Bacterial Identification by Infrared Spectrophotometry

Riddle, Jackson W.; Kabler, Paul W.; Kenner, Bernard A.; Bordner, Robert H.; Rockwood, Sue W.; Stevenson, Heber J. R.

doi:10.1128/jb.72.5.593-603.1956

Cited by 52 publications

(11 citation statements)

References 21 publications

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“…The use of infrared absorption spectra of bacteria as an aid to their identification seemed promising to Stevenson and Bolduan in 1952. Since then, Levine et al (1953b) and Riddle et al (1956) in the United States and Norris (1953) and Thomas and Greenstreet (1954) in England have come to similar conclusions. Inasmuch as these investigators worked primarily with animal pathogenic and enteric microorganisms, we undertook to broaden the scope of inquiry.…”

mentioning

confidence: 75%

Certain Factors Affecting the Infrared Spectra of Selected Microorganisms1

Haynes¹,

Melvin²,

Locke³

et al. 1958

Applied Microbiology

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mentioning

confidence: 75%

Certain Factors Affecting the Infrared Spectra of Selected Microorganisms1

Haynes¹,

Melvin²,

Locke³

et al. 1958

Applied Microbiology

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“…The use of infrared spectroscopy to differentiate bacteria has been studied since the 1950's [26,29]. Unfortunately, due to the weak performances of dispersive spectrometers, these kinds of studies ceased in the 1970's.…”

Section: Introductionmentioning

confidence: 99%

Potentiality of Fourier Transform Infrared Spectroscopy (FTIR) for discrimination and identification of dairy Lactic acid bacteria

Amiel

Mariey

Curk-Daubié³

et al. 2000

Lait

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The potentiality of FTIR spectroscopy for the identification of lactic acid bacteria (LAB) used in the dairy industry was tested. For this purpose, spectra of different strains were recorded using standardized conditions. These strains were species of Lactobacillus (12 species, 3 subspecies), Lactococcus (4 species, 3 subspecies), Leuconostoc (3 species, 3 subspecies), Weissella (1 species) and Streptococcus (2 species) involved in the soft cheese industry. Spectral libraries were then established by statistical analysis. Reference strains were then tested on these libraries, and 100% of correct identifications were obtained at the genus and species level, 86% at the subspecies level. Well-identified strains were included in libraries. Wild isolates (n = 48) previously identified by biochemical tests and RAPD method were then tested. We obtained 100% at the genus level and 69% at the species level of correlation between FTIR and other methods. Finally, spectra of reference strains performed in other laboratories were tested with the same method, and the results tallied with official identification in 100% of the cases at the genus level and in 41% of the cases at the species level. These results concerning a few strains allow us to plan the development of a more complete database for rapid identification of LAB. FTIR spectroscopy / lactic acid bacteria / classification / identificationRésumé -Potentialités de la spectroscopie infrarouge à transformée de Fourier (IRTF) pour la discrimination et l'identification de bactéries lactiques d'intérêt laitier. La discrimination et l'identification de souches de bactéries lactiques d'origine laitière ont été étudiées par spectroscopie IRTF. Les spectres de 28 souches de bactéries lactiques appartenant aux genres Lactobacillus (12 espèces, 3 sous-espèces), Lactococcus (4 espèces, 3 sous-espèces), Leuconostoc (3 espèces, 3 sousespèces), Weissella (1 espèce) et Streptococcus (2 espèces) ont été enregistrés dans des conditions Lait 80 (2000) 445-459 445

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“…Physiological and morphological studies and infrared spectrophotometric analysis (Riddle et al, 1956) of whole cell suspensions have shown the mixed cultures to contain species belonging to the genera Pseudomonas, Flavobacterium, and Achromobacter. The presence of pseudomonad Relationship of sample source and Ky.…”

Section: Resultsmentioning

confidence: 99%