Bacterial inhibition ofOrobanche aegyptiacaandOrobanche cernuaradical elongation

Abstract

The ability of different bacteria to inhibit Orobanche radical elongation was examined. Seeds of Orobanche aegyptiaca Pers. and O. cernua Loefl. were induced to germinate with the Growth Regulator GR24 in the presence or absence of test bacterium. Radical lengths were estimated microscopically on a scale relative to seed length (0Á5 seed lengths) after 5 days of incubation at 258C. The results indicated that Pseudomonas aeruginosa QUBC1, P. fluorescens QUBC3, Bacillus atrophaeus QUBC16, and B. subtilis QUBC18 significantly inhibited radical elongation (P50.01) of both O. aegyptiaca and O. cernua relative to control radicals, whereas Microbacterium hydrocarbonoxydans QUBC11 and Ochrobactrum anthropi QUBC13 showed less inhibitory effects. Other bacterial isolates had no inhibitory effects. Bacterial isolates were identified using the universal method in addition to morphological and biochemical features. The establishment of the inhibitory effect of the most promising isolates, B. atrophaeus QUBC16 and P. aeruginosa QUBC1 on radical elongation of both Orobanche spp. is a step towards utilizing such bacteria as biocontrol agents against O. aegyptiaca, O. cernua, and potentially other Orobanche species.

IAA Producing Enterobacter sp. I-3 as a Potent Bio-herbicide Candidate for Weed Control: A Special Reference with Lettuce Growth Inhibition

“…Recently, Barghouthi and Salman [13] showed sever, lesser and no inhibitory effect of array of bacteria on broomrape plant growth. The number of DRBs belongs to the genus of Aeromonas, Agrobacterium, Alcaligenes, Bacillus, Burkholderia, Chryseomonas, Enterobacter, Microbacterium, Pseudomonas and Xanthomonas were reported by several studies [12][13][14]. On the other hand, the deleterious effect of bacterium on plants can be species or cultivars specific [15].…”

Induction of systemic resistance in tomato against broomrape (Phelipanche aegyptiaca)

“…Other strains of Bacillus have been able to inhibit broomrape considerably. Treating broomrape seedlings by Bacillus atrophaeus QUBC16 and B. subtilis QUBC18 reduced the radical elongation of broomrape significantly (Barghouthi & Salman, ). However, other rhizobacteria strains such as fluorescent pseudomonads also are able to suppress different broomrape species (Zermane, Souissi, Kroschel, & Sikora, ).…”

Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola

“…tritici (Wang et al 2015) besides this bacterium shows plant growth promoting activities on commercial wheat varieties (Batool et al 2016). Also, soil remediation and weed control properties were investigated by Barghouthi and Salman (2010). Surprisingly M. hydrocarbonoxydans bacterium is also carried diamino acid in the cell-wall peptidoglycan of BAS69T is lysine and of BNP48T is ornithine.…”