Bacterial Energy Metabolism

Ward, B. Q.

doi:10.1016/b978-0-12-397169-2.00011-1

Cited by 24 publications

(15 citation statements)

References 76 publications

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“…Cytochrome bd oxidases, catalyze the two-electron oxidation of either ubiquinol or menaquinol are at the end of the respiratory chain where they couple with the Na + -translocating NADH:quinone oxidoreductase (Na + -NQR) and other dehydrogenases such as NADH-linked lactate dehydrogenase [71–74]. The Na + -NQR system also acts as a primary sodium pump and respiratory module at bacterial membrane that mediates electron transfer from NADH to quinone, which is coupled with the translocation of sodium ions across membrane generating a sodium motive force [75, 76] or chemiosmosis [77–84]. Here, during growth at the subsurface of soft agar, genes predicted to encode a Na + -NQR system were upregulated.…”

Section: Resultsmentioning

confidence: 99%

Amino acids as wetting agents: surface translocation by Porphyromonas gingivalis

et al. 2019

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Our understanding of how oral microbiota adapt in response to changes in their surroundings remains limited. This is particularly true of the slow-growing anaerobes that persist below the gum line. Here, we report that the oral anaerobe Porphyromonas gingivalis strain 381 can surface translocate when sandwiched between two surfaces. We show that during movement, this bacterium alters its metabolism, specifically side products of arginine utilization including citrulline and ornithine accumulated in the translocating cells; while arginine, N-acetyl-arginine, and the polyamine putrescine, which is produced from arginine were consumed. In addition, our results indicate that movement requires modification of the surrounding environment via proteolysis, cell dispersion, cell-on-cell rolling, and sub-diffusive cell-driven motility. We also show that production of fimbriae and fimbriae-associated proteins; as well as the regulation of contact-dependent growth inhibition genes, which are known to be involved in self-nonself discrimination, and the type IX secretion system are central to surface translocation. These studies provide a first glimpse into P. gingivalis motility and its relationship to ecological variables.

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Section: Resultsmentioning

confidence: 99%

Amino acids as wetting agents: surface translocation by Porphyromonas gingivalis

et al. 2019

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“…Energy metabolism is vital for physiological processes and biochemical pathways for driving division and cell growth in microbes such as bacteria [ 56 , 57 ]. Interactions in mixed microbial cultures are driven by metabolite exchanges and are dependent on symbiotic and sometimes competitive behaviours [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli

et al. 2021

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Microbial life in extreme environments, such as deserts and deep oceans, is thought to have evolved to overcome constraints of nutrient availability, temperature, and suboptimal hygiene environments. Isolation of probiotic bacteria from such niche may provide a competitive edge over traditional probiotics. Here, we tested the survival, safety, and antimicrobial effect of a recently isolated and potential novel strain of Bacillus subtilis (CP9) from desert camel in vitro. Antimicrobial assays were performed via radial diffusion, agar spot, and co-culture assays. Cytotoxic analysis was performed using pig intestinal epithelial cells (IPEC-J2). Real time-PCR was performed for studying the effect on ETEC virulence genes and metabolomic analysis was performed using LC-MS. The results showed that CP9 cells were viable in varied bile salts and in low pH environments. CP9 showed no apparent cytotoxicity in IPEC-J2 cells. CP9 displayed significant bactericidal effect against Enterotoxic E. coli (ETEC), Salmonella Typhimurium, and Methicillin-resistant Staphylococcus aureus (MRSA) in a contact inhibitory fashion. CP9 reduced the expression of ETEC virulent genes during a 5 h co-culture. Additionally, a unique emergent metabolic signature in co-culture samples was observed by LC-MS analysis. Our findings indicate that CP9 exhibits a strong antibacterial property and reveals potential mechanisms behind.

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“…Subsequently, the proton motive force is converted to adenosine triphosphate (ATP). The key enzyme, F0F1 ATPase, consists of a hydrophobic F0 component (formed from integral membrane proteins A, B and C) and a hydrophilic F1 component (formed from five subunits α, β, λ, δ and ϵ) (68). According to our results, the expression of the whole F0 component and at least two subunits (δ (SCO5370) and ϵ(SCO5374)) of the F1 component were also found to be controlled by HrdB.…”

Section: Discussionmentioning

confidence: 99%

DNA mapping and kinetic modeling of the HrdB regulon inStreptomyces coelicolor

Šmídová

Ziková

Pospíšil

et al. 2018

Nucleic Acids Research

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HrdB in streptomycetes is a principal sigma factor whose deletion is lethal. This is also the reason why its regulon has not been investigated so far. To overcome experimental obstacles, for investigating the HrdB regulon, we constructed a strain whose HrdB protein was tagged by an HA epitope. ChIP-seq experiment, done in 3 repeats, identified 2137 protein-coding genes organized in 337 operons, 75 small RNAs, 62 tRNAs, 6 rRNAs and 3 miscellaneous RNAs. Subsequent kinetic modeling of regulation of protein-coding genes with HrdB alone and with a complex of HrdB and a transcriptional cofactor RbpA, using gene expression time series, identified 1694 genes that were under their direct control. When using the HrdB–RbpA complex in the model, an increase of the model fidelity was found for 322 genes. Functional analysis revealed that HrdB controls the majority of gene groups essential for the primary metabolism and the vegetative growth. Particularly, almost all ribosomal protein-coding genes were found in the HrdB regulon. Analysis of promoter binding sites revealed binding motif at the −10 region and suggested the possible role of mono- or di-nucleotides upstream of the −10 element.

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Bacterial Energy Metabolism

Cited by 24 publications

References 76 publications

Amino acids as wetting agents: surface translocation by Porphyromonas gingivalis

Amino acids as wetting agents: surface translocation by Porphyromonas gingivalis

Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli

DNA mapping and kinetic modeling of the HrdB regulon inStreptomyces coelicolor

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