Bacterial decontamination of blood stem cell apheresis products

Unrelated bone marrow as a source of Bacillus cereus causing septic shock

“…[1][2][3][4][5][6][7][8][9] However, a recent study published in this journal, proved the feasibility and efficacy of TBI-VP 16-CY in paediatric patients with different types of acute leukaemias at various stages. 10 Our single-centre experience confirms the safety and efficacy of this conditioning regimen in children with ALL in 2nd CR, demonstrated by the transplantedrelated mortality (TRM) and disease-free survival (DFS) estimations.…”

“…8 Storage of bone marrow on ice, maintaining temperature around 15°C, 9 and in vitro decontamination using pretested antibiotics, should be alternatives to decrease or even eliminate bacterial contamination. 10 Although this is the first report of septic shock due to unrelated bone marrow graft contamination, the clinician must be alert, especially if a long time from collection to infusion is encountered. Moreover, in the event of early signs of bacteremia or shock, antimicrobial therapy must be instituted aggressively to prevent serious consequences.…”

Guidelines for Preventing Infectious Complications among Hematopoietic Cell Transplantation Recipients: A Global Perspective

“…Personnel of donation, collection, or transplantation centers, cell-processing laboratories, and courier services should follow current standards for detecting and preventing extrinsic bacterial and fungal contamination of collected stem cell units at the collection site, during processing and transportation, and at the transplant center [126][127][128][129][130][131][132][133][134][135][136][137][138][139][140][141]. Current, comprehensive discussions of these issues are detailed in the Food and Drug Administration (FDA) ''Good Tissue Practice'' regulations [142], in the European Commission regulations [81], and in international standards established by the professional organizations [76,77,143].…”

Contamination of Bone Marrow Products with an Actinomycete ResemblingMicrobacteriumSpecies and Reinfusion into Autologous Stem Cell and Bone Marrow Transplant Recipients

“…Manipulation during processing, either before or after cryopreservation, may potentially result in bacterial contamination. Bacterial contamination of transplant product has been previously described, and it affects !1%-45% of transplant products [2][3][4][5][6][7]. Depending on the contaminating or-ganism, reinfusion of contaminated product can have serious medical consequences.…”