Bacterial flora on hands of nursing staff as a source of health care associated infections at a tertiary care centre

I, Vedavati B.; H, Halesh L.

doi:10.18231/2394-5478.2019.0007

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…In this study, Gram positive bacteria were found to occur more than Gram negative bacteria. Most skin flora bacteria are Gram positive, which would account for their predominance on hand swab samples which was in accordance with the findings of Vedavati and Halesh 19 who also demonstrated that the Gram positive bacteria predominate over Gram negative bacteria from the growth positive hand samples.…”

Section: Original Research Articlesupporting

confidence: 90%

“…In most of the positive samples, they showed polymicrobial growth. The isolation rate of the pathogens was lower than previous study carried out by Kumari et al 18 but comparable with the findings of Vedavati and Halesh 19 . Among the total isolates of 219 obtained from overall growth positive samples in this study 92/219 (42.01%) isolates were Gram negative bacteria and 127/219 (57.99%) were Gram positive bacteria.…”

Section: Original Research Articlesupporting

confidence: 88%

“…This study also highlighted the presence of potential pathogenic Gram-negative bacteria in hands of HCWs like Klebsiella spp 32(34.78%) followed by Escherichia coli 17(18.48%), Pseudomonas aeruginosa 12(13.04%), Acenetobacter spp 11(11.96%), Proteus spp 9(9.78%), Citrobacter spp 7(7.61%) and Enterobacter spp 4(4.35%). Similar types of pathogens were found on hands of health care workers in studies done by Vedabati and Halesh 19 and Kumari et al 18 . The presence of these pathogenic bacteria on hands poses a potential risk to vulnerable, immunecompromised individuals.…”

Section: Original Research Articlesupporting

confidence: 81%

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Prevalence of Extended Spectrum Beta Lactamase Producing and Carbapenems Resistance Isolates from Hands of Health Care Workers in a Health Care Setting of Nepal

Sharma

2020

J. Gandaki Med. Coll. Nepal

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Background: Hands of healthcare workers (HCWs) could be colonized by potential drug resistant bacteria like Extended Spectrum Beta Lactamase producers (ESBLs) and Carbapenems-resistant (CR) isolates and could become vectors of nosocomial pathogens in healthcare facilities that are associated with an increase of morbidity, mortality and healthcare costs. This study aimed to investigate the prevalence of ESBLs and CR isolates from hands of HCWs with their antibiotic susceptibility pattern. Materials and methods: This was a cross-sectional study that included a total of 150 hand swabs collected from March, 2018 to September, 2018 in Gandaki Medical College and Teaching Hospital. Isolation, identification and antimicrobial susceptibility tests were done using standard microbiological procedures. Results: Among the total isolates of 219 obtained from growth positive samples 92/219(42.01%) were Gram negative bacteria (GNB) and the most common were Klebsiella spp 32(34.78%) followed by Escherichia coli 17(18.48%), Pseudomonas aeruginosa12 (13.04%), Acenetobacter spp 11(11.96%), Proteus spp 9(9.78%), Citrobacter spp 7(7.61%) and Enterobacter spp 4(4.35%). The prevalence of ESBLs, CR and ESBLs with Co-resistant to Carbapenems were 19.56%,14.13% and 9.78%, respectively. The most effective drugs for isolates were Nitrofurantoin followed by Amikacin, Tetracycline and Gentamycin. Distribution pattern of the ESBLs and CR isolates among doctors, nurses, laboratory technicians, helpers and basic science faculties were not significant (p>0.05). Conclusions: This report revealed the emerging and moderately high prevalence of ESBLs, CR and ESBLs with Co-resistant to Carbapenems GNB with their antibiotic susceptibility patterns found on hands of HCWs in Nepal. Thus, this study could be helpful in developing proper guidelines on hand hygiene and implementation of infection control measures including contact precautions against the spread of infections by such pathogens in healthcare settings.

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Section: Original Research Articlesupporting

confidence: 90%

Section: Original Research Articlesupporting

confidence: 88%

Section: Original Research Articlesupporting

confidence: 81%

See 1 more Smart Citation

Prevalence of Extended Spectrum Beta Lactamase Producing and Carbapenems Resistance Isolates from Hands of Health Care Workers in a Health Care Setting of Nepal

Sharma

2020

J. Gandaki Med. Coll. Nepal

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“…The contaminated hands of healthcare workers play an important role in the transmission of hospital infections and are a potential source of infections related to healthcare services (Vedavati & Halesh, 2019). This study results showed that on entry to the hospital, there was production at the rate of 93.7% in the least washed region of the right hand, and this rate fell to 74.6% on leaving the hospital.…”

Section: Discussionmentioning

confidence: 69%

Which Micro-Organisms are Transferred by Healthcare Personnel Between Hospital and Home?

