Bacterial hydrogen production in recombinant Escherichia coli harboring a HupSL hydrogenase isolated from Rhodobacter sphaeroides under anaerobic dark culture

Transferring [NiFe] hydrogenase gene from Rhodopeseudomonas palustris into E. coli BL21(DE3) for improving hydrogen production

“…Soo Youn Lee [2] found that hydrogenase from Rhodobacter sphaeroides, a [NiFe] hydrogenase, can improve hydrogen production in E. coli and showed that recombinant E. coli can evolve about 6.3 L hydrogen/one mol glucose. Mark A.…”

“…Hydrogen is a renewable, efficient, and clean [1] energy which can be an alternative to fossil fuels [2]. Compared with chemically generated hydrogen, the production condition of biological hydrogen occurs at normal pressure and temperature [3] and has minimal impact on the environment.…”

“…Hydrogenase catalyzes the reaction 2H þ þ 2e À 4 H 2 [5] and nitrogenase catalyzes the reaction N 2 þ 8H þ þ 8e À þ 16ATP 4 2NH 3 þ H 2 þ 16ADP þ 16Pi during nitrogen fixation [6]. Moreover, nitrogenase has a higher activity than hydrogenase in R. palustris [2,4]. In fact, membrane-bound [NiFe] hydrogenase consists of both a small and large subunit encoded by hupS and hupL, respectively.…”

Effect of mixed organic compounds extracted from Rhodobacter sphaeroides on Daphnia magna (water flea)

“…Rhodobacter sphaeroides KCTC1434 (Korean Collection for Type Culture) was allowed to grow in 250 ml Sistrom's minimal medium (Lee et al 2010), incubated aerobically in the light or dark for 48 h. Light (at 15 W/m 2 ) or 48 h dark at 30°C under 180 rev/min shaking. The cells were centrifuged at 3,500g for 15 min.…”

“…In addition, a few of these purple non-sulfur bacteria produce substantial quantities of useful materials including amino acids, nucleic acid material, a vitamin and bio-active substances (Barry et al 1995;Lee et al 2010Lee et al , 2011.…”