Bacterial diversity of Grenache and Carignan grape surface from different vineyards at Priorat wine region (Catalonia, Spain)

Guisado, María del Carmen Portillo; Franquès, Judit; Araque, Isabel; Reguant, Cristina; Bordons, Albert

doi:10.1016/j.ijfoodmicro.2015.12.002

Cited by 111 publications

(108 citation statements)

References 36 publications

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“…Grapevine bacteria play a key role not only in plant health, but also in crop quality and yields, which can influence the winemaking process (Verginer et al, 2010;Nisiotou et al, 2011). According to Portillo et al (2015) the diversity of epiphytic bacteria on grape berries remains poorly described. Numerous studies have analysed the presence of yeast on the surface of grapes (de Andrés-de Prado et al, 2007;Fleet, 1990) and many have indicated that Saccharomyces cerevisiae is present only in very small numbers on healthy grapes (Martini, 1993;Pretorius, 2000).…”

Section: Discussionmentioning

confidence: 99%

Microorganisms of Grape Berries

Kántor

Mareček

Ivanišová

et al. 2017

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Section: Discussionmentioning

confidence: 99%

Microorganisms of Grape Berries

Kántor

Mareček

Ivanišová

et al. 2017

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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“…The 9 hypervariable regions (V1–V9) of bacteria have all been targeted for the estimation of vineyard bacterial diversity (Leveau and Tech, 2011; Campisano et al, 2014; Perazzolli et al, 2014; Bokulich et al, 2015, 2016a; Burns et al, 2015; Calleja-Cervantes et al, 2015; Piao et al, 2015; Pinto et al, 2015; Zarraonaindia et al, 2015; Holland et al, 2016; Marzano et al, 2016; Portillo et al, 2016). Depending on the region sequenced the data might be similar or differ significantly.…”

Section: Target Genesmentioning

confidence: 99%

“…Furthermore, soil amendments such as fertilizer and/or compost applications can alter the relative abundances of bacterial groups (Calleja-Cervantes et al, 2015). High-throughput analysis of the grapevine phyllosphere, flowers and grape berry surface, demonstrated that the bacterial communities were predominated by Proteobacteria followed by Firmicutes, Actinobacteria, Acidobacteria , and Bacteroidetes (Perazzolli et al, 2014; Pinto et al, 2014, 2015; Portillo and Mas, 2016; Portillo et al, 2016). The relative abundances of the groups vary depending on the plant tissue or organ.…”

Section: Bacterial Communitiesmentioning

confidence: 99%

“…The relative abundances of the groups vary depending on the plant tissue or organ. Dominant taxa include members of the genera Pseudomonas, Sphingomonas, Frigoribacterium, Curtobacterium, Bacillus, Enterobacter, Acinetobacter, Erwinia, Citrobacter, Pantoea , and Methylobacterium (Bokulich et al, 2014, 2016a; Perazzolli et al, 2014; Pinto et al, 2015; Zarraonaindia et al, 2015; Kecskeméti et al, 2016; Portillo and Mas, 2016; Portillo et al, 2016). In contrast, the endophytic community in grape berries mainly comprise Ralstonia, Burkholderia, Pseudomonas, Staphylococcus, Mesorhizobium, Propionibacterium, Dyella , and Bacillus species (Campisano et al, 2014).…”

Section: Bacterial Communitiesmentioning

confidence: 99%

“…Interestingly a low abundance of LAB is often reported with amplicon sequencing phylogenetic surveys (Bokulich et al, 2012; Pinto et al, 2014, 2015). Most importantly, Oenococcus oeni seems to be rarely encountered in grape must except in one study where it was found to be dominant in fermentations of Grenache and Carignan grapes (Portillo et al, 2016). However, several studies show that the levels of this species increase during malolactic fermentation and that in fact it is in most cases the dominant taxa (Marzano et al, 2016; Portillo et al, 2016).…”

Section: Bacterial Communitiesmentioning

confidence: 99%

See 2 more Smart Citations

The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing

2017

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From the time when microbial activity in wine fermentation was first demonstrated, the microbial ecology of the vineyard, grape, and wine has been extensively investigated using culture-based methods. However, the last 2 decades have been characterized by an important change in the approaches used for microbial examination, due to the introduction of DNA-based community fingerprinting methods such as DGGE, SSCP, T-RFLP, and ARISA. These approaches allowed for the exploration of microbial community structures without the need to cultivate, and have been extensively applied to decipher the microbial populations associated with the grapevine as well as the microbial dynamics throughout grape berry ripening and wine fermentation. These techniques are well-established for the rapid more sensitive profiling of microbial communities; however, they often do not provide direct taxonomic information and possess limited ability to detect the presence of rare taxa and taxa with low abundance. Consequently, the past 5 years have seen an upsurge in the application of high-throughput sequencing methods for the in-depth assessment of the grapevine and wine microbiome. Although a relatively new approach in wine sciences, these methods reveal a considerably greater diversity than previously reported, and identified several species that had not yet been reported. The aim of the current review is to highlight the contribution of high-throughput next generation sequencing and metagenomics approaches to vineyard microbial ecology especially unraveling the influence of vineyard management practices on microbial diversity.

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Evaluating the Cytometric Detection and Enumeration of the Wine Bacterium, Oenococcus oeni

2020

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Flow cytometry is a high-throughput tool for determining microbial abundance in a range of medical, environmental, and food-related samples. For wine, determining the abundance of Saccharomyces cerevisiae is well-defined and reliable. However, for the most common wine bacterium, Oenococcus oeni, using flow cytometry to determine cell concentration poses some challenges. O. oeni most often occurs in doublets or chains of varying lengths that can be greater than seven cells. This wine bacterium is also small, at 0.2-0.6 μm and may exhibit a range of morphologies including binary fission and aggregated complexes. This work demonstrates a straightforward approach to determining the suitability of flow cytometry for the chain-forming bacteria, O. oeni, and considerations when using flow cytometry for the enumeration of small microorganisms (<0.5 μm).

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Bacterial diversity of Grenache and Carignan grape surface from different vineyards at Priorat wine region (Catalonia, Spain)

Cited by 111 publications

References 36 publications

Microorganisms of Grape Berries

Microorganisms of Grape Berries

The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing

Evaluating the Cytometric Detection and Enumeration of the Wine Bacterium, Oenococcus oeni

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