Bacterial Degradation of Chlorophenols: Pathways, Biochemica, and Genetic Aspects

Solyanikova, Inna P.; Golovleva, Ludmila

doi:10.1081/pfc-120035921

Cited by 63 publications

(60 citation statements)

References 71 publications

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“…According to a preliminary GC-MS identification, the likely possibility of the odd peak would be an aromatic material, dimethyl phthalate, or vitamin A aldehyde, which could be a bacterial enzyme released at those conditions. However, it has been pointed out by several studies (Chung et al, 2003;Lu et al, 1996;Reardon et al, 2000;Annadurai et al, 2002;Safont et al, 2012) that the formation of dead end metabolic intermediates or the toxic effect of the substrate on the cells is often associated with a yellowish to brownish colour change, often resulting from involving the meta-pathway for degradation, which is more related to incomplete mineralization (Solyanikova and Golovleva, 2004;Lu et al, 1996). Such a colour change has never been detected in any situation in the present study.…”

Section: Effect Of Temperaturecontrasting

confidence: 45%

Biodegradation of 2, 4 Dichlorophenol

Al-Khalid¹,

El‐Naas²

2017

American Journal of Engineering and Applied Sciences

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Abstract:The present study focuses on the optimization of process parameters of the aerobic biodegradation of 2, 4 Dichlorophenol (2, 4 DCP) by a commercial strain of P. putida immobilized in PVA gel matrix, using design of experiments by Response Surface Methodology (RSM). The degradation rates obtained from these experiments were used to evaluate the effects of the main factors and their interactions during the biodegradation of 2, 4 DCP. An effective quadratic regression model predicting the rate in terms of the main independent variables, namely temperature, initial pH and initial concentration of 2, 4 DCP, was developed. The optimum conditions for DCP degradation were obtained as follows: Temperature 32.6°C, pH 5.0 and initial DCP concentration 70.5 mg L −1 , resulting in a maximum predicted degradation rate of 41.8 mg L. Under these optimized conditions, a degradation rate of 40.1 mg L −1 h −1was experimentally obtained, thus validating the model.

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Section: Effect Of Temperaturecontrasting

confidence: 45%

Biodegradation of 2, 4 Dichlorophenol

Al-Khalid¹,

El‐Naas²

2017

American Journal of Engineering and Applied Sciences

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“…The chemical stability of the C-Cl bond is a major problem, which often causes polychlorinated compounds to be minimally degradable by most environmental micro-organisms [2][3][4]. Some aerobic bacteria metabolize highly chlorinated organic compounds, including polychlorinated biphenyls [5], haloalkanes [6][7][8], chlorobenzenes [9,10] and chlorophenols [11,12]. The removal of the halogen substituent occurs through the action of specific dehalogenases [13,14] or as part of a downstream reaction of Cl-containing metabolic intermediates [15].…”

Section: Introductionmentioning

confidence: 99%

Why are chlorinated pollutants so difficult to degrade aerobically? Redox stress limits 1,3-dichloprop-1-ene metabolism byPseudomonas pavonaceae

Nikel

Pérez‐Pantoja

2013

Phil. Trans. R. Soc. B

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Chlorinated pollutants are hardly biodegradable under oxic conditions, but they can often be metabolized by anaerobic bacteria through organohalide respiration reactions. In an attempt to identify bottlenecks limiting aerobic catabolism of 1,3-dichloroprop-1-ene (1,3-DCP; a widely used organohalide) in Pseudomonas pavonaceae , the possible physiological restrictions for this process were surveyed. Flow cytometry and a bioluminescence reporter of metabolic state revealed that cells treated with 1,3-DCP experienced an intense stress that could be traced to the endogenous production of reactive oxygen species (ROS) during the metabolism of the compound. Cells exposed to 1,3-DCP also manifested increased levels of d -glucose-6- P 1-dehydrogenase activity (G6PDH, an enzyme key to the synthesis of reduced NADPH), observed under both glycolytic and gluconeogenic growth regimes. The increase in G6PDH activity, as well as cellular hydroperoxide levels, correlated with the generation of ROS. Additionally, the high G6PDH activity was paralleled by the accumulation of d -glucose-6- P , suggesting a metabolic flux shift that favours the production of NADPH. Thus, G6PDH and its cognate substrate seem to play an important role in P. pavonaceae under redox stress caused by 1,3-DCP, probably by increasing the rate of NADPH turnover. The data suggest that oxidative stress associated with the biodegradation of 1,3-DCP reflects a significant barrier for the evolution of aerobic pathways for chlorinated compounds, thereby allowing for the emergence of anaerobic counterparts.

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“…However, the biodegradation of many aromatics proceeds through the ortho-cleavage pathway after the formation of catechol because the meta-cleavage results in the formation of dead end metabolites from catechol; the enzyme gets inactivated by the accumulation of a toxic intermediate [38]. As a rule, conversion of catechol does not follow the meta-cleavage pathway, and generally, the ortho-cleavage pathway is required for the complete degradation of many aromatic organics [39].…”

Section: Mechanisms Of Biodegradationmentioning

confidence: 99%

Organic Contaminants in Refinery Wastewater: Characterization and Novel Approaches for Biotreatment

Al-Khalid¹,

El‐Naas²

2018

Recent Insights in Petroleum Science and Engineering

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Addressing major environmental issues, such as water pollution, is essential nowadays in realizing sustainable development. The ever-increasing world population and industrial development have led to the introduction of diferent types of chemicals to the environment, leading to considerable deterioration in environmental quality. A major class of these chemicals is phenolic compounds, which are hazardous pollutants and highly toxic even at low concentrations. In recent years, researchers have realized the importance of extracting new bacterial strains that are efective in treating diferent types of highly contaminated wastewaters at diferent severe conditions. They also focused considerable amount of research on developing new types of reactors that would provide eicient mixing and reduce mass transfer limitations. The aim is to develop and evaluate efective reactor systems and biocatalysts for the biodegradation of major contaminants in petroleum reinery wastewater. This chapter examines the diferent available options for the treatment of reinery wastewater with more focus on novel biotreatment options.

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Bacterial Degradation of Chlorophenols: Pathways, Biochemica, and Genetic Aspects

Cited by 63 publications

References 71 publications

Biodegradation of 2, 4 Dichlorophenol

Biodegradation of 2, 4 Dichlorophenol

Why are chlorinated pollutants so difficult to degrade aerobically? Redox stress limits 1,3-dichloprop-1-ene metabolism byPseudomonas pavonaceae

Organic Contaminants in Refinery Wastewater: Characterization and Novel Approaches for Biotreatment

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