Bacterial fingerprinting of faecal samples of pigs supplemented with plant sourced feed additives

Abstract

The present study was aimed to see the faecal bacterial fingerprints of pigs applying terminal restriction fragment length polymorphism (T-RFLP) analysis. Sixteen crossbred (Large White Yorkshire X desi) grower pigs (body weight of 15.8±1.1 kg), were divided into four groups (control, antibiotic, herbal residue and prebiotic) with four in each treatment. T-RFLP analysis revealed comparable bacterial abundance in control - antibiotic and herbal residue - prebiotic groups. Abundance of Peptostreptococcus anaerobius (an antibiotic resistant pathogen) was higher in antibiotic supplemented pigs. Number of significantly responded operational taxonomic units (OTU) was higher in herbal residue and prebiotic supplemented pigs than control or antibiotic pigs. Bacterial abundance was significantly higher in pigs supplemented with prebiotic, followed by herbal residue, antibiotic, and control. The abundance of beneficial bacteria such as Sarcina maxima, Bacteroidetes sp., Cetobacterium somerae, Selenomonas sputigera, Fecalibacterium prausnitzi were significantly higher in prebiotic groups. The present findings established that herbal residue and prebiotic are alternative to feed antibiotic in pigs.

The pig gut microbiota analysis techniques, a comparison

“…As advantages this technique offer cheap, rapid and semi-quantitative results. It does not allow however for phylogenetic identification this being the main disadvantage of T-RFLP but it can be remediated by pairing 16S rRNA clone library analysis with T-RFLP (Samanta et al, 2019).…”

Supplemental Cap-Met in diet to promote growth performance and immune response of nursery pigs

“…The use of antibiotics such as Colistin has been recognized to control gut pathogens and improve growth performance. Since antibiotic resistance genes regarding bacteria have spread worldwide, the European Union has totally banned the use of antibiotics in farm animals as growth and health promoters (Samanta et al, 2018), including Colistin (Mohamed et al, 2016).…”