Bacterial growth monitored by two-dimensional tandem mass spectrometry

Szalwinski, Lucas J.; Gonzalez, L. Edwin; Morato, Nicolás M.; Marsch, Brett; Cooks, R. Graham

doi:10.1039/d1an01901e

Cited by 13 publications

(7 citation statements)

References 36 publications

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“…This approach allows the capture of all precursor ion/product ion information in the 2D MS/MS data domain in times on the order of 1.2 s per scan using a single population of trapped ions. 2D MS/MS has previously been applied to monitoring bacterial lipid profiles and their changes over time, as well as for the analysis of complex mixtures of fentanyls and sulfonamides. , Two-dimensional mass spectrometry was initially reported in 1987 by Gäumann and co-workers and others using a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) . Two-dimensional mass spectrometry (2D-MS) has been applied since in the analysis of polymers, top-down/bottom-up analysis of calmodulin, and bottom-up analysis of IgG 1 antibodies .…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

et al. 2022

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Lipidomic and metabolomic profiles of sporulated and vegetative Bacillus subtilis and Bacillus thuringiensis from irradiated lysates were recorded using a quadrupole ion trap mass spectrometer modified to perform two-dimensional tandem mass spectrometry (2D MS/MS). The 2D MS/MS data domains, acquired using a 1.2 s scan of negative ions generated by nanoelectrospray ionization of microwave irradiated spores, showed the presence of dipicolinic acid (DPA) as well as various lipids. Aside from microwave radiation to extract DPA and lipids from spores, sample preparation was minimal. Characteristic lipid and metabolic profiles were observed using 107108 cells of the two Bacillus species. Major features of the lipid profiles observed for the vegetative states included sets of phosphatidylglycerol (PG) lipids. Product ion spectra were extracted from the 2D MS/MS data, and they provided structural information on the fatty acid components of the PG lipids. The study demonstrates the flexibility, speed, and informative power of metabolomic and lipidomic fingerprinting for identifying the presence of spore-forming biological agents using 2D MS/MS as a rapid profiling screening method.

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Section: Introductionmentioning

confidence: 99%

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

et al. 2022

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“… 153 In this PS‐IMS‐MS study, the technique was able to differentiate five different Bactillus species after only 4 h of cell incubation and a 2 min analysis time. Characterization of phospholipids in bacterial cultures has also been achieved with DESI‐MS, achieving an impressive sample analysis time of less than 10 s. 154 The analysis of bacterial species has primarily targeted smaller metabolites produced by microbes, though a recent study used LESA‐MS for the detection of proteins in E. coli colonies. 155 Through the combination of electroporation to release proteins from cells followed by LESA‐MS, intact protein assemblies up to 50 kDa could be detected directly from growing colonies.…”

Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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“…51 Further integration of this approach with novel MS/MS scan methodologies such as 2D MS/MS (all fragments from all precursors) provides additional information that can be utilized in the generation of spectral databases, a recent example being the analysis of microorganisms. 75 ■ CONCLUDING REMARKS Twenty years after its inception, DESI has found numerous applications across a wide variety of fields, a trend that should continue as the technology evolves. Of particular interest is its integration into standard of practice workflows for intraoperative diagnosis, particularly for real-time IDH genotyping of brain tumors, as well as its implementation in automated drug discovery.…”

Section: ■ Chemical Reactions In Desi Microdropletsmentioning

confidence: 99%

“…Moreover, high-throughput analysis of biological samples has also been achieved using high-density tissue arrays, an application that can efficiently support intraoperative MS diagnostics through the generation of classification models or biomarker discovery based on large sample cohorts . Further integration of this approach with novel MS/MS scan methodologies such as 2D MS/MS (all fragments from all precursors) provides additional information that can be utilized in the generation of spectral databases, a recent example being the analysis of microorganisms …”

Section: High-throughput Desimentioning

confidence: 99%

Desorption Electrospray Ionization Mass Spectrometry: 20 Years

Morato,

Cooks

2023

Acc. Chem. Res.

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Conspectus Mass spectrometry (MS) is one of the most widely used technologies in the chemical sciences. With applications spanning the monitoring of reaction products, the identification of disease biomarkers, and the measurement of thermodynamic parameters and aspects of structural biology, MS is well established as a universal analytical tool applicable to small compounds as well as large molecular complexes. Regardless of the application, the generation of gas-phase ions from neutral compounds is a key step in any MS experiment. However, this ionization step was for many years limited to high-energy approaches that required gas-phase analytes and thus it was restricted to volatile samples. Over the last few decades, new methodologies have been developed to address this limitation and facilitate ionization of biological molecules. Electrospray ionization (ESI) is the most broadly used of these methods, as it facilitates the ionization of intact polar compounds from solution. Twenty years ago, our group reported a new ionization method that uses a charged solvent spray to impact a surface, generating ions from objects rather than just solutions and doing so directly in the ambient environment with no vacuum requirements and little to no sample preparation. This method was termed desorption electrospray ionization (DESI), and it initiated a new field that would come to be known as ambient mass spectrometry. The simplicity and wide applicability of the DESI technologyand the tens of ambient ionization methods developed subsequentlyrevolutionized the MS analysis of complex materials for their organic components, especially for in situ applications. This Account describes the history of DESI, starting with the development of the technique from early electrosonic spray ionization (ESSI) experimental observations as well as the studies leading to the understanding of its mechanism as a “droplet pick-up” phenomenon involving sequential events (i.e., thin film formation, solid–liquid extraction, secondary droplet generation, and ESI-like ionization from these droplets). We also overview the developments and applications of the technology that have been demonstrated by our group during the last two decades. In particular, we describe (i) the use of DESI for tissue imaging, one of its more significant applications to date, and its extension to intraoperative clinical diagnosis; (ii) the integration of the technology with portable instrumentation for in situ analysis, especially when coupled with tandem mass spectrometry (MS/MS); (iii) the use of DESI microdroplets as microvessels to accelerate organic reactions by orders of magnitude compared to those in bulk solution; and (iv) the combination of all these capabilities for automated high-throughput experiments aimed at accelerating drug discovery.

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Bacterial growth monitored by two-dimensional tandem mass spectrometry

Abstract: The growth of the bacterium E. coli was monitored by targeting the phospholipid constituents through desorption electrospray ionization and characterizing individual sets of isomers by recording the full 2D MS/MS...

Cited by 13 publications

References 36 publications

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

Applications of ambient ionization mass spectrometry in 2022: An annual review

Desorption Electrospray Ionization Mass Spectrometry: 20 Years

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