Bacterial Dynamics in the Accessory Nidamental Gland of &lt;i&gt;Sepioteuthis lessoniana&lt;/i&gt; throughout Maturation

Yang, Shan‐Hua; Chen, Chi; Hsieh, Yunli Eric; Yang, Sung Yin; Li, Hau Wen; Ching, Tzu Yun; Wang, Chia Hui; Chang, Ching Fong; Tang, Sen Lin; Wu, Guan Chung

doi:10.1264/jsme2.me21030

Cited by 5 publications

(6 citation statements)

References 40 publications

(68 reference statements)

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“…Furthermore, the microbiota of the ANG undergoes a shift during female maturation of the bigfin reef squid (Yang et al, 2021) and Hawaiian bobtail squid (Kerwin et al, 2021). Our previous study further suggested that the environment affects the microbial compositions of individuals, whereas maturity levels exert progressive effects on microbial dynamics over time (Yang et al, 2021). These host-selected bacteria are transmitted to the egg jelly coat in some squid and cuttlefish (Kerwin and Nyholm, 2017;Huang et al, 2018).…”

Section: Control Of Mutualistic Microbiota In Squid By Innate Immunitymentioning

confidence: 80%

“…The ANG houses a bacterial consortium that is horizontally transmitted from the environmental community in bigfin reef squid (Li et al, 2019;Li et al, 2022) and other coleoid cephalopods (Kaufman et al, 1998;Kerwin et al, 2021). Furthermore, the microbiota of the ANG undergoes a shift during female maturation of the bigfin reef squid (Yang et al, 2021) and Hawaiian bobtail squid (Kerwin et al, 2021). Our previous study further suggested that the environment affects the microbial compositions of individuals, whereas maturity levels exert progressive effects on microbial dynamics over time (Yang et al, 2021).…”

Section: Control Of Mutualistic Microbiota In Squid By Innate Immunitymentioning

confidence: 91%

“…Furthermore, when host immunity is used to regulate symbiont populations, targeted immune responses that recognize symbionts and suppress but do not eliminate symbiont populations should evolve and be maintained. Unlike the single bacterial population hosted in the light organ of the bobtail squid (Ruby and Asato, 1993;Nyholm et al, 2002), a complex microbial consortium is hosted in the ANG of some squid and cuttlefish (Pichon et al, 2005;Collins et al, 2012;Kerwin and Nyholm, 2017;Yang et al, 2021). PPRs/PRMs are detected in light organs and hemocytes of the Hawaiian bobtail squid, including five peptidoglycan recognition proteins (Pgrps, Pgrp1-5), a lipopolysaccharide-binding protein (Lbps, Lbp1), and three bactericidal permeability-increasing proteins (Bpis, Bpi2-4) (Kremer et al, 2013;Krasity et al, 2015).…”

Section: Control Of Mutualistic Microbiota In Squid By Innate Immunitymentioning

confidence: 99%

“…This bacterial consortium within the ANG is populated via horizontal transmission from the marine environment (Kaufman et al, 1998;Li et al, 2019). Although the bacterial consortia of ANGs show variety in different species (Collins et al, 2012;Kerwin and Nyholm, 2017;Yang et al, 2021), its composition shifts gradually during female maturation (Kerwin et al, 2021;Yang et al, 2021). The dynamics of the bacterial consortium in the ANG appear to comprise an intricate process governed by host selection during female maturation.…”

Section: Introductionmentioning

confidence: 99%

“…A single symbiotic bacterial species (V. fischeri) produces bioluminescence in the light organ of the Hawaiian bobtail squid which is controlled by a daily biological rhythm through the quorum-sensing system (Boettcher et al, 1996;Lupp et al, 2003). On the contrary, a complex consortium of microbes synthesizes carotenoids (pigments) which accumulate in the ANG at the time of female maturation (Lum- Kong and Hastings, 1992;Kerwin et al, 2021;Yang et al, 2021). As a result, the color of the ANG changes from white to yellow, orange, and red during sexual maturation in female squids (Bloodgood, 1977;Li et al, 2019;Li et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic profile of symbiotic accessory nidamental gland during female maturation in bigfin reef squid

Tseng

Li²,

Chen³

et al. 2023

Front. Mar. Sci.

