Bacterial, Fungal, and Viral Disease Resistance Loci Mapped in a Recombinant Inbred Common Bean Population (`Dorado'/XAN 176)

Abstract

<jats:p>Understanding the genomic associations among disease resistance loci will facilitate breeding of multiple disease resistant cultivars. We constructed a genetic linkage map in common bean (<jats:italic>Phaseolus vulgaris</jats:italic> L.) containing six genes and nine quantitative trait loci (QTL) comprising resistance to one bacterial, three fungal, and two viral pathogens of bean. The mapping population consisted of 79 F<jats:sub>5:7</jats:sub> recombinant inbred lines (RILs) derived from a `Dorado'/XAN 176 hybridization. There were 147 randomly amplified polymorphic DNA (RAPD) markers, two sequence characterized amplified region (SCAR) markers, one intersimple sequence repeat (ISSR) marker, two seedcoat color genes <jats:italic>R</jats:italic> and <jats:italic>V</jats:italic>, the <jats:italic>Asp</jats:italic> gene conditioning seed brilliance, and two rust [<jats:italic>Uromyces appendiculatus</jats:italic> var. <jats:italic>appendiculatus</jats:italic> (Pers.:Pers) Unger] resistance genes: one conditioning resistance to Races 53 and 54 and the other conditioning resistance to Race 108. These markers mapped across eleven linkage groups, one linked triad, and seven linked pairs for an overall map length of 930 cM (Kosambi). Genes conditioning resistance to anthracnose (<jats:italic>Co-2</jats:italic>) [<jats:italic>Colletotrichum lindemuthianum</jats:italic> (Sacc. and Magnus) Lams.-Scrib.], bean rust (<jats:italic>Ur-5</jats:italic>), and bean common mosaic virus (<jats:italic>I</jats:italic> and <jats:italic>bc-3</jats:italic>) (BCMV) did not segregate in this population, but were mapped by inference using linked RAPD and SCAR markers identified in other populations. Nine previously reported quantitative trait loci (QTL) conditioning resistance to a variety of pathogens including common bacterial blight [<jats:italic>Xanthomonas campestris</jats:italic> pv. <jats:italic>phaseoli</jats:italic> (Smith) Dye], ashy stem blight [<jats:italic>Macrophomina phaseolina</jats:italic> (Tassi) Goid.], and bean golden mosaic virus (BGMV), were located across four linkage groups. Linkage among QTL for resistance to ashy stem blight, BGMV, and common bacterial blight on linkage group B7 and ashy stem blight, BGMV, and rust resistance loci on B4 will complicate breeding for combined resistance to all four pathogens in this population.</jats:p>

Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding

“…Symbols in subscript represent the source population of the QTL. AG: A55/G 122 , BA: Belneb-RR-1/A55 Fourie et al, 2004;Jung et al, 2003), BE: Berna/EMP 419 (Murray et al, 2004a,b), BG: BAT 881/G 21212 (Frei et al, 2005), BJ: BAT 93/Jalo EEP558 (Freyre et al, 1998;Gepts, 1999;Geffroy et al, 2000), BH: BAC 6/HT 7719 (Jung et al, 1996), BN: Bunsi/Newport (Kolkman & Kelly, 2003), BR: Bunsi/Raven (Ender & Kelly, 2005), B60: Benton/NY6020-4 (Miklas et al, 2003b), DG: DOR 364/G 19833 (López et al, 2003), DX: DOR 364/XAN 176 (Miklas et al, 2000c), H95: HR67/OAC 95 (Yu et al, 2004), MF: Montcalm/FR266 (Schneider et al, 2001), PX: PC50/XAN 159 (Jung et al, 1997(Jung et al, , 1998Park et al, 2001), RN: Red Hawk/Negro San Luis (Román-Avilés & , S95: Seaforth/OAC 95 (Tar'an et al, 2001), and XC: XR-235-1-1/Calima (Yu et al, 1998). Gene and QTL locations are approximate because most were not directly mapped in the BAT 93/Jalo EEP558 population.…”

“…The I gene located on B2 , is independent of recessive resistance conditioned by three different bc genes. The bc-3 gene is located on B6 (Johnson et al, 1997;Miklas et al, 2000c;Mukeshimana et al, 2005), whereas the bc-1 2 allele was mapped to B3 . The non-specific bc-u allele, needed for expression of bc-2 2 resistance, also resides on B3 based on the loose linkage with the bc-1 locus (Strausbaugh et al, 1999).…”

Common Bean Rust: Pathology and Control

“…2005;Liebenberg et al 2006b (continued) Miklas et al 2000Miklas et al , 2002 Gene symbols as proposed by Kelly et al 1996. The reader is also referred to the BIC Web site list of genes: www.css.msu.edu/bic/PDF/Bean% 20Genes%20List%202008.pdf.…”

“…There are several genetic maps on which many of the rust resistance genes have been mapped; for instance, Gepts et al (1993);Nodari et al (1993);McClean et al (1994) (Ur-6); Jung et al (1996) (Ur-BAC-6); Freyre et al 1998 (Bean Core Map); Jung et al (1998) (Ur-9 and Ur-12); Miklas et al (1998) (Ur-5); Miklas et al (2000) (Ur-Dorado-53, Ur-Dorado-108, Ur-5 marker); Miklas et al (2002) (Ur-3, Ur-4, Ur-5, Ur-9, Ur-11, Ur-12, Ur-Dorado-53, Ur-Dorado-108 The Bean Core ('BAT 93'/'Jalo EEP 558') map has been expanded to accommodate most of these, and other, genes . Chromosome numbers, allocated in 2003 by Pedrosa et al (2003), have been changed to correspond with those used for the Core Map linkage groups by Freyre et al (1998), Pedrosa-Harand et al (2006), and Pedrosa et al (2008).…”

A dominant gene for garnet brown seed coats at the Rk locus in ‘Dorado’ common bean and mapping Rk to linkage group 1

“…The dominant red seed coat color of 'Dorado' (Fig. 1) was presumed to be controlled by R (Miklas et al 2000), but this gene has an interaction with B that differs from the known gene interaction of R with G B v, which expresses dark seal brown (nearly black) seed coat color. Two hypotheses could fit the preliminary data.…”