2023

JHMN

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The most important factor in the spread of hospital infections is the hands of healthcare personnel. The aim of ths study was to determine which micro-organisms are transferred from home to hospital, and which from hospital to home, by the hands of healthcare personnel. Material and Methods: The sample universe of this research was the healthcare personnel in a tertiary level public hospital. A total of 10 doctors and 53 nurses, selected at random according to the clinics where they worked, were included. The study data were collected in two stages. In the first stage, the study participants completed a personal information form. In the second stage, samples were taken from the right and left hands of the healthcare personnel on entering and leaving the hospital. The samples were isolated and seeded in culture media. Bacteria identification and antibiotic sensitivity were determined using a BD Phoenix 100 automated system. Results: According to the samples taken, there was determined to be greater bacteria production on the hands of the healthcare personnel when entering the hospital. The greatest production was in the least washed area of the right hand (93.7% on entry, 74.6% on exit). Nurses and those working in surgical clinics were seen to have greater bacteria production on both entry to and exit from the hospital compared to other healthcare workers. The bacteria most produced were MSSE, Micrococcus, Staphylococcus strains, Streptococcus strains and MRSE. In the examination of antibiotic sensitivity, the antibiotics to which the produced bacteria were most sensitive were amikacin, ciprofloxacin, daptomycin, gentamicin levoflaxocin, teicoplanin, linezolid teicoplanin, trimetoprim/sulfamethoxazole and vancomycin. Conclusion:The study results showed that just as healthcare personnel transferred some micro-organisms from home to hospital on their hands, they also transferred some bacteria to home on leaving the hospital. This is of great importance in respect of the spread of hospital infections. With the necessary precautions taken to prevent the transfer of micro-organisms to or from hospital, the prevalence of hospital infections will decrease.

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Effectiveness of Hand Hygiene in Reducing Bacterial Flora in Health Care Workers-a Study of Impact

Singh

Mane

Sangwan³

2020

ijsr

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Introduction: Hand hygiene is simplest, most effective and least expensive tool to prevent health care worker associated infection. The compliance among HCW is low due to various reasons leading to increased morbidity and mortality in Healthcare associated infection (HAI). Proper hand hygiene significantly reduces bacterial flora and HAI. The study was planned with the aim to know the pattern of microbial flora and its drug resistance and effectiveness of hand hygiene in reducing bacterial load on hands of health care workers. Material and Methods: Both hand finger imprints of 50 participants were taken on Blood agar before and after hand hygiene. The plates were incubated 24-48 hours at 37°C.Colony count was done. Bacteria were identified by their colony characteristics and biochemical reactions. AST was performed as per CLSI 2019 guidelines. Results: 90-99% reduction in bacterial colony count after hand hygiene was seen in majority of participants Mixed growth was seen in ten samples. Micrococcus was most common organism isolated in twenty-four samples, followed by diptheroids in sixteen samples, Coagulase negative staphylococcus (CoNS) in fourteen samples and Staphylococcus aureus in six samples .Three CoNS and four Staphylococcus aureus were Methicillin resistant. Gram negative bacilli isolated were Klebsiella species, Pseudomonas species and Escherichia Coli each in two samples respectively One Escherichia Coli was ESBL producer. All the GNB were sensitive to carbapenem. After performing hand hygiene micrococcus was isolated from four sample, diptheroids from three and CONS from one sample. Staphylococcus aureus and GNB were not found after hand hygiene Conclusion: HCW hands are colonized with bacteria. Proper hand hygiene technique awareness and its compliance significantly reduce bacterial load and decrease HAI.

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Bacterial flora on hands of nursing staff as a source of health care associated infections at a tertiary care centre

Cited by 3 publications

References 6 publications

Prevalence of Extended Spectrum Beta Lactamase Producing and Carbapenems Resistance Isolates from Hands of Health Care Workers in a Health Care Setting of Nepal

Prevalence of Extended Spectrum Beta Lactamase Producing and Carbapenems Resistance Isolates from Hands of Health Care Workers in a Health Care Setting of Nepal

Which Micro-Organisms are Transferred by Healthcare Personnel Between Hospital and Home?

Effectiveness of Hand Hygiene in Reducing Bacterial Flora in Health Care Workers-a Study of Impact

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