologies and harbor core species involved in pollutant degradation and opportunistic human infections

Evolution of combinatorial diversity in trans-acyltransferase polyketide synthase assembly lines across bacteria

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