Bacterial Endophytic Communities in the Grapevine Depend on Pest Management

Campisano, A.; Antonielli, Livio; Pancher, Michael; Yousaf, Sohail; Pindo, Massimo; Pertot, I.

doi:10.1371/journal.pone.0112763

Cited by 121 publications

(96 citation statements)

References 74 publications

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“…In this first version of the model we did not consider some important contributors to BN, including insect vectors and wild reservoirs of 'Ca. P. solani' or even bacterial endophytic communities in grapevine (Campisano et al 2014). Therefore, in the future more complex models with more variables for each element may be constructed using similar approaches.…”

Section: Discussionmentioning

confidence: 99%

Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field

et al. 2017

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Bois noir (BN) is the most widespread European grapevine yellows disease caused by 'Candidatus Phytoplasma solani'. Although our knowledge of the mechanisms of interactions of this pathogenic bacteria with host is largely unknown, the plant-pathogen system of BN is commonly used as a model system for studying grapevine yellows diseases. We applied here a conceptual model of general plant pathology -a disease triangle for describing interactions among the host plant, the pathogen and the environment. We generated a proofof-concept statistical model for disease triangle using original experimental data and different statistical and data mining approaches for a selected system of 'Ca. P. solani' infection of cv. 'Chardonnay' grapevine plants. We monitored individual plants from a single vineyard over a period of six years. Phytoplasma content, the expression of 21 selected grapevine genes and environmental conditions were recorded and related to disease severity. Our model predicts that in described conditions BN is a function of the expression of grapevine gene VvDMR6, summer rainfall and abundance of 'Ca. P. solani'. The greatest impact among elements of the disease triangle is attributed to the pathogen, and is independent of the pathogen titer. We showed that this first de facto representation of the disease triangle is useful for showing disease dynamics over several years and could be applied to other plant-pathogen systems. The overall results of this study will contribute to understanding of 'Ca. P. solani' biology and its interactions with grapevine host.

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Section: Discussionmentioning

confidence: 99%

Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field

et al. 2017

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“…Campisano et al [23] used a 700 bp region that includes the domains from V5 up to V9 in order to assess the impact of pest management on bacterial endophytic communities of Merlot and Chardonnay grapevines, with the results indicating abundance differences of operational taxonomic units (OTUs) between organic and intergrated pest management (IPM) grapevines. The same genomic region was also targeted by Perazzolli et al [24] in a study of leaf microbiota, that resulted in identification of beneficial microbial communities that could be used as a tool for crop protection.…”

Section: Amplified Genomic Regionsmentioning

confidence: 99%

Massive Sequencing: A New Tool for the Control of Alcoholic Fermentation in Wine?

et al. 2018

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Abstract:In wine industry, there is a prevalent use of starter cultures to promote a controlled and efficient alcoholic fermentation preventing the growth of spoilage microbes. However, current trends in enology aim to combine the guaranteed success of monitored process and the complexity of fermentations either by inoculating autochthonous starters or by performing spontaneously to produce distinctive wines. To understand the complex roles of microorganisms on wine fermentation, we must understand their population dynamics and their relationships with wine quality and metabolome. Current metagenomics techniques based on massive sequencing are gaining relevance to study the diversity and evolution of microbial population on every stage of the wine making process. This new tool and technique increases the throughput and sensitivity to study microbial communities. This review focuses on the current knowledge about wine alcoholic fermentation, the contribution of massive sequencing techniques and the possibility of using this tool for microbial control.

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“…Campisano et al (2014) studied endophytic fungal composition in grapevines cultivated using organic production and Integrated Pest Management (IPM). They observed Mesorhizobium, Caulobacter and Staphylococcus genera as dominant endophytes in organic vineyards, while Ralstonia, Burkholderia and Stenotrophomonas were more abundant in grapevines from IPM vineyards.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Tea Endophytic Fungi: Cultivar- And Tissue Preferences

Win¹,

Matsumura²,

Fukuda³

2018

Appl. Ecol. Env. Res.

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Abstract. Endophytic fungi were isolated from four healthy tissues (new leaf, old leaf, bark, and xylem) of three Japanese tea cultivars (Hokumei, Sayamakaori, and Yabukita) at the Saitama Tea Research Institute, Japan in July 2015. A total of 520 isolates was obtained from 600 segments and were classified into 44 fungal taxa; the majority (93.2%) belonged to the phylum Ascomycota. The lowest infection rate was found in the xylem tissue of all cultivars. The total infection rate did not differ significantly among the cultivars. Colletotrichum gloeosporioides f. sp. camelliae and Pleosporales sp. were the predominant endophytes in all tissue types and cultivars. Most of the dominant endophytes showed obvious cultivar and tissue preferences. Tissue type played a more important role in shaping community structure than did cultivar. Colletotrichum gloeosporioides f. sp. camelliae preferred bark and old leaf tissue while Pleosporales sp. preferred new leaf tissue. The colonization frequency of C. gloeosporioides f. sp. camelliae was significantly lower in the Yabukita cultivar. Stem tissues harbored more diverse endophytes than did leaf tissues.

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Bacterial Endophytic Communities in the Grapevine Depend on Pest Management

Cited by 121 publications

References 74 publications

Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field

Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field

Massive Sequencing: A New Tool for the Control of Alcoholic Fermentation in Wine?

Diversity of Tea Endophytic Fungi: Cultivar- And Tissue Preferences

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