Bacterial diversity of the cultivable fraction of a marine electroactive biofilm

Vandecandelaere, Ilse; Nercessian, Olivier; Faimali, Marco; Segaert, Eveline; Mollica, A.; Achouak, Wafa; Vos, Paul De; Vandamme, Peter

doi:10.1016/j.bioelechem.2009.07.004

Cited by 41 publications

(26 citation statements)

References 32 publications

(36 reference statements)

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“…Shifts of potential, peak amplitudes, and peak potentials were in the ranges of those observed for bacterial strains isolated from seawater biofilms and for reference or clinical strains by use of the same approach (13,50,51). The strains shown to be able to catalyze the electrochemical reduction of oxygen not only belonged to different phylogenetic groups but also were Gram, catalase, and oxidase positive or negative for all three, confirming recent outcomes (13,62). Different mechanisms have been proposed to explain microbially catalyzed reductions: direct catalysis by adsorbed enzymes such as catalase (32), indirect catalysis through the production of hydrogen hydroxide (16), and production of manganese oxides/hydroxides by manganese-oxidizing bacteria (14).…”

Section: Discussionsupporting

confidence: 74%

Electroactivity of Phototrophic River Biofilms and Constitutive Cultivable Bacteria

Lyautey

Cournet

Morin

et al. 2011

Appl Environ Microbiol

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Electroactivity is a property of microorganisms assembled in biofilms that has been highlighted in a variety of environments. This characteristic was assessed for phototrophic river biofilms at the community scale and at the bacterial population scale. At the community scale, electroactivity was evaluated on stainless steel and copper alloy coupons used both as biofilm colonization supports and as working electrodes. At the population scale, the ability of environmental bacterial strains to catalyze oxygen reduction was assessed by cyclic voltammetry. Our data demonstrate that phototrophic river biofilm development on the electrodes, measured by dry mass and chlorophyll a content, resulted in significant increases of the recorded potentials, with potentials of up to ؉120 mV/saturated calomel electrode (SCE) on stainless steel electrodes and ؉60 mV/SCE on copper electrodes. Thirty-two bacterial strains isolated from natural phototrophic river biofilms were tested by cyclic voltammetry. Twenty-five were able to catalyze oxygen reduction, with shifts of potential ranging from 0.06 to 0.23 V, cathodic peak potentials ranging from ؊0.36 to ؊0.76 V/SCE, and peak amplitudes ranging from ؊9.5 to ؊19.4 A. These isolates were diversified phylogenetically (Actinobacteria, Firmicutes, Bacteroidetes, and Alpha-, Beta-, and Gammaproteobacteria) and exhibited various phenotypic properties (Gram stain, oxidase, and catalase characteristics). These data suggest that phototrophic river biofilm communities and/or most of their constitutive bacterial populations present the ability to promote electronic exchange with a metallic electrode, supporting the following possibilities: (i) development of electrochemistry-based sensors allowing in situ phototrophic river biofilm detection and (ii) production of microbial fuel cell inocula under oligotrophic conditions.

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Section: Discussionsupporting

confidence: 74%

Electroactivity of Phototrophic River Biofilms and Constitutive Cultivable Bacteria

Lyautey

Cournet

Morin

et al. 2011

Appl Environ Microbiol

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“…While Marinobacter spp. are capable of cathode oxidation (21, 22, 35), isolates of Marinobacter sp. and Labrenzia sp.…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomics Supports the Mechanism for Biocathode Electroautotrophy by “CandidatusTenderia electrophaga”

et al. 2017

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Bacteria that directly use electrodes as metabolic electron donors (biocathodes) have been proposed for applications ranging from microbial electrosynthesis to advanced bioelectronics for cellular communication with machines. However, just as we understand very little about oxidation of analogous natural insoluble electron donors, such as iron oxide, the organisms and extracellular electron transfer (EET) pathways underlying the electrode-cell direct electron transfer processes are almost completely unknown. Biocathodes are a stable biofilm cultivation platform to interrogate both the rate and mechanism of EET using electrochemistry and to study the electroautotrophic organisms that catalyze these reactions. Here we provide new evidence supporting the hypothesis that the uncultured bacterium “Candidatus Tenderia electrophaga” directly couples extracellular electron transfer to CO2 fixation. Our results provide insight into developing biocathode technology, such as microbial electrosynthesis, as well as advancing our understanding of chemolithoautotrophy.

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“…A wide diversity of Gram‐positive as well as Gram‐negative microorganisms can promote oxygen reduction at the cathode (Cordas et al ., 2008; Rabaey et al ., 2008; Parot et al ., 2009; Vandecandelaere et al ., 2009; Cournet et al ., 2010; Erable et al ., 2010). The mechanisms for this are as yet unknown and a microorganism that can conserve energy to support growth from oxygen reduction with an electrode serving as the sole electron donor has yet to be described.…”

Section: Oxygen Reductionmentioning

confidence: 99%

Powering microbes with electricity: direct electron transfer from electrodes to microbes

Lovley¹

2010

Environ Microbiol Rep

352

179

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The discovery of electrotrophs, microorganisms that can directly accept electrons from electrodes for the reduction of terminal electron acceptors, has spurred the investigation of a wide range of potential applications. To date, only a handful of pure cultures have been shown to be capable of electrotrophy, but this process has also been inferred in many studies with undefined consortia. Potential electron acceptors include: carbon dioxide, nitrate, metals, chlorinated compounds, organic acids, protons and oxygen. Direct electron transfer from electrodes to cells has many advantages over indirect electrical stimulation of microbial metabolism via electron shuttles or hydrogen production. Supplying electrons with electrodes for the bioremediation of chlorinated compounds, nitrate or toxic metals may be preferable to adding organic electron donors or hydrogen to the subsurface or bioreactors. The most transformative application of electrotrophy may be microbial electrosynthesis in which carbon dioxide and water are converted to multi-carbon organic compounds that are released extracellularly. Coupling photovoltaic technology with microbial electrosynthesis represents a novel photosynthesis strategy that avoids many of the drawbacks of biomass-based strategies for the production of transportation fuels and other organic chemicals. The mechanisms for direct electron transfer from electrodes to microorganisms warrant further investigation in order to optimize envisioned applications.

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Bacterial diversity of the cultivable fraction of a marine electroactive biofilm

Cited by 41 publications

References 32 publications

Electroactivity of Phototrophic River Biofilms and Constitutive Cultivable Bacteria

Electroactivity of Phototrophic River Biofilms and Constitutive Cultivable Bacteria

Metatranscriptomics Supports the Mechanism for Biocathode Electroautotrophy by “CandidatusTenderia electrophaga”

Powering microbes with electricity: direct electron transfer from electrodes to microbes

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