Bacterial contamination and antimicrobial susceptibility patterns of intensive care units medical equipment and inanimate surfaces at Ayder Comprehensive Specialized Hospital, Mekelle, Northern Ethiopia

Darge, Addis; Kahsay, Atsebaha Gebrekidan; Niguse, Selam; Hailekiros, Haftamu; Abdulkader, Mahmud

doi:10.21203/rs.2.10931/v1

Cited by 3 publications

(14 citation statements)

References 10 publications

(18 reference statements)

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“…Besides, susceptibility patterns exhibited by the isolates of Klebsiella sp. to amikacin and chloramphenicol were by and large similar to the results of earlier works [13,19]. Apart from Klebsiella sp., other Gram-negative bacilli such as Citrobacter sp., Salmonella sp., Enterobacter sp., E.coli, and Serratia sp., exhibited appreciable resistance against the same antibiotics.…”

Section: Antibiogramsupporting

confidence: 90%

“…Results revealed that in all the three wards, different patient-care equipments and inanimate objects directly or indirectly associated with patients were heavily contaminated by diverse species of bacterial pathogens. Of the total isolates, Gram-positive cocci were the most dominant as compared to the Gram-negative bacteria and were comparable to a number of studies reported from Ethiopia and Nigeria [11,13,14,19,23]. Nevertheless, in contrary to our results, previous studies reported that Gram-negative bacilli are the predominant isolates in hospital settings [7,18,24].…”

Section: Diversity Of Bacterial Isolatessupporting

confidence: 84%

“…Among the Gram-positive cocci, CNS 46 (52.2%) were the most frequently isolated bacteria from all the samples followed by S. aureus 42 (38.5%). This finding is in consonance with other studies that reported CNS as the major bacteria that colonized on inanimate and therapeutic equipments in hospital setups [11,13,25,26,27]. However, our results are not in agreement with other researchers who reported S. aureus as the most encountered isolates in inanimate and therapeutic equipments [12,14,23].…”

Section: Diversity Of Bacterial Isolatessupporting

confidence: 79%

“…was the most commonly isolated bacteria followed by Klebsiella sp. [13]. The frequency of bacterial isolates such as Citrobacter sp.…”

Section: Diversity Of Bacterial Isolatesmentioning

confidence: 99%

“…As bacterial pathogens are still playing a critical role in HAI in Ethiopia, it is imperative to know the location or institution based etiological and susceptibility profiles. A survey of the literature demonstrated that only a few studies have been done so far in Ethiopia to indicate the magnitude of bacterial contamination in patient-care equipments and inanimate objects [11][12][13][14]. Hitherto, no study pertaining to the bacterial contamination of patient-care equipments and inanimate objects in Arba Minch hospital is done.…”

Section: Introductionmentioning

confidence: 99%

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Magnitude, diversity, and antibiogram of bacteria isolated from the selected wards of Arba Minch hospital, Arba Minch, Ethiopia

Manilal¹

2020

Preprint

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Background: Patient-care equipments and inanimate objects contaminated with bacteria are perilous to patients as well as to others who happen to get exposed, and is a persistent problem in developing countries like Ethiopia, it remains overlooked. Therefore, the present study aims to elucidate the magnitude of contamination, diversity and antimicrobial susceptibility pattern of bacteria associated with patient-care equipments and inanimate objects of selected wards of Arba Minch Hospital (AMH), Ethiopia. Methods: A cross-sectional study on the diversity and drug susceptibility pattern of bacteria isolated from inanimate objects and patient-care equipments of three wards of Arba Minch hospital were done. Samples were inoculated into bacteriological media and were identified by biochemical characterization followed by antimicrobial susceptibility tests.Results: Totally, 109 bacterial isolates were identified. The bacterial isolates were identified as Staphylococcus aureus, Coagulase-negative Staphylococcus (CNS), Acinetobacter sp., Klebsiella sp., Citrobacter sp., Enterobacter sp., Salmonella sp., E. coli, and Serratia sp. The surgical ward displayed the utmost contamination rate. Antibiogram of Gram-positive cocci revealed that S. aureus and CNS manifest higher resistance to both penicillin and trimethoprim-sulfamethoxazole. Regarding the Gram-negative bacilli, isolates of Acinetobacter showed 100% resistance to ceftriaxone and ampicillin. The overall prevalence of multi-drug resistant bacteria in this study was 57.7 %.Conclusionː Results revealed that the surgical ward, followed by paediatric and neonatal intensive care unit of AMH was heavily contaminated. Therefore, a stringent infection vigilance program comprising of routine sampling from the equipments and wards along with antimicrobial resistance surveillance and decontamination efforts must be instituted.