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The bigfin reef squid, Sepioteuthis lessoniana, are a valuable commercial species in East Asian regions such as Taiwan and Japan. A lack of genomic information limits the application of potential aquaculture techniques, especially in breeding when considering the hatching rate of offspring. In some squids and cuttlefishes, symbiotic bacteria are transmitted from the accessory nidamental gland (ANG) to the jelly coat of eggs. In Hawaiian bobtail squid, these parent-delivered mutualistic bacteria play an important role in preventing lethal biofouling of the eggs and accelerating the hatch rate of offspring. The bacterial consortium, which is housed in the female squids ANG, are governed by host selection during female maturation. Immune functions are typically used to explain the regulatory mechanism of symbioses by host selection. In this study, we evaluated the transcripts featured in bacterial selection and maintenance during ANG development using RNA-seq. Different developmental stages of ANGs (stages 1–4) were sequenced. The de novo transcriptome assembly resulted in 524,918 unigenes. Two groups, non-pigmentation group (stage 1 and stage 3) and pigmentation group (stage 4), were clustered by transcriptome-wide expression profile analysis. The gene expression analyses indicated that 9,475 differential expression genes (DEGs) in three different phases and 1,363 (14.3%) DEGs were matched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Furthermore, KEGG-enriched analysis results suggested that immune responses are a dominant pathway in the non-pigmentation group (stage 1 and stage 3) whereas lipid metabolism and metabolism of flora fermentation are dominant in the pigmentation group (stage 4). Although the host immunity plays an important role during bacterial colonization of the ANG in bigfin reef squid, our results showed that most immune-related genes had a reduced transcriptomic level in the pigmentation group compared with the non-pigmentation group. Therefore, our results provide new insight to understand the regulatory mechanisms of initial bacterial colonization and later bacterial pigmentation in the bigfin reef squid.

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Section: Control Of Mutualistic Microbiota In Squid By Innate Immunitymentioning

confidence: 80%

Section: Control Of Mutualistic Microbiota In Squid By Innate Immunitymentioning

confidence: 91%

Section: Control Of Mutualistic Microbiota In Squid By Innate Immunitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptomic profile of symbiotic accessory nidamental gland during female maturation in bigfin reef squid

Tseng

Li²,

Chen³

et al. 2023

Front. Mar. Sci.

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Temperature increases induce metabolic adjustments in the early developmental stages of bigfin reef squid (Sepioteuthis lessoniana)

Kuan

You

et al. 2022

Science of The Total Environment

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Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology

Baden

Briseno

Coffing

et al. 2023

Integrative and Comparative Biology

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Few animal groups can claim the level of wonder that cephalopods instill in the minds of researchers and the general public. Much of cephalopod biology, however, remains unexplored: the largest invertebrate brain, difficult husbandry conditions, complex (meta-)genomes, among many other things, have hindered progress in addressing key questions. However, recent technological advancements in sequencing, imaging, and genetic manipulation have opened new avenues for exploring the biology of these extraordinary animals. The cephalopod molecular biology community is thus experiencing a large influx of researchers, emerging from different fields, accelerating the pace of research in this clade. In the first post-pandemic event at the Cephalopod International Advisory Council (CIAC) conference in April 2022, over 40 participants from all over the world met and discussed key challenges and perspectives for current cephalopod molecular biology and evolution. Our particular focus was on the fields of comparative and regulatory genomics, gene manipulation, single cell transcriptomics, metagenomics and microbial interactions. This article is a result of this joint effort, summarizing the latest insights from these emerging fields, their bottlenecks and potential solutions. The article highlights the interdisciplinary nature of the cephalopod -omics community and provides an emphasis on continuous consolidation of efforts and collaboration in this rapidly evolving field.

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Bacterial Dynamics in the Accessory Nidamental Gland of <i>Sepioteuthis lessoniana</i> throughout Maturation

Cited by 5 publications

References 40 publications

Transcriptomic profile of symbiotic accessory nidamental gland during female maturation in bigfin reef squid

Transcriptomic profile of symbiotic accessory nidamental gland during female maturation in bigfin reef squid

Temperature increases induce metabolic adjustments in the early developmental stages of bigfin reef squid (Sepioteuthis lessoniana)

Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology

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