Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

Zhang, Xiaodong; Peng, Yaqin; Zhao, Jing; Li, Qian; Yu, Xiaojuan; Acevedo‐Rocha, Carlos G.; Li, Aitao

doi:10.1186/s40643-019-0290-4

Cited by 64 publications

(45 citation statements)

References 101 publications

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“…Cytochrome P450 (CYP) enzymes are heme monooxygenases [ 194 ] and named after the absorption band at 450 nm observed after binding of carbon monoxide [ 195 , 196 , 197 ]. They are found across all members of the three life domains, bacteria, archea and eukarya [ 198 , 199 , 200 , 201 , 202 ] and have crucial roles in detoxification of drugs and xenobiotics but also in the anabolism of sterols, fatty acids, eicosanoids, and vitamins [ 203 , 204 ]. CYP enzymes have also been shown to generate, similar to COX and LOX enzymes, prostaglandins and leukotrienes from AA as substrate as well as products from concerted reactions with LOX, like hydroxyeicosatetraenoic acids [ 205 , 206 ].…”

Section: Cellular Ros Sourcesmentioning

confidence: 99%

Functions of ROS in Macrophages and Antimicrobial Immunity

2021

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Reactive oxygen species (ROS) are a chemically defined group of reactive molecules derived from molecular oxygen. ROS are involved in a plethora of processes in cells in all domains of life, ranging from bacteria, plants and animals, including humans. The importance of ROS for macrophage-mediated immunity is unquestioned. Their functions comprise direct antimicrobial activity against bacteria and parasites as well as redox-regulation of immune signaling and induction of inflammasome activation. However, only a few studies have performed in-depth ROS analyses and even fewer have identified the precise redox-regulated target molecules. In this review, we will give a brief introduction to ROS and their sources in macrophages, summarize the versatile roles of ROS in direct and indirect antimicrobial immune defense, and provide an overview of commonly used ROS probes, scavengers and inhibitors.

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Section: Cellular Ros Sourcesmentioning

confidence: 99%

Functions of ROS in Macrophages and Antimicrobial Immunity

2021

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“…Cytochrome P450 catalyses the oxidation of a variety of substrates within the body such as N -palmitoylglycine (NPG) (Zhang et al, 2020 ). Within the enzyme, the BM3Heme domain is responsible for substrate binding but requires the partner redox domain in order to be catalytically competent.…”

Section: Resultsmentioning

confidence: 99%

Assessing the Covalent Attachment and Energy Transfer Capabilities of Upconverting Phosphors With Cofactor Containing Bioactive Enzymes

et al. 2020

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Upconverting phosphors (UCPs) convert multiple low energy photons into higher energy emission via the process of photon upconversion and offer an attractive alternative to organic fluorophores for use as luminescent probes. Examples of biosensors utilizing the apparent energy transfer of UCPs and nanophosphors (UCNPs) with biomolecules have started to appear in the literature but very few exploit the covalent anchoring of the biomolecule to the surface of the UCP to improve the sensitivity of the systems. Here, we demonstrate a robust and versatile method for the covalent attachment of biomolecules to the surface of a variety of UCPs and UCNPs in which the UCPs were capped with functionalized silica in order to provide a surface to covalently conjugate biomolecules with surface-accessible cysteines. Variants of BM3Heme, cytochrome C, glucose oxidase, and glutathione reductase were then attached via maleimide-thiol coupling. BM3Heme, glucose oxidase, and glutathione reductase were shown to retain their activity when coupled to the UCPs potentially opening up opportunities for biosensing applications.

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“…When entering the cells of pro- and eukaryotes, saccharin may be affected by high pH values and by a whole complex of enzymes, including the cytochrome P450 superfamily (CYPs; EC 1.14.14.1), which catalyzes the oxidation of the compounds with aliphatic or aromatic rings [ 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. Given this, it is obvious that the isothiazolidinic ring of o -benzoic sulfimide can undergo chemical and/or enzymatic decyclization.…”

Section: Mechanism Of the Biogenic Transformation Of Saccharinmentioning

confidence: 99%

To a Question on the Mechanism of the Antimicrobial Action of Ortho-Benzoic Sulfimide

Kasap

Grishin

2020

Pharmaceuticals

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The article summarizes and compares data on the properties and biological activity of o-benzoic sulfimide and sulfanilamide compounds. Attention is given to the biochemical conditions under which o-benzoic sulfimide and sulfanilamides have similar activity groups. The results of the experimental and theoretical studies aimed at understanding the molecular organization and biological activity of folic acid and its homologous complexes are analyzed. A hypothesis about the possible mechanisms of the formation of such complexes with the participation of o-benzoic sulfimide is presented. The perspectives for the use of o-benzoic sulfimide and its homologues in biomedicine are evaluated.

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Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

Cited by 64 publications

References 101 publications

Functions of ROS in Macrophages and Antimicrobial Immunity

Functions of ROS in Macrophages and Antimicrobial Immunity

Assessing the Covalent Attachment and Energy Transfer Capabilities of Upconverting Phosphors With Cofactor Containing Bioactive Enzymes

To a Question on the Mechanism of the Antimicrobial Action of Ortho-Benzoic Sulfimide

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