2013
DOI: 10.1021/ef400214h
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Bacterial Diversity Analysis of Coal Mine Drainage Enriched by Different Energy Sources

Abstract: Coal mine drainage (CMD) collected from Meitanba mine (Hunan province, China) was studied. Coal, sulfur, ferrous sulfate, and a mixture of sulfur and ferrous sulfate were employed as selective energy substrates to culture CMD cells, and the bacterial biodiversities of each group were investigated. Four groups, C group [coal (15%, m/v) + CMD], S group [sulfur (10%, m/v) + CMD], SF group [sulfur (5%, m/v) + ferrous sulfate (22.35%, m/v) + CMD], and F group [ferrous sulfate (44.7%, m/v) + CMD] were set up. A tota… Show more

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Cited by 5 publications
(4 citation statements)
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References 27 publications
(19 reference statements)
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“…The 9K medium was used for cultivation of A. caldus and A. thiooxidans, which contained 3.0 g of (NH 4 ) 2 SO 4 , 0.1 g of KCl, 0.5 g of K 2 HPO 4 , 0.5 g of MgSO 4 , 0.01 g of Ca(NO 3 ) 2 , and 1 L of deionized water. 17 Sulfur sources, elemental sulfur, were added to the medium at the concentration of 10 g/L. The final pH value was 2.0.…”
Section: ■ Materials and Methodsmentioning
confidence: 99%
“…The 9K medium was used for cultivation of A. caldus and A. thiooxidans, which contained 3.0 g of (NH 4 ) 2 SO 4 , 0.1 g of KCl, 0.5 g of K 2 HPO 4 , 0.5 g of MgSO 4 , 0.01 g of Ca(NO 3 ) 2 , and 1 L of deionized water. 17 Sulfur sources, elemental sulfur, were added to the medium at the concentration of 10 g/L. The final pH value was 2.0.…”
Section: ■ Materials and Methodsmentioning
confidence: 99%
“…Bacterial diversity of AMD collected from the Meitanba mine, Hunan province, China, was studied by different energy sources including coal, sulfur, ferrous sulfate, and a mixture of sulfur and ferrous sulfate (Yang et al, 2013a). The distribution of the microbial communities of the carbon group was more similar to the sulfur group than the sulfur+ferrous sulfate group.…”
Section: Biologicalmentioning
confidence: 99%
“…There are some desulfurizing bacteria, both organosulfur and inorganosulfur, discovered to be used in coal biodegradation. Examples of inorganosulfur bacteria such as Acidithiobacillus caldus , Acidithiobacillus ferrooxidans , Acidithiobacillus thiooxidans and examples of organosulfur desulfurizing bacteria such as Pseudomonas , Bacillus , and Rhodococcus were found . Dibenzothiophene (DBT) is one of the representatives of organic sulfur in coal .…”
Section: Introductionmentioning
confidence: 99%
“…Examples of inorganosulfur bacteria such as Acidithiobacillus caldus, Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans [10][11][12] and examples of organosulfur desulfurizing bacteria such as Pseudomonas, Bacillus, and Rhodococcus were found. 5,13,14 Dibenzothiophene (DBT) is one of the representatives of organic sulfur in coal. 15 There are two pathways, Kodama and sulfur-specific (4S), 16,17 that microbially degrade organic sulfur when the DBT is used as a model compound.…”
Section: Introductionmentioning
confidence: 99%