Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Abstract

Biofield therapies have been reported to improve the quality of life as compared to other energy medicine. The aim of the study was to evaluate the impact of Mr. Trivedi's biofield energy treatment on Pseudomonas fluorescens (P. fluorescens) for antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. P. fluorescens cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 49838) number and divided in control and treated group. The effect was evaluated on day 10, and 159 after biofield treatment in lyophilized state. Further study was performed on day 5, 10, and 15 after retreatment on day 159 in revived state as per study design. All experimental parameters were studied using automated MicroScan Walk-Away ® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. fluorescens with other bacterial species after treatment. The results showed improved sensitivities and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in revived and lyophilized treated sample with respect to the control. Arginine, cetrimide, kanamycin, and glucose showed altered biochemical reactions after biofield treatment with respect to control. Biotype numbers were altered along with species in lyophilized as well as in revived group. Based on nucleotides homology and phylogenetic analysis using 16S rDNA gene sequencing, treated sample was detected to be Pseudomonas entomophila (GenBank Accession Number: AY907566) with 96% identity of gene sequencing data, which was nearest homolog species to P. fluorescens (Accession No. EF672049). These findings suggest that Mr. Trivedi's unique biofield treatment has the capability to alter changes in pathogenic P. fluorescens even in the lyophilized storage condition and can be used to modify the sensitivity of microbes against antimicrobials.

Consciousness Energy Healing Treatment on <i>Withania somnifera</i> Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

“…The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a Complementary and Alternative Medicine (CAM) health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, meditation, Qi Gong, Tai Chi, chiropractic/osteopathic manipulation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, hypnotherapy, relaxation techniques, pilates, healing touch, movement therapy, rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, cranial sacral therapy, Reiki, and applied prayer (as is common in all religions, like Hinduism, Buddhism, Christianity, and Judaism) [25]. The Biofield Energy Treatment (The Trivedi Effect ® ) has been extensively studied with significant outcomes in many scientific fields such as cancer research [26], biotechnology [27,28], genetics [29,30], altered antimicrobial sensitivity of pathogenic microbes in microbiology [31][32][33], altered structure of the atom in relation to the various metals, polymers, ceramics, and chemicals materials science [34][35][36], altered physical and chemical properties of organic compounds [37][38][39], pharmaceuticals [40,41], nutraceuticals [42,43], and improved overall growth and yield of plants in agricultural science [44,45]. Herbal extracts and it's formulations despite of their outstanding in vitro results exhibited poor or low in vivo activity, because of their low lipid solubility or improper molecular size, causing in deprived absorption and thus poor bioavailability.…”

Assessment of the Consciousness Energy Healing Treated <i>Withania Somnifera</i> (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy

“…The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a Complementary and Alternative Medicine (CAM) health care approach in addition to other therapies, medicines and practices such as natural products, yoga, deep breathing, Tai Chi, chiropractic/osteopathic manipulation, Qi Gong, meditation, special diets, homeopathy, progressive relaxation, massage, guided imagery, acupressure, relaxation techniques, hypnotherapy, acupuncture, healing touch, movement therapy, pilates, mindfulness, rolfing structural integration, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, cranial sacral therapy, Reiki, and applied prayer (as is common in all religions, like Hinduism, Christianity, Buddhism and Judaism) [19]. The Trivedi Effect ® -Biofield Energy Treatment has been extensively studied with significant outcomes in many scientific fields such as cancer research [20]; altered antimicrobial sensitivity of pathogenic microbes in biotechnology [21,22], genetics [23,24], microbiology [25][26][27], changing the structure of the atom in relation to the various metals, ceramics, polymers and chemicals materials science [28][29][30], nutraceuticals [31,32], pharmaceuticals [33,34], organic compounds [35][36][37], and improved overall growth and yield of plants in agricultural science [38,39].…”

A Comprehensive Physical, Spectroscopic, and Thermal Characterization of <i>Withania somnifera</i> (Ashwagandha) Root Extract Treated with the Energy of Consciousness (The Trivedi Effect<sup>®</sup>)

“…The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a Complementary and Alternative Medicine (CAM) health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, pilates, rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, cranial sacral therapy and applied prayer (as is common in all religions, like Christianity, Hinduism, Buddhism and Judaism) [25]. The Biofield Energy Treatment (The Trivedi Effect ® ) has been extensively studied with significant outcomes in many scientific fields such as cancer research [26], altered antimicrobial sensitivity of pathogenic microbes in microbiology [27][28][29], biotechnology [30,31], genetics [32,33], changing the structure of the atom in relation to the various metals, ceramics, polymers and chemicals materials science [34][35][36], altered physical and chemical properties of pharmaceuticals [37,38], nutraceuticals [39,40], organic compounds [41][42][43], and improved overall growth and yield of plants in agricultural science [44,45]. Herbal extracts and herbal drugs despite of their outstanding in vitro results exhibited poor or negligible in vivo activity, because of their reduced lipid solubility or improper molecular size, causing deprived absorption and thus poor bioavailability [1].…”