Bacterial complexes of the fruiting bodies and hyphosphere of certain basidiomycetes

Zagriadskaia, Yu. A.; Лысак, Л. В.; Sidorova, Irina; Aleksandrova, A. V.; Voronina, Elena

doi:10.1134/s106235901304016x

Cited by 16 publications

(16 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that the abundance of Proteobacteria is stimulated by higher nutritional status of soil in contrast to, e.g., Acidobacteria which prefer low-nutrient soils (Torsvik and Øvreås, 2002). Thus it is likely that the dominance of Proteobacteria in hyphae (Cho et al, 2003), fruitbodies (Barbieri et al, 2005; Dahm et al, 2005; Zagriadskaia et al, 2013), and mycorrhizal roots (Poole et al, 2001; Frey-Klett et al, 2007) is due to elevated carbon content in these fungal habitats.…”

Section: Discussionmentioning

confidence: 99%

“…Although mushrooms have one of the largest fruitbodies among fungi, potentially harboring highly diverse bacteria, evidence of their microbiome is limited and almost exclusively to taxa that grow in pure culture (Rangel-Castro et al, 2002a; Tsukamoto et al, 2002; Kumari et al, 2013). Only a few studies have analyzed mushrooms from different genera in this regard (Zagriadskaia et al, 2013; de Carvalho et al, 2015). More comprehensive studies on characterizing the bacterial communities using next generation sequencing methods have focused of truffles and similar ascomycetes with belowground fruitbodies (e.g., Antony-Babu et al, 2014; Benucci and Bonito, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, environmental forces that shape bacterial community composition in soil may indirectly contribute to the structure of bacterial communities in fungal mycelia and fruitbodies (Warmink et al, 2009; Antony-Babu et al, 2014). In case of mushrooms, acquisition of bacteria from air has also been suggested (Zagriadskaia et al, 2013). In addition to abiotic factors, host identity has been observed to affect the community composition of mycorrhiza-helper bacteria (Poole et al, 2001; Frey-Klett et al, 2007) as well as that of bacteria in the mycosphere (Warmink et al, 2009) and fungal fruitbodies (Dahm et al, 2005; Kumari et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

2017

View full text Add to dashboard Cite

Despite recent advances in understanding the microbiome of eukaryotes, little is known about microbial communities in fungi. Here we investigate the structure of bacterial communities in mushrooms, including common edible ones, with respect to biotic and abiotic factors in the boreal forest. Using a combination of culture-based and Illumina high-throughput sequencing, we characterized the bacterial communities in fruitbodies of fungi from eight genera spanning four orders of the class Agaricomycetes (Basidiomycota). Our results revealed that soil pH followed by fungal identity are the main determinants of the structure of bacterial communities in mushrooms. While almost half of fruitbody bacteria were also detected from soil, the abundance of several bacterial taxa differed considerably between the two environments. The effect of host identity was significant at the fungal genus and order level and could to some extent be ascribed to the distinct bacterial community of the chanterelle, representing Cantharellales—the earliest diverged group of mushroom-forming basidiomycetes. These data suggest that besides the substantial contribution of soil as a major taxa source of bacterial communities in mushrooms, the structure of these communities is also affected by the identity of the host. Thus, bacteria inhabiting fungal fruitbodies may be non-randomly selected from environment based on their symbiotic functions and/or habitat requirements.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

2017

View full text Add to dashboard Cite

show abstract

“…This amount is compara ble with the content of bacteria in the upper horizon of soddy podzolic soil under mixed forest [6]. The scale of bacteria input with the destructed fungal fragments can be relatively great, taking into account the signifi cant biomass of fungi developed on the soil surface in summer and autumn.…”

Section: Discussionmentioning

confidence: 97%

“…It was shown earlier that the mycelium of basidio mycetes forms specific loci (hyphosphere) in the soil that are differed from surrounding control soil in terms of the population density of bacteria and the structure of saprotrophic bacterial complex [2,5,6,19]. Certain biocenotic relationships between bacterial communi ties of fruit bodies and hyphosphere of basidiomycetes were revealed.…”

