Bacterial Exopolysaccharide mediated heavy metal removal: A Review on biosynthesis, mechanism and remediation strategies

Gupta, Pratima; Diwan, Batul

doi:10.1016/j.btre.2016.12.006

Cited by 636 publications

(280 citation statements)

References 87 publications

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“…We observed an increase in carbohydrate content (0.2 to 89 % considering 1g/l as 100% yield) in cell-free broth up to 30 ppm metal concentration for lead and chromium while, in presence of 40 ppm cadmium, maximum EPS yield was detected (table 2). Metal-induced EPS synthesis has previously reported in various microorganisms [22]. along with an increase in EPS levels (as mentioned above) suggest a significant role of EPS in metal tolerance.…”

Section: Resultsmentioning

confidence: 92%

“…The interaction might immobilize metal ions. Thus, as suggested by Gupta and Diwan EPS production can be considered as constitutive nonspecific mechanism displayed by microorganisms in the response of heavy metals [22]. [27].…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, heavy metal tolerance of BRI 36 in terms of maximum tolerable concentration (MTC) was found to increase considerably with an increase in EPS produced by the isolate. Role of EPS in metal resistance has been previously documented by various researchers [14][15][16][17]. These observations prompted us to undertake studies on EPS production, and its effect on heavy metal tolerance of Kocuria sp.…”

Section: Introductionmentioning

confidence: 99%

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Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Mulik

Bhadekar

2018

Int J Pharm Pharm Sci

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Objective: Evaluation of Extracellular Polymeric Substance (EPS) induced heavy metal tolerance in Kocuria sp. BRI 36.Methods: Initially, the effect of different concentrations of glucose (1-10 %) on EPS production by BRI 36 was examined. At optimum glucose concentrations, EPS levels were measured by varying heavy metal concentrations (10-50 ppm) of Pb 2+ , Cd 2+ and Cr 3+Results: Kocuriasp. BRI 36 produced maximum EPS (1g/l) at 5% glucose. Increase in EPS production up to 89% (considering 1g/l as 100%) with an increase in concentrations of heavy metals up to 40 ppm. MTC levels of BRI 36 for heavy metals increased up to 700 ppm when it was cultivated in presence of 5% glucose indicating a major role of surface polymer in metal adsorption. The function of EPS as a protective cover was also evident from an increase in survival percentage of BRI 36 up to 39.4 at MTC. Comparative analysis of extracted crude EPS by FTIR revealed the involvement of O-H, C=O, and C-O-C groups in metal adsorption . Maximum tolerable concentration (MTC) and survival percentage of BRI 36 were determined under conditions that support EPS synthesis. Comparative analysis of extracted crude EPS was performed by Fourier Transform Infrared Spectroscopy (FTIR) to establish functional groups involved in the metal interaction. Conclusion:Antarctic oceanic isolate Kocuria sp. BRI 36 has an ability to produced EPS under stress conditions of heavy metals. Simultaneously, its MTC values increased due to increase in EPS levels. These observations suggest the possibility to develop gentle, environmentally safe and costeffective method for heavy metal removal.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Mulik

Bhadekar

2018

Int J Pharm Pharm Sci

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“…In our experiments; we observed that the YG4 EPS at 0.5 mg/mL concentration could remove 80.94 ± 1.56% uranyl ions from its aqueous solution. The interaction between EPS molecules and metal ions has been ascribed to electrostatic interactions and coordination bond formation (Gupta and Diwan, 2017;Mohite et al, 2017). This property of removing uranyl ions can play an important role in the reduction of uranium-induced nephrotoxicity by either decreasing the absorption and distribution of uranyl ions or increasing the rate of their excretion.…”

Section: Uranium Compounds Dissociate Into Uranyl Ions [(Uo 2 ) 2+mentioning

confidence: 99%

Uranium-induced Cytotoxicity in HEK 293T Cells is reduced by a ß-glucan type Exopolysaccharide produced by Enterobacter sp. YG4

2018

japs

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Uranium-induced nephrotoxicity is a major health concern and countering it is essential. In this regard; a β-glucan type exopolysaccharide produced by Enterobacter sp. YG4 was tested for its capacity to reduce the uranyl nitrateinduced cytotoxicity in Human embryonic kidney cell line (HEK 293T). This exopolysaccharide at 0.5 mg/mL concentration was able to remove 80.94% of uranyl ions from its aqueous solution. Experiments with HEK 293T cells showed that the exposure to 0.05 mg/mL uranyl nitrate resulted in 22.13% cytotoxicity and treatment with YG4 exopolysaccharide could reduce the cytotoxicity of uranyl nitrate significantly. These results highlight the potential of this exopolysaccharide in counteracting the nephrotoxic effects of uranium.

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“…It is well known for producing valuable biological compound like carbohydrates, lipids, proteins and pigments [6], and found in diverse habitats including freshwater, marine environment, hot springs, hypersaline localities, cold and arid deserts [7]. Both the types of EPS are composed of carbohydrate and non-carbohydrate constituents like protein, lipid, sulfate, uronic acid and pyruvate [8]. Presence of sulfate and uronic acid in EPS contribute negative charge and provide sticky behavior to the organisms [9], [10].Basically, EPS is involved in protecting the cell from drastic environmental changes [11].…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Marine Filamentous Heterocyst Cyanobacterium producing Higher EPS Coupled with Higher Viscosity

Arulraj¹

2018

IJRASET

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Marine cyanobacterial exopolysaccharides are natural biopolymers well known for their potentials in various fields. As there is an increase in demand for a new polymer, fifteen (15) strains of different morphotypes unicellular, filamentous and filamentous heterocystous belonging to varied habitat were screened for EPS production. EPS synthesis was found to be coupled with incubation duration, growth phase, which reflected on the rheology of the medium. Filamentous heterocystous strain Nostoc sp BDU 80591 of salt pan habitat yielded (2.8 gL -1 ) EPS exhibited (4.64 cP) as viscosity.

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Bacterial Exopolysaccharide mediated heavy metal removal: A Review on biosynthesis, mechanism and remediation strategies

Cited by 636 publications

References 87 publications

Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Uranium-induced Cytotoxicity in HEK 293T Cells is reduced by a ß-glucan type Exopolysaccharide produced by Enterobacter sp. YG4

Identification of Potential Marine Filamentous Heterocyst Cyanobacterium producing Higher EPS Coupled with Higher Viscosity

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