Bacterial diversity associated with a newly described bioeroding sponge, Cliona thomasi, from the coral reefs on the West Coast of India

Mote, Sambhaji; Gupta, Vishal; De, Kalyan; Nanajкar, Mandar; Damare, Samir; Ingole, Baban

doi:10.1007/s12223-020-00830-4

Cited by 3 publications

(4 citation statements)

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“…The microbial structure composition of C. varians‐ associated prokaryotes described here was consistent with the published data by Sacristán‐Soriano et al (2020) of C. varians specimens 1.700 km far, from inshore and offshore specimens in coral reef colonies in the Florida Keys, Northern Caribbean Sea. Likewise, the results are sustained by other Cliona species, where Proteobacteria is the dominant phylum (Easson et al, 2020; Ho et al, 2021; Jeong et al, 2015; Mote et al, 2021; Ramsby et al, 2018), indicating that a fraction of the core microbiome is vertically transferred and maintained in this species independent of its geographical location, while also there are signatures and components linked and horizontally transferred between the environmental microbiome and the sponge. Although the class of Alphaproteobacteria was also dominant in C. varians microbiota as in C. orientalis , in C. varians , by contrast, Gammaproteobacteria was the following more abundant class (Ramsby et al, 2018).…”

Section: Discussionmentioning

confidence: 91%

“…Microbiome association and functions in coral bioeroding sponges are the least studied (Mote et al, 2021). The reports available for microbial diversity analyses have been done in Cliona viridis from the Mediterranean (Blanquer et al, 2013), C. varians from the Florida keys (Sacristán-Soriano et al, 2020) and C. orientalis from the Great Barrier Reef (Pineda et al, 2016(Pineda et al, , 2017Ramsby et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Microbiome composition of the marine sponge Cliona varians at the neotropical southern Caribbean Sea displays a predominant core of Rhizobiales and Nitrosopumilaceae

Sánchez-Suárez

Díaz

Junca

et al. 2022

Journal of Applied Microbiology

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Aims: This work aims to characterize the microbial diversity of the encrusting sponge Cliona varians, a pore-forming and coral reef bioeroding marine sponge of emerging spread related to ocean acidification. Methods and results:We analysed the microbiome composition by 16S V4 amplicon next-generation sequencing in a community of the bioeroding coral reef encrusting/excavating marine sponge Cliona varians thriving at the Southern Caribbean Sea. About 87.21% and 6.76% of the sequences retrieved were assigned to the domain Bacteria and Archaea. The most predominant operational taxonomic units were classified as members of the order Rhizobiales and family Nitrosopumilaceae, representing members of not yet characterized genera. Features found strictly conserved in the strain/genomic representatives reported in those microbial taxa are nitrogen fixation and transformation. Conclusion:Our results suggest, in accordance with recent results, that these microbiome members and associated functions could be contributing to the biological fitness of the sponge to be able to colonize and bioerode in environments with low access and scarce availability of nitrogen sources. Significance and Impact of Study: Coral reefs bioresources such as sponge holobionts are intriguing and complex ecosystem units. This study contributes to the knowledge of how C. varians microbiota is composed or shaped, which is crucial to understand its ecological functions.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Microbiome composition of the marine sponge Cliona varians at the neotropical southern Caribbean Sea displays a predominant core of Rhizobiales and Nitrosopumilaceae

Sánchez-Suárez

Díaz

Junca

et al. 2022

Journal of Applied Microbiology

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“…Interestingly, C. patera supported distinct symbiont communities within the cup and stem portions of the sponge. While this is the first study examining the microbiome of C. patera , the microbiomes of several Cliona species have been studied, namely Cliona celata , Cliona delitrix , Cliona lobata , Cliona orientalis , Cliona thomasi , Cliona tumula , Cliona varians , and Cliona viridis ( Jeong et al, 2015 ; Thomas et al, 2016 ; Ramsby et al, 2018 ; Easson et al, 2020 ; Meenatchi et al, 2020 ; Mote et al, 2020 ; Sacristán-Soriano et al, 2020 ). This is, however, the first study of a Clionid sponge to identify distinct microbiomes separated into different sections of an individual sponge.…”

Section: Discussionmentioning

confidence: 99%

Assessing the Diversity and Biomedical Potential of Microbes Associated With the Neptune’s Cup Sponge, Cliona patera

Katermeran

Deignan

et al. 2021

Front. Microbiol.

