Bacterial degradation of low concentrations of phenanthrene and inhibition by naphthalene

Shuttleworth, Kay L.; Cerniglia, Carl E.

doi:10.1007/bf00171574

Cited by 60 publications

(40 citation statements)

References 25 publications

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“…Stringfellow and Aitken [28] also corroborated these findings in their work when they showed that naphthalene and other 2-ring PAHs, 2-methylnaphthalene and 1-methylnaphthalene, strongly interfered with Phe degradation. The inhibition of Phe degradation by naphthalene, even at very low concentrations (5 ppm), has also been reported by Shuttleworth and Cerniglia [29].…”

Section: Concentrations Of Pahs (µG/g)supporting

confidence: 74%

Enhanced Degradation of Benzo[α]Pyrene in Coal Tar Contaminated Soils Using Biodiesel

To¹,

Dm²

2018

J Bioremediat Biodegrad

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The biodegradation of the potent carcinogen, benzo [a]pyrene (BaP), and other priority pollutants was investigated in un-weathered coal tar contaminated soil over a period of 150 days. Results from the laboratory microcosm experiments showed that after 60 days, the concentrations of BaP were significantly reduced by 81%, in the biodiesel amended samples compared to the 26% and 34% depletion in the control and nutrient-only amended microcosms, respectively. The 3-ring PAH anthracene was also almost completely biodegraded in the presence of biodiesel. However, phenanthrene degradation was significantly inhibited in these samples as only 3% reduction occurred as opposed to the 80% depletion observed in the control. A stepwise treatment approach conducted on the coal tar spiked soil also revealed a higher reduction in BaP (98%) in the biodiesel amended microcosms compared to the control (29%) and further enhanced the depletion of phenanthrene by 51% after 60 days of adding biodiesel to soil initially treated with nutrients. Toxicity assays showed that biodiesel amended microcosms stimulated phosphatase enzyme activity and exhibited a lower toxic response to Microtox Vibrio fischeri. Overall, the pattern observed in the removal of the PAHs using biodiesel, suggests the co-metabolic action of ligninolytic fungi, probably via lignin peroxidases, as also evidenced from the visible growth of moulds after 14 days of amendment. The enhanced removal of carcinogenic PAH and the reduced toxicity observed in soil after biodiesel amendment, indicates that this bioremediation technique has potential for full scale field trials.

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Section: Concentrations Of Pahs (µG/g)supporting

confidence: 74%

Enhanced Degradation of Benzo[α]Pyrene in Coal Tar Contaminated Soils Using Biodiesel

To¹,

Dm²

2018

J Bioremediat Biodegrad

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“…Pseudomonas and Ralstonia sequences are common in PAH-contaminated soils (10, 14, 26, 41) and were associated with growth on salicylate and naphthalene in this study. Acidovorax species represented the majority of sequences associated with growth on phenanthrene, and they have also been found at PAH-contaminated sites (14,26,37). It is possible that the sequences detected in this study may only represent a portion of the total community capable of growth on the added compounds.…”

Section: Discussionmentioning

confidence: 86%

“…Although a number of phenanthrene-degrading isolates can also grow on naphthalene (4), many others cannot (4,16,18). Additionally, the presence of naphthalene has been shown to inhibit the growth of some phenanthrene-degrading bacteria, including an Acidovorax isolate (37).…”

Section: Discussionmentioning

confidence: 99%

Stable-Isotope Probing of Bacteria Capable of Degrading Salicylate, Naphthalene, or Phenanthrene in a Bioreactor Treating Contaminated Soil

Singleton

Powell

Sangaiah

et al. 2005

Appl Environ Microbiol

134

126

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[ 13 C 6 ]salicylate, [U-13 C]naphthalene, and [U-13 C]phenanthrene were synthesized and separately added to slurry from a bench-scale, aerobic bioreactor used to treat soil contaminated with polycyclic aromatic hydrocarbons. Incubations were performed for either 2 days (salicylate, naphthalene) or 7 days (naphthalene, phenanthrene). Total DNA was extracted from the incubations, the "heavy" and "light" DNA were separated, and the bacterial populations associated with the heavy fractions were examined by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone libraries. Unlabeled DNA from Escherichia coli K-12 was added to each sample as an internal indicator of separation efficiency. While E. coli was not detected in most analyses of heavy DNA, a low number of E. coli sequences was recovered in the clone libraries associated with the heavy DNA fraction of [ 13 C]phenanthrene incubations. The number of E. coli clones recovered proved useful in determining the relative amount of light DNA contamination of the heavy fraction in that sample. Salicylate-and naphthalene-degrading communities displayed similar DGGE profiles and their clone libraries were composed primarily of sequences belonging to the Pseudomonas and Ralstonia genera. In contrast, heavy DNA from the phenanthrene incubations displayed a markedly different DGGE profile and was composed primarily of sequences related to the Acidovorax genus. There was little difference in the DGGE profiles and types of sequences recovered from 2-and 7-day incubations with naphthalene, so secondary utilization of the 13 C during the incubation did not appear to be an issue in this experiment.

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“…Inhibition may also occur, presumably due to competition for enzymes involved in oxidation or transport, accumulation of by-products resulting in cytotoxicity, and blockage of enzyme induction (66,295,590,564). Determining which mechanism is important in any given situation can be complicated by the presence of metabolites from the different PAHs.…”

Section: Aerobic Pah Metabolismmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,195

640

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Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

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Bacterial degradation of low concentrations of phenanthrene and inhibition by naphthalene

Cited by 60 publications

References 25 publications

Enhanced Degradation of Benzo[α]Pyrene in Coal Tar Contaminated Soils Using Biodiesel

Enhanced Degradation of Benzo[α]Pyrene in Coal Tar Contaminated Soils Using Biodiesel

Stable-Isotope Probing of Bacteria Capable of Degrading Salicylate, Naphthalene, or Phenanthrene in a Bioreactor Treating Contaminated Soil

Recent Advances in Petroleum Microbiology

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