Section: Discussionmentioning

confidence: 99%

Bacterial communities in the fruit bodies of ground basidiomycetes

2015

Self Cite

View full text Add to dashboard Cite

Fruit bodies of basidiomycetes at different stages of decomposition serve as specific habitats in for est biocenoses for bacteria and differ significantly with respect to the total bacterial population and abun dance of particular bacterial genera. A significant increase in the total bacterial population estimated by the direct microscopic method with acridine orange staining and in the population of saprotrophic bacteria (inoculation of glucose peptone yeast agar) in fruit bodies of basidiomycetes Armillaria mellea and Coprinus comatus was recorded at the final stage of their decomposition in comparison with the initial stage. Gram negative bacteria predominated in the tissues of fruit bodies at all the stages of decomposition and were rep resented at the final stage by the Aeromonas, Vibrio, and Pseudomonas genera (for fruit bodies of A. mellea) the Pseudomonas genus (for fruit bodies of C. comatus). The potential influence of bacterial communities in the fruit bodies of soil basidiomycetes on the formation of bacterial communities in the upper soil horizons in forest biocenoses is discussed. The loci connected with the development and decomposition of fruit bodies of basidiomycetes on the soil surface are promising for targeted search of Gram negative bacteria, the impor tant objects of biotechnology.

show abstract

Phylum‐level diversity of the microbiome of the extremophilic basidiomycete fungus Pisolithus arhizus (Scop.) Rauschert: An island of biodiversity in a thermal soil desert

et al. 2020

View full text Add to dashboard Cite

We used high‐throughput DNA sequencing methods combined with bio‐geochemical profiles to characterize the internal environment and community structure of the microbiome of the basidiomycete fungus Pisolithus arhizus (Scop.) Rauschert from soils within a geothermal feature of Yellowstone National Park. Pisolithus arhizus is unique in that it forms closed fruiting bodies that sequester visible sulfur within. Fourier transform infrared spectroscopy (FTIR) analysis demonstrates that the P. arhizus fruiting body also concentrates copper, manganese, nickel, and zinc and contains pure granular silica. Gas chromatography‐mass spectrometry (GC‐MS) analysis indicates an environment rich in hydrocarbons. Oxygen probe analysis reveals that zones of up to 4× atmospheric oxygen exist within nanometers of zones of near anoxia. Analysis of microbial community structure using high‐throughput DNA sequencing methods shows that the fruiting body supports a microbiome that reflects the physiochemical environment of the fruiting body. Diversity and richness measures indicate a microbiome that is significantly richer and more diverse than that of the soils in which P. arhizus grows. Further, P. arhizus sporocarps are enriched significantly in Proteobacteria (primarily Burkholderia ) Gemmatimonadetes, Bacteroidetes, Verrucomicrobia, Nitrospirae, Elusimicrobia, and Latescibacteria (WS3) while soils are enriched in Actinobacteria (primarily Mycobacterium ), Dormibacteraeota (AD3), and Eremiobacteraeota (WPS‐2). Finally, pairwise % similarity comparisons indicate that P. arhizus harbors two lineages that may represent new groups in the candidate phylum radiation (CPR). Together, these results demonstrate that P. arhizus provides a novel environment for microbiome studies and provides for interesting hypotheses regarding the evolution, origins, and functions of symbioses and novel microbes.

show abstract

Bacterial complexes of the fruiting bodies and hyphosphere of certain basidiomycetes

Cited by 16 publications

References 7 publications

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

Bacterial communities in the fruit bodies of ground basidiomycetes

Phylum‐level diversity of the microbiome of the extremophilic basidiomycete fungus Pisolithus arhizus (Scop.) Rauschert: An island of biodiversity in a thermal soil desert

Contact Info

Product

Resources

About