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Marine sponges are known to host a complex microbial consortium that is essential to the health and resilience of these benthic invertebrates. These sponge-associated microbes are also an important source of therapeutic agents. The Neptune’s Cup sponge, Cliona patera, once believed to be extinct, was rediscovered off the southern coast of Singapore in 2011. The chance discovery of this sponge presented an opportunity to characterize the prokaryotic community of C. patera. Sponge tissue samples were collected from the inner cup, outer cup and stem of C. patera for 16S rRNA amplicon sequencing. C. patera hosted 5,222 distinct OTUs, spanning 26 bacterial phyla, and 74 bacterial classes. The bacterial phylum Proteobacteria, particularly classes Gammaproteobacteria and Alphaproteobacteria, dominated the sponge microbiome. Interestingly, the prokaryotic community structure differed significantly between the cup and stem of C. patera, suggesting that within C. patera there are distinct microenvironments. Moreover, the cup of C. patera had lower diversity and evenness as compared to the stem. Quorum sensing inhibitory (QSI) activities of selected sponge-associated marine bacteria were evaluated and their organic extracts profiled using the MS-based molecular networking platform. Of the 110 distinct marine bacterial strains isolated from sponge samples using culture-dependent methods, about 30% showed quorum sensing inhibitory activity. Preliminary identification of selected QSI active bacterial strains revealed that they belong mostly to classes Alphaproteobacteria and Bacilli. Annotation of the MS/MS molecular networkings of these QSI active organic extracts revealed diverse classes of natural products, including aromatic polyketides, siderophores, pyrrolidine derivatives, indole alkaloids, diketopiperazines, and pyrone derivatives. Moreover, potential novel compounds were detected in several strains as revealed by unique molecular families present in the molecular networks. Further research is required to determine the temporal stability of the microbiome of the host sponge, as well as mining of associated bacteria for novel QS inhibitors.

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“…Within the coastal zone there are a number of critical habitats, including estuaries, lagoons, mangroves, coral reefs, seagrass beds, tidal mudflats, backwaters, salt marshes, rocky coasts, sandy stretches and oceanic islands [45], which harbour a rich diversity of biota, including coral reefs [46] and mangrove forests [47]. However, studies on the microbial diversity of this region are scarce [8, 48].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic diversity and activity screening of cultivable Actinobacteria isolated from marine sponges and associated environments from the western coast of India

Baig

Dahanukar

Shintre

et al. 2021

Access Microbiology

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The phylogenetic diversity of cultivable actinobacteria isolated from sponges (Haliclona spp.) and associated intertidal zone environments along the northern parts of the western coast of India were studied using 16S rRNA gene sequences. A subset of randomly selected actinobacterial cultures were screened for three activities, namely predatory behaviour, antibacterial activity and enzyme inhibition. We recovered 237 isolates from the phylum Actinobacteria belonging to 19 families and 28 genera, which could be attributed to 95 putative species using maximum-likelihood partition and 100 putative species using Bayesian partition in Poisson tree processes. Although the trends in the discovery of actinobacterial genera isolated from sponges were consistent with previous studies from different study areas, we provide the first report of nine actinobacterial species from sponges. We observed widespread non-obligate epibiotic predatory behaviour in eight actinobacterial genera and we provide the first report of predatory activity in Brevibacterium , Glutamicibacter , Micromonospora , Nocardiopsis , Rhodococcus and Rothia . Sponge-associated actinobacteria showed significantly more predatory behaviour than environmental isolates. While antibacterial activity by actinobacterial isolates mainly affected Gram-positive target bacteria with little or no effect on Gram-negative bacteria, predation targeted both Gram-positive and Gram-negative prey with equal propensity. Actinobacterial isolates from both sponges and associated environments produced inhibitors of serine proteases and angiotensin-converting enzyme. Predatory behaviour was strongly associated with inhibition of trypsin and chymotrypsin. Our study suggests that the sponges and associated environments of the western coast of India are rich in actinobacterial diversity, with widespread predatory activity, antibacterial activity and production of enzyme inhibitors. Understanding the diversity and associations among various actinobacterial activities – with each other and the source of isolation – can provide new insights into marine microbial ecology and provide opportunities to isolate novel therapeutic agents.

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Bacterial diversity associated with a newly described bioeroding sponge, Cliona thomasi, from the coral reefs on the West Coast of India

Cited by 3 publications

References 71 publications

Microbiome composition of the marine sponge Cliona varians at the neotropical southern Caribbean Sea displays a predominant core of Rhizobiales and Nitrosopumilaceae

Microbiome composition of the marine sponge Cliona varians at the neotropical southern Caribbean Sea displays a predominant core of Rhizobiales and Nitrosopumilaceae

Assessing the Diversity and Biomedical Potential of Microbes Associated With the Neptune’s Cup Sponge, Cliona patera

Phylogenetic diversity and activity screening of cultivable Actinobacteria isolated from marine sponges and associated environments from the western coast of India

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