Bacterial Fruit Blotch of Watermelon: Association of the Pathogen with Seed

Rane, K. K.; Latin, RX

doi:10.1094/pd-76-0509

Cited by 94 publications

(52 citation statements)

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“…citrulli and the fact that BFB losses are often associated with infested seeds or transplants, continued crop losses have resulted in litigation against several seed companies by watermelon growers in the US (Schaad et al 2003). Therefore, many attempts have been made to develop effective means of coping with BFB, including seed treatments (Rane and Latin 1992;Hopkins et al 2003), detection of the pathogen in contaminated seeds (Walcott et al 2006;Bahar et al 2008), and chemical treatments in the field (Hopkins 1991(Hopkins , 1995. Despite their contribution to combating the disease, the efficiency of these approaches is limited, and BFB still poses a serious threat to the watermelon and melon industries.…”

Section: Introductionmentioning

confidence: 99%

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

2008

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Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax avenae subsp. citrulli, is a serious threat to the watermelon and melon industries. To date, there are no commercial cultivars of cucurbit crops resistant to the disease. Here we assessed the level of tolerance to bacterial fruit blotch of various commercial cultivars as well as breeding and wild lines of melon, using seed-transmission assays and seedling-inoculation experiments. Selected cultivars were also tested in a greenhouse experiment with mature plants. All tested cultivars/lines were found to be susceptible to the pathogen, and most of them showed different responses (relative tolerance vs. susceptibility) in the different assays; however, some consistent trends were found: cv. ADIR339 was relatively tolerant in all tested assays, and cv. 6407 and wild lines BLB-B and EAD-B were relatively tolerant in seed-transmission assays. We also provide evidence supporting a strong correlation between the level of susceptibility of a cultivar/line and the ability of the pathogen to adhere to or penetrate the seed. To the best of our knowledge, this is the first attempt to assess melon cultivars/lines for bacterial fruit blotch response.

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Section: Introductionmentioning

confidence: 99%

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

2008

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“…By using pathogen-resistant or abiotic stress-resistant cultivars watermelon yields can be increased. The cultivars have been developed by traditional breeding to have resistance to watermelon fruit blotch (Rane and Latin 1992), to watermelon mosaic virus (Gillaspie and Wright 1993), and to zucchini yellows mosaic virus (Boyhan et al 1992). Recent advances in insertion of bacterial, fungal and virus resistance genes through Agrobacterium-mediated transformation would facilitate the development of new disease resistant genotypes without significantly altering the genetic composition, and have made it possible to improve their productivity and quality beyond the limit of traditional breeding.…”

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confidence: 99%

Agrobacterium-mediated transformation in Citrullus lanatus

Cho¹,

Moon²,

Liu³

et al. 2008

Biol. plant.

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Agrobacterium tumefaciens-mediated transformation was used to produce transgenic watermelon. Cotyledonary explants of Citrullus lanatus Thumb (cv. Daesan) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing pPTN289 carrying with bar gene and pPTN290 carrying with nptII gene, respectively. There was a significant difference in the transformation frequency between bacteria strains and selective markers. The EHA101/pPTN289 showed higher transformation frequency (1.16 %) than GV3101/pPTN289 (0.33 %) and LBA4404/pPTN289 or /pPTN290 (0 %). The shoots obtained (633 and 57 lines) showed some resistance to glufosinate and paromomycin, respectively. Of them, the β-glucuronidase positive response and PCR products amplified by bar and nptII specific primers showed at least 21 plants resistant to glufosinate and at least 6 plants to paromomycin. Southern blot analysis revealed that the bar gene integrated into genome of transgenic watermelon. Acclimated transgenic watermelons were successfully transplanted in the greenhouse and showed no phenotypic variation.

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“…Rane and Latin (1992) obtained 80 % (naturally infested seed) and 96 % (laboratory infested seed) reductions in seed transmission with hot water treatment (50 C, 20 min). Kubota et al (2012) using dry heat (85 C, 3-5 days) claimed complete disinfection.…”

Section: Bacterial Blotch Of Cucurbitsmentioning

confidence: 93%

Non-chemical Seed Treatment in the Control of Seed-Borne Pathogens

Koch¹,

Roberts²

2014

Global Perspectives on the Health of Seeds and Plant Propagation Material

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Non-chemical seed treatments include physical treatments, microbial treatments and treatments with other agents of natural origin like plant powders or extracts. Physical treatments with hot water, aerated steam, or dry heat have successfully been applied to a range of crops against a range of target pathogens and are in commercial use primarily for vegetable seeds. They can be very effective but need to be optimised on a per seed lot basis.Microbial seed treatments may control not only seed-borne pathogens but also provide some protection against pathogenic soil-borne inoculum. However, research on the use of micro-organisms as seed treatments has been limited, and there are only a few examples of commercial use. The latter is also true for botanical seed treatments, despite many reports of bactericidal and fungicidal effects of compounds from plants. The reason may be a lack of research on the one hand but mainly commercial constraints like development and registration costs in relation to market size.The current chapter gives an overview of approaches that have been taken to utilize the above-described non-chemical methods for control of important seedborne pathogens of vegetables and small grain cereals. The examples treated include bacterial (black rot of brassicas, pea bacterial blight, bacterial blotch of cucurbits, black chaff of cereals), fungal (Alternaria diseases of carrot, black leg of brassicas, common bunt of wheat, Fusarium seedling diseases of small grain cereals, the loose smuts of barley and wheat, fungal diseases of rice and sorghum) and important viral diseases.

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Bacterial Fruit Blotch of Watermelon: Association of the Pathogen with Seed

Cited by 94 publications

References 0 publications

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

Agrobacterium-mediated transformation in Citrullus lanatus

Non-chemical Seed Treatment in the Control of Seed-Borne Pathogens

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