Bacterial enzymes for lignin depolymerisation: new biocatalysts for generation of renewable chemicals from biomass

Bugg, Timothy D. H.; Williamson, James J.; Rashid, Goran M. M.

doi:10.1016/j.cbpa.2019.11.007

Cited by 88 publications

(57 citation statements)

References 51 publications

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“…The subsequent catabolic products (e.g. pyruvate, acetyl-coA, oxaloacetate, fumarate and/or succinate) can enter the tricarboxylic acid cycle (TCA) to generate energy [8,9,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Lignin Catabolism Potential of Soil-Derived Lignocellulolytic Microbial Consortia by a Gene-Centric Metagenomic Approach

2020

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An exploration of the ligninolytic potential of lignocellulolytic microbial consortia can improve our understanding of the eco-enzymology of lignin conversion in nature. In this study, we aimed to detect enriched lignin-transforming enzymes on metagenomes from three soil-derived microbial consortia that were cultivated on "pre-digested" plant biomass (wheat straw -WS1-M, switchgrass -SG-M and corn stover -CS-M). Of 60 selected enzyme-encoding genes putatively involved in lignin catabolism, 20 genes were significantly abundant in WS1-M, CS-M and/or SG-M consortia compared with the initial forest soil inoculum metagenome (FS1). These genes could be involved in lignin oxidation (e.g. superoxide dismutases), oxidative stress responses (e.g. catalase/peroxidases), generation of protocatechuate (e.g. vanAB genes), catabolism of gentisate, catechol and 3phenylpropionic acid (e.g. gentisate 1,2-dioxygenases, muconate cycloisomerases and hcaAB genes), the beta-ketoadipate pathway (e.g. pcaIJ genes) and tolerance to lignocellulose-derived inhibitors (e.g. thymidylate synthases). The taxonomic affiliation of 22 selected lignin-transforming enzymes from WS1-M and CS-M consortia metagenomes revealed that Pseudomonadaceae, Alcaligenaceae, Sphingomonadaceae, Caulobacteraceae, Comamonadaceae and Xanthomonadaceae are the key bacterial families in the catabolism of lignin. We sketched out a predictive "model" where each microbial population has the potential to metabolize an array of aromatic compounds through different pathways, suggesting that lignin catabolism can follow a "task division" strategy.Here, we have established an association between functions and taxonomy, allowing a better 2 understanding of lignin transformations in soil-derived lignocellulolytic microbial consortia, and pinpointing some bacterial taxa and catabolic genes as ligninolytic trait-markers.

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“…The subsequent catabolic products (e.g. pyruvate, acetyl-coA, oxaloacetate, fumarate and/or succinate) can enter the tricarboxylic acid cycle (TCA) to generate energy [8,9,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Lignin Catabolism Potential of Soil-Derived Lignocellulolytic Microbial Consortia by a Gene-Centric Metagenomic Approach

2020

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“…In the recognition of the biological molecule the flurochrome is used which is used to produce the light in the sample. Some of the molecules which does not have the spectral properties in the fluorescence based transducer the dye is used in the sample which attached with the recognize element and when the chemical reaction take place in the sample they emit light which can be measured with the help of the transducer [28,29].…”

Section: Fluorescence Basedmentioning

confidence: 99%

Review on Biochemical Detection of Biological, Plant Samples Using Integrative Approach

Hussain¹,

Ghafoor²,

Rauf³

et al. 2021

Scholars Bulletin

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Biosensor is the system which is generally consists of the system which recognizes the biological samples in appropriate manner. Bio receptor makes interaction with the analytic and transducer help in the amplification of the signals and measureable signals can be displayed on the screen in the form of the electrical signals. Bio-receptor is a biological molecule such as the enzyme, antibody, protein, and nucleic acid which is commonly present in the living organism which is used in the recognition of the biological molecule. Different techniques play as the key role in the technologies for the specificity, for seeking the measurement analyte make interaction with the electrode of the sensor. Biological signal based Biosensor can be categorized as Immuno-biosensor, Enzyme Based biosensor, Nucleic acid /DNA based biosensor and Cellular based biosensor. In the body with the high specificity and the sensitivity the ability of to detect the physiological and the pathogenic related molecules offers a opportunity which is very powerful and helpful in the treatment of the disease with the early diagnosis. The metals such nickel, cobalt which play essential role and act as the catalyst and also stabilize the structure of biological molecule such as the bacterial cell wall and the proteins. The metals in the high concentration cause the toxicity in the blood.

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“…Finally, we note that this review is focused on the conversion of the carbohydrate fractions of feedstocks to commodity chemicals in a biorefinery. In practice, for commercial viability both the carbohydrate and the lignin fractions will be converted to both commodity chemicals and biofuels and this will involve both chemo-and biocatalytic methods in water (Rinaldi et al, 2016;Bugg et al, 2019).…”

Section: Acid-catalyzed Dehydration Of Carbohydrates To Furan Derivatmentioning

confidence: 99%

Catalytic Oxidations in a Bio-Based Economy

Sheldon

2020

Front. Chem.

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The role of bio-and chemo-catalytic aerobic oxidations in the production of commodity chemicals in a bio-refinery is reviewed. The situation is fundamentally different to that in a petrochemicals refinery where the feedstocks are gaseous or liquid hydrocarbons that are oxidized at elevated temperatures in the vapor or liquid phase under solvent-free conditions. In contrast, the feedstocks in a biorefinery are carbohydrates that are water soluble solids and their conversion will largely involve aerobic oxidations of hydroxyl functional groups in water as the solvent under relatively mild conditions of temperature and pressure. This will require the development and use of cost-effective and environmentally attractive processes using both chemo-and biocatalytic methods for alcohols and polyols.

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Bacterial enzymes for lignin depolymerisation: new biocatalysts for generation of renewable chemicals from biomass

Abstract: Please refer to published version for the most recent bibliographic citation information. If a published version is known of, the repository item page linked to above, will contain details on accessing it.

Cited by 88 publications

References 51 publications

Exploring the Lignin Catabolism Potential of Soil-Derived Lignocellulolytic Microbial Consortia by a Gene-Centric Metagenomic Approach

Exploring the Lignin Catabolism Potential of Soil-Derived Lignocellulolytic Microbial Consortia by a Gene-Centric Metagenomic Approach

Review on Biochemical Detection of Biological, Plant Samples Using Integrative Approach

Catalytic Oxidations in a Bio-Based Economy

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