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Section: Antibiogramsupporting

confidence: 90%

Section: Diversity Of Bacterial Isolatessupporting

confidence: 84%

Section: Diversity Of Bacterial Isolatessupporting

confidence: 79%

“…was the most commonly isolated bacteria followed by Klebsiella sp. [13]. The frequency of bacterial isolates such as Citrobacter sp.…”

Section: Diversity Of Bacterial Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnitude, diversity, and antibiogram of bacteria isolated from the selected wards of Arba Minch hospital, Arba Minch, Ethiopia

Manilal¹

2020

Preprint

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Bacterial Contamination of Neonatal Intensive Care Unit

Ashur

2022

KJDMR

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Background and objectives. The environmental impact assessment plays an important role in ensuring environmental media. This study aimed to assess the environmental pollution of the neonatal intensive care unit in Al-Jala hospital of obstetrics and gynecology, Tripoli, Libya through environmental monitoring of bacteria on different surfaces. Methods. A cross-sectional, descriptive study was carried out in the neonatal intensive care unit from December- to February 2022 at Al-Jala hospital, Tripoli. A total of 61 samples were collected with a sterile swab from high-contact environmental surfaces. Isolation, identification, and antibiotic resistance of bacterial isolated were performed by standard technique. Blood culture isolates from neonatal intensive care unit patients were compared with the environmental isolates during the study period. Results. Out of 61 samples, bacterial growth was observed in 58 samples, out of which 40 (68.9%) were gram-positive and 18 (31.03%) were gram-negative. The observed gram-positive bacterial isolate was Staphylococcus aureus 13(32.5%), and most of gram-positive isolates were recovered from an incubator, suction tip, stethoscope, ambu bag, and staff hand. The potential gram-negative bacterial isolates were E. coli 10 (55.5%), and the majority of gram-negative isolates were recovered from the door handle, suction tip, neonate breathing tube, and staff hand. The isolated bacteria were resistant to amoxicillin (41.3%), clindamycin (39.6%), imipenem, and tetracycline (27.5%). Common potential pathogens isolated from the blood culture of NICU patients were staphylococcus aureus. Conclusion. Bacterial contamination of objects and instruments in neonatal intensive care units was high (95%). This study emphasizes the need for rigorous decontamination protocols and hand hygiene. Clindamycin and tetracycline may be used for empirical therapy in clinically suspected cases of isolates.

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Antibiogram and Plasmid Profile of Bacterial Isolates from Intensive Care Units in a Tertiary Healthcare Facility in Umuahia

Okey-kalu

Nwankwo²,

Onyekwere³

et al. 2022

J. Hum. Environ. Health Promot

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Bacterial contamination and antimicrobial susceptibility patterns of intensive care units medical equipment and inanimate surfaces at Ayder Comprehensive Specialized Hospital, Mekelle, Northern Ethiopia

Cited by 3 publications

References 10 publications

Magnitude, diversity, and antibiogram of bacteria isolated from the selected wards of Arba Minch hospital, Arba Minch, Ethiopia

Magnitude, diversity, and antibiogram of bacteria isolated from the selected wards of Arba Minch hospital, Arba Minch, Ethiopia

Bacterial Contamination of Neonatal Intensive Care Unit

Antibiogram and Plasmid Profile of Bacterial Isolates from Intensive Care Units in a Tertiary Healthcare Facility in Umuahia

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