Bacterial Content and Ionic Composition of Sputum in Cystic Fibrosis

Kilbourn, J.P.

doi:10.1016/s0140-6736(78)90111-3

Cited by 45 publications

(26 citation statements)

References 5 publications

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“…Although such a situation is not unique to CF, individuals with this disease harbour high numbers of bacteria in bronchopulmonary plugs that are unevenly distributed through the lung tissue (Baltimore et al, 1989;Worlitzsch et al, 2002). Bacterial colonization is probably promoted by the unique composition of the sputum, which contains mucin, lipids, proteins, amino acids, ions and DNA released by neutrophils in concentrations above the mean of a healthy individual (Barth & Pitt, 1996;Kilbourn, 1978;Sahu & Lynn 1978;Smith et al, 1988). Indeed, a number of studies have provided evidence for a correlation between individual sputum components and the emergence or stabilization of certain P. aeruginosa pheno-types (Govan, 1975;Ohman & Chakrabarty, 1982;Wang et al, 1996;Yoon et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung

Sriramulu

Lünsdorf

Lam

et al. 2005

Journal of Medical Microbiology

325

363

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Section: Introductionmentioning

confidence: 99%

Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung

Sriramulu

Lünsdorf

Lam

et al. 2005

Journal of Medical Microbiology

325

363

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“…Later, LopezVidreiro and Reid [8] concluded that the quantitative changes in mucus composition were secondary to infection rather than a primary abnormality of the disease. Abnormalities were also described in the ionic composition of CF sputum by Kilbourn [9] who correlated an increased calcium concentration with colonisation by Staphylococcus aureus, and an increased magnesium content with the presence of mucoid P aeruginosa [lo].…”

Section: Introductionmentioning

confidence: 99%

The high amino-acid content of sputum from cystic fibrosis patients promotes growth of auxotrophic Pseudomonas aeruginosa

Barth

Pitt

1996

Journal of Medical Microbiology

126

116

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“…In particular, glycine-betaine conjugates are known osmo-protectants for many organisms, while an osmo-protective function has also been attributed to N-acetylglutaminylglutamine amide, glutamate and trehalose in PA cells (34). The betaine aldehyde dehydrogenase (BADH) enzymes produce the osmo-protectant glycine-betaine compound, which must reach extremely high concentrations to exert its protective effect, together with enzymes participating in the catabolism of choline (35).…”

Section: Discussionmentioning

confidence: 99%

“…Expression of the betaine aldehyde: NAD(P) + oxidoreductase enzyme (EC 1.2.1.8, BADH), involved in the second step of choline metabolism plus betaine aldehyde dehydrogenase are induced by choline, even when PA cells are grown in the presence of other carbon and nitrogen sources, such as glucose and ammonium (35). As such, PA-BADH is a potential target for antimicrobial agents: in addition to its involvement in choline catabolism, this enzyme may be crucial in bacterial defense against osmotic and oxidative stress, which both occur in infected tissues (37).…”

Section: Discussionmentioning

confidence: 99%

Live-cell high resolution magic angle spinning magnetic resonance spectroscopy for in vivo analysis of Pseudomonas aeruginosa metabolomics

et al. 2013

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Abstract. Pseudomonas aeruginosa (PA) is a pathogenic gram-negative bacterium that is widespread in nature, inhabiting soil, water, plants and animals. PA is a prevalent cause of deleterious human infections, particularly in patients whose host defense mechanisms have been compromised. Metabolomics is an important tool used to study host-pathogen interactions and to identify novel therapeutic targets and corresponding compounds. The aim of the present study was to report the metabolic profile of live PA bacteria using in vivo high-resolution magic angle spinning (HRMAS) nuclear magnetic resonance spectroscopy (NMR), in combination with 1-and 2-dimensional HRMAS NMR. This methodology provides a new and powerful technique to rapidly interrogate the metabolome of intact bacterial cells and has several advantages over traditional techniques that identify metabolome components from disrupted cells. Furthermore, application of multidimensional HRMAS NMR, in combination with the novel technique total through-Bond correlation Spectroscopy (TOBSY), is a promising approach that may be used to obtain in vivo metabolomics information from intact live bacterial cells and can mediate such analyses in a short period of time. Moreover, HRMAS 1 H NMR enables the investigation of the associations between metabolites and cell processes. In the present study, we detected and quantified several informative metabolic molecules in live PA cells, including N-acetyl, betaine, citrulline, alanine and glycine, which are important in peptidoglycan synthesis. The results provided a complete metabolic profile of PA for future studies of PA clinical isolates and mutants. In addition, this in vivo NMR biomedical approach might have clinical utility and should prove useful in gene function validation, the study of pathogenetic mechanisms, the classification of microbial strains into functional/clinical groups, the testing of anti-bacterial agents and the determination of metabolic profiles of bacterial mutants. IntroductionPseudomonas aeruginosa (PA) is a ubiquitous Gram-negative bacterium that inhabits a wide array of natural environments. This bacterium is of significant clinical interest as it is a multi-antibiotic-resistant human pathogen associated with hospital-acquired infections (1,2) and a major cause of morbidity and mortality in cystic fibrosis (CF) patients. It commonly colonizes the lower respiratory and gastrointestinal tracts and the mucosa and skin of hospital patients. The establishment of chronic PA respiratory infections requires a complex adaptive process that mediates essential physiological changes that allow bacterial cells to survive and persist in the host environment. Bacteria secrete small molecules that act as specific signals to positively regulate specialized processes (3), including the production of virulence factors that mediate pathogenic infection, host colonization and the promotion of interspecies microbial interactions (4).Bacterial cell walls are complex, consisting of integrated macromolecules including c...

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Bacterial Content and Ionic Composition of Sputum in Cystic Fibrosis

Cited by 45 publications

References 5 publications

Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung

Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung

The high amino-acid content of sputum from cystic fibrosis patients promotes growth of auxotrophic Pseudomonas aeruginosa

Live-cell high resolution magic angle spinning magnetic resonance spectroscopy for in vivo analysis of Pseudomonas aeruginosa metabolomics

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