Bacterial Infection and Hypersensitive Response Assays in Arabidopsis-Pseudomonas syringae Pathosystem

Clubroot, caused by Plasmodiophora brassicae, is a major disease of crucifers. Effector proteins are important virulence factors in host recognition of pathogens and the interactions between pathogens and hosts. Secretory proteins, as effector candidates, have been studied in the interaction between Plasmodiophora brassicae and its hosts. In this study, 518 secretary proteins were screened from the Plasmodiophora brassicae genome. A total of 63 candidate effectors that induce or suppress cell death were identified using agroinfiltration-mediated transient expression in Nicothiana benthamiana. The candidate effectors, Pb4_102097 and Pb4_108104 showed high expressing level in the stage of rest spore maturity, could induce cell death and were associated with H2O2 accumulation in N. benthamiana leaves. In addition, 55 candidate effectors that could suppress BAX (Bcl-2-associated X protein) induced cell death, and 21 out of which could suppress the immunity caused by bacterial pathogen Pseudomonas syringae pv. tomato strain DC3000 expressing avrRps4 in Arabidopsis. Based on the expression pattern in different stages, 28 candidate effectors showed high expression levels during the primary and secondary infection stage. Five candidate effectors containing the RXLR motif functioned in the cytoplasm and cell membrane.

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“…Six leaves were selected for evaluate for bacterial disease assay. And the methods followed the procedures described by Yuan and Xin (2021) .…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of putative effectors from Plasmodiophora brassicae that suppress or induce cell death in Nicotiana benthamiana

Zhan

Liu²,

Yang³

et al. 2022

Front. Plant Sci.

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“…The pervasiveness of C Las and its profound impact on citrus crops underscore the necessity of an in-depth exploration of the PPIs between the host plants and the pathogen. Recently Yuan et al [7] mapped a network of Arabidopsis thaliana interactions with the bacterial pathogen Pseudomonas syringae , identifying new components involved in the plant immune response and paving the way for future plant disease control strategies. In a similar vein, Dyer et al [8] conducted a large-scale study on PPIs between human cells and Bacillus anthracis , the bacterium responsible for anthrax, revealing numerous potential therapeutic intervention targets.…”

Section: Introductionmentioning

confidence: 99%

Deep-HPI-pred: An R-Shiny applet for network-based classification and prediction of Host-Pathogen protein-protein interactions

Tahir ul Qamar,

Noor,

Guo

et al. 2024

Computational and Structural Biotechnology Journal

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“…Moreover, the ROS burst can also strengthen the plant cell walls through oxidative cross-link of polymers to inhibit pathogen growth [ 11 , 12 , 13 ]. While HR is a rapid cell death phenomenon induced by pathogen or their metabolites, occurring at the point of pathogen penetration to prevent further pathogen spreading [ 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. The plant immune system is also regulated by plant hormones, especially jasmonic acid (JA) and SA, which play an important role in basic resistance against various plant pathogens.…”

Section: Introductionmentioning

confidence: 99%

Ectopic Expression of HbRPW8-a from Hevea brasiliensis Improves Arabidopsis thaliana Resistance to Powdery Mildew Fungi (Erysiphe cichoracearum UCSC1)

Liu

et al. 2022

IJMS

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The RPW8s (Resistance to Powdery Mildew 8) are atypical broad-spectrum resistance genes that provide resistance to the powdery mildew fungi. Powdery mildew of rubber tree is one of the serious fungal diseases that affect tree growth and latex production. However, the RPW8 homologs in rubber tree and their role of resistance to powdery mildew remain unclear. In this study, four RPW8 genes, HbRPW8-a, b, c, d, were identified in rubber tree, and phylogenetic analysis showed that HbRPW8-a was clustered with AtRPW8.1 and AtRPW8.2 of Arabidopsis. The HbRPW8-a protein was localized on the plasma membrane and its expression in rubber tree was significantly induced upon powdery mildew infection. Transient expression of HbRPW8-a in tobacco leaves induced plant immune responses, including the accumulation of reactive oxygen species and the deposition of callose in plant cells, which was similar to that induced by AtRPW8.2. Consistently, overexpression of HbRPW8-a in Arabidopsis thaliana enhanced plant resistance to Erysiphe cichoracearum UCSC1 and Pseudomonas syringae pv. tomato DC30000 (PstDC3000). Moreover, such HbRPW8-a mediated resistance to powdery mildew was in a salicylic acid (SA) dependent manner. Taken together, we demonstrated a new RPW8 member in rubber tree, HbRPW8-a, which could potentially contribute the resistance to powdery mildew.

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Bacterial Infection and Hypersensitive Response Assays in Arabidopsis-Pseudomonas syringae Pathosystem

Cited by 5 publications

References 12 publications

Identification and characterization of putative effectors from Plasmodiophora brassicae that suppress or induce cell death in Nicotiana benthamiana

Identification and characterization of putative effectors from Plasmodiophora brassicae that suppress or induce cell death in Nicotiana benthamiana

Deep-HPI-pred: An R-Shiny applet for network-based classification and prediction of Host-Pathogen protein-protein interactions

Ectopic Expression of HbRPW8-a from Hevea brasiliensis Improves Arabidopsis thaliana Resistance to Powdery Mildew Fungi (Erysiphe cichoracearum UCSC1)

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