Bacterial Division Proteins FtsZ and ZipA Induce Vesicle Shrinkage and Cell Membrane Invagination

Cabré, Elisa J.; Sánchez-Gorostiaga, Alicia; Carrara, Paolo; Ropero, Noelia; Casanova, Mercedes; Palacios, Pilar; Stano, Pasquale; Jiménez, Mercedes; Rivas, Germán; Vicente, Miguel

doi:10.1074/jbc.m113.491688

Cited by 76 publications

(84 citation statements)

References 52 publications

(54 reference statements)

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“…Procedures to incorporate FtsZ inside giant vesicles, formed by reverse emulsion, with ZipA artificially incorporated at the inner membrane face have recently been optimized (Cabré et al, 2013). These vesicles have been made permeable allowing the control of FtsZ polymerization inside them by externally added ligands that promote assembly, such as GTP and Mg…”

Section: Note Added In Proofmentioning

confidence: 99%

Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell‐like compartments

Jiménez

Martos

Cabré

et al. 2013

Environmental Microbiology

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SummaryThe use of artificial lipid membranes, structured as giant unilamellar vesicles (GUVs), provides the opportunity to investigate membrane-associated biological processes under defined experimental conditions. Due to their large size, they are uniquely adapted to investigate the properties and organization (in time and space) of macromolecular complexes incorporated in the vesicle interior by imaging and micro-spectroscopic techniques. Experimental methods to produce giant vesicles and to encapsulate proteins inside them are here reviewed. Previous experimental work to reconstitute elements of the bacterial division machinery in these membrane-like systems is summarized. Future challenges towards reconstructing minimal divisome assemblies in giant vesicles as cytomimetic containers are discussed.

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Section: Note Added In Proofmentioning

confidence: 99%

Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell‐like compartments

Jiménez

Martos

Cabré

et al. 2013

Environmental Microbiology

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“…Simplified lipid-cytoskeletal protein complexes system have been reconstituted in model membrane systems as GUVS, liposomes and supported lipid membranes (Merkle et al, 2008;Cabré et al, 2013;Osawa and Erickson, 2013;Pontani et al, 2009;MateosGil et al, 2012a) using different ways to anchor the proteins to the membranes. Modeling the segregation of lipid phases under the presence of cytoskeletal proteins is challenging because it is important to include the self-assembling properties of the proteins.…”

Section: Introductionmentioning

confidence: 99%

Modeling the interplay between protein and lipid aggregation in supported membranes

Salas¹,

Encinar²,

Alonso³

et al. 2015

Chemistry and Physics of Lipids

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“…As discussed above in the context of early cell division, a possible minimal divisome architecture inspired from bacterial cytokinesis would consist of FtsZ protofilaments anchored to the liposome membrane by native auxiliary proteins such as the actin homologue FtsA or ZipA (Osawa et al 2008(Osawa et al , 2009Osawa and Erickson 2013;Jimenez et al 2011;Cabre et al 2013). In the absence of a cell wall mimicry, FtsZ assisted by FtsA was suggested as the simplest machinery to recapitulate all essential steps of cellular division in lipid vesicles: Z ring formation, force generation to distort the liposome membrane, progressive constriction of the Z ring and completed division (Osawa and Erickson 2013).…”

Section: Expression Of Membrane Proteins Directly Involved In Cell DImentioning

confidence: 99%

“…Studies using the conventional reconstitution approach based on purified proteins have revealed important insights on the mechanisms of liposome deformation evoked, for instance, by MinE (Shih et al 2011), amphiphysin 1 (Sorre et al 2012), I-BAR domain proteins (Saarikangas et al 2009), clathrin (Dannhauser and Ungewickell 2012), and FtsZ with its associated membrane-binding proteins (Osawa et al 2008;Cabre et al 2013). However, it has not been reported yet that in situ expressed proteins, i.e.…”

Section: Experimental Evidence Of Genetically Controlled Deformation mentioning

confidence: 99%

Toward the assembly of a minimal divisome

2014

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The construction of an irreducible minimal cell having all essential attributes of a living system is one of the biggest challenges facing synthetic biology. One ubiquitous task accomplished by any living systems is the division of the cell envelope. Hence, the assembly of an elementary, albeit sufficient, molecular machinery that supports compartment division, is a crucial step towards the realization of self-reproducing artificial cells. Looking backward to the molecular nature of possible ancestral, supposedly more rudimentary, cell division systems may help to identify a minimal divisome. In light of a possible evolutionary pathway of division mechanisms from simple lipid vesicles toward modern life, we define two approaches for recapitulating division in primitive cells: the membrane deforming protein route and the lipid biosynthesis route. Having identified possible proteins and working mechanisms participating in membrane shape alteration, we then discuss how they could be integrated into the construction framework of a programmable minimal cell relying on gene expression inside liposomes. The protein synthesis using recombinant elements (PURE) system, a reconstituted minimal gene expression system, is conceivably the most versatile synthesis platform. As a first step towards the de novo synthesis of a divisome, we showed that the N-BAR domain protein produced from its gene could assemble onto the outer surface of liposomes and sculpt the membrane into tubular structures. We finally discuss the remaining challenges for building up a self-reproducing minimal cell, in particular the coupling of the division machinery with volume expansion and genome replication.

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Bacterial Division Proteins FtsZ and ZipA Induce Vesicle Shrinkage and Cell Membrane Invagination

Cited by 76 publications

References 52 publications

Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell‐like compartments

Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell‐like compartments

Modeling the interplay between protein and lipid aggregation in supported membranes

Toward the assembly of a minimal divisome

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