Bacterial diversity of the green turtle (Chelonia mydas) nest environment

Candan, Onur; Candan, Esra Deniz

doi:10.1016/j.scitotenv.2020.137717

Cited by 16 publications

(13 citation statements)

References 67 publications

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“…In particular, the bacterial community associated with the eggs of P. unifilis seems to be dominated by Gram-negative aerobic bacteria normally present in the environment, or as part of the turtle microbiota, that may act as opportunistic pathogens under stressful conditions [ 24 , 103 ]. Among such bacteria we found Pseudomonas and Stenotrophomonas , both previously isolated from chelonians [ 24 , 104 , 105 , 106 ]. The genus Pseudomonas is known to be part of the normal microbiota of the mouth and cloaca of several turtles [ 22 , 25 , 103 , 104 , 105 ], including P. unifilis [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…have been also isolated from eggs, and their presence has been linked to low hatching success [ 22 , 23 , 24 , 25 , 104 ]. The only bacterium identified at the species level, Stenotrophomonas maltophilia , has been isolated from several diseased adult animals [ 107 , 108 ], and also from unhatched sea turtle eggs [ 24 , 106 , 107 ]. Notably, infections caused by Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

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Beyond Sea Turtles: Fusarium keratoplasticum in Eggshells of Podocnemis unifilis, a Threatened Amazonian Freshwater Turtle

García-Martín

Sarmiento-Ramírez

Diéguez-Uribeondo

2021

JoF

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The endangered yellow-spotted river turtle (Podocnemis unifilis) has experienced a dramatic population decline in the Ecuadorian Amazonia, mainly due to overexploitation of its eggs. To reverse this trend, the Wildlife Conservation Society has developed a head-start program in Yasuní National Park since 2008, but the potential risk that microbes associated with its eggs might represent for hatching success has not been evaluated yet. Members of the Fusarium solani species complex (FSSC) are involved in egg failure in sea turtles under natural and hatchery conditions, but their role in infecting the eggs of P. unifilis is unknown. In this study, we collected eggshells of P. unifilis and obtained 50 fungal and bacterial isolates. Some potentially pathogenic fungi of the genera Fusarium, Penicillium and Rhizopus were identified based on molecular data. Most importantly, the sea turtle pathogenic species F. keratoplasticum not only was present, but it was the most frequently found. Conversely, we have also isolated other microorganisms, such as Pseudomonas or Phoma-like species, producing a wide spectrum of antifungal compounds that may have a protective role against fungal diseases. Our survey provides useful information on potential pathogens found in P. unifilis eggshells, upon which the success of conservation programs may depend.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Beyond Sea Turtles: Fusarium keratoplasticum in Eggshells of Podocnemis unifilis, a Threatened Amazonian Freshwater Turtle

García-Martín

Sarmiento-Ramírez

Diéguez-Uribeondo

2021

JoF

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“…It is estimated that up to 90% of all wastewater is discharged, untreated, directly into rivers, lakes or oceans [ 3 ] and, for this reason, AMR determinants could be used as indicators of pollution in marine ecosystems [ 4 ]. In addition, resistant microorganisms can persist in the aquatic environment in reservoir species [ 4 , 5 , 6 ] and some wild marine species, with no history of treatment with antimicrobials, can harbour resistant microbial species responsible for AMR genes recombination [ 4 , 7 , 8 , 9 , 10 ]. According to a recent 20-year study conducted by the French Research Institute for Exploitation of the Sea (Ifremer), the Mediterranean marine water is the most waste-polluted in Europe [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance in Loggerhead Sea Turtles (Caretta caretta): A Comparison between Clinical and Commensal Bacterial Isolates

Trotta

Marinaro

Sposato

et al. 2021

Animals

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Gram negative organisms are frequently isolated from Caretta caretta turtles, which can act as reservoir species for resistant microorganisms in the aquatic environment. C. caretta, which have no history of treatment with antimicrobials, are useful sentinel species for resistant microbes. In this culture-based study, commensal bacteria isolated from oral and cloacal samples of 98 healthy C. caretta were compared to clinical isolates from the wounds of 102 injured animals, in order to investigate the presence of AMR bacteria in free-living loggerheads from the Adriatic Sea. A total of 410 isolates were cultured. Escherichia coli and genera such as Serratia, Moraxella, Kluyvera, Salmonella were isolated only in healthy animals, while Acinetobacter, Enterobacter, Klebsiella and Morganella were isolated only from the wounds of the injured animals. When tested for susceptibility to ampicillin, amoxicillin + clavulanic acid, ceftazidime, cefuroxime, gentamicin, doxycycline, ciprofloxacin and enrofloxacin, the clinical isolates showed highly significant differences in AMR rates vs. commensal isolates for all the drugs tested, except for doxycycline. The detection of high AMR rates in loggerheads is of clinical and microbiological significance since it impacts both the choice of a proper antibiotic therapy and the implementation of conservation programs.

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“…Sand samples were collected and mixed from three different points for each subsection along the nesting site mentioned in previous studies [27]. Sand samples were collected from the top layer of the sand profile (0-30 cm) after the removal of the surface.…”

Section: Sample Collectionmentioning

confidence: 99%

Heavy Metal Tolerance Potential of Aspergillus alliaceus Isolated from a Green Turtle Nesting Site

Candan¹

2021

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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Heavy metals are naturally present in the environment. As a result of human activities and some natural events, mixing of these metals in water, air and soil is one of the most serious global problems. The toxicity of these metals is also a serious global problem due to their accumulation in living things and non-biodegradable characteristics. Increasing heavy metal pollution in aquatic and terrestrial environments requires improvement strategies such as bioremediation to remove these metals from the environment for human and environmental health. In this study, fungal isolation was performed to examine fungi with heavy metal remediation potential using sand samples from Sugözü Beaches where are subjected to intense environmental effects due to human activities including industrial facilities and marine traffic. This beach is also an important nesting area for the green turtle. Aspergillus alliaceus isolated from this beach was identified using primers of internal transcribed spacer 1 and 4. To determine the tolerance of A. alliaceus to iron, zinc, cobalt and copper, the fungus was inoculated into media containing metal at different concentrations (200, 400, 600, 800, and 1000 ppm). Daily mycelium growths were recorded during a ten day incubation at 30°C. A.alliaceus was found to tolerate Fe and Zn at all concentrations. Besides, it tolerated Co and Cu up to 400 and 600 ppm, respectively. In this study, the tolerance of A. alliaceus to Fe, Zn, and Co was determined for the first time and it is recommended that it can be used efficiently in bioremediation studies.

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Bacterial diversity of the green turtle (Chelonia mydas) nest environment

Cited by 16 publications

References 67 publications

Beyond Sea Turtles: Fusarium keratoplasticum in Eggshells of Podocnemis unifilis, a Threatened Amazonian Freshwater Turtle

Beyond Sea Turtles: Fusarium keratoplasticum in Eggshells of Podocnemis unifilis, a Threatened Amazonian Freshwater Turtle

Antimicrobial Resistance in Loggerhead Sea Turtles (Caretta caretta): A Comparison between Clinical and Commensal Bacterial Isolates

Heavy Metal Tolerance Potential of Aspergillus alliaceus Isolated from a Green Turtle Nesting Site

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