Bacterial Community Structure Associated With Elective Cesarean Section Versus Vaginal Delivery in Chinese Newborns

Liu, Dong; Yu, Jialin; Li, Lu‐Quan; Ai, Qing; Feng, Jinxing; Song, Chao; Li, Hongdong

doi:10.1097/mpg.0000000000000606

Cited by 31 publications

(30 citation statements)

References 29 publications

(58 reference statements)

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“…The high standard deviation indicated the occasional occurrence of colonies on MWMQ, which often appeared as hardly visible micro-colonies. Summing up, in 5 of 10 tested infants delivered by caesarean section, bifidobacteria were absent in agreement with findings previously described by Vlkova et al (2005) and Liu et al (2015). According to these reports, such types of infant faecal samples showed higher probability of decreased bifidobacterial counts and increased counts of clostridia.…”

Section: Selectivity Of Mwmq Tested On Faecal Samplessupporting

confidence: 90%

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Anticlostridial agent 8-hydroxyquinoline improves the isolation of faecal bifidobacteria on modified Wilkins-Chalgren agar with mupirocin

Nováková

Vlková

Salmonová

et al. 2016

Lett Appl Microbiol

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Routine isolation of bifidobacteria from mammalian faeces does not use a reliable selective agar with an anticlostridial agent. Overgrowth of clostridia may result in incorrect estimation of bifidobacterial counts. Thus, in order to improve the selectivity of existing media for bifidobacterial isolation, we chose the modified Wilkins-Chalgren agar with mupirocin and supplemented it with 8-hydroxyquinoline (8HQ), a molecule that shows anticlostridial activity without affecting the growth of bifidobacteria. This newly composed medium showed enhanced selectivity and specificity compared to the original medium and therefore, can be recommended for the isolation of bifidobacteria from mammal faeces.

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Section: Selectivity Of Mwmq Tested On Faecal Samplessupporting

confidence: 90%

“…() and Liu et al . (). According to these reports, such types of infant faecal samples showed higher probability of decreased bifidobacterial counts and increased counts of clostridia.…”

Section: Resultsmentioning

confidence: 97%

Anticlostridial agent 8-hydroxyquinoline improves the isolation of faecal bifidobacteria on modified Wilkins-Chalgren agar with mupirocin

Nováková

Vlková

Salmonová

et al. 2016

Lett Appl Microbiol

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“…Birth mode of delivery has been shown to affect the microbiota of breast milk in the majority of studies, suggesting a difference in the milk microbiome between infants born via c-section and those born vaginally ( Azad et al, 2013 ; Gregory et al, 2015 ; Liu et al, 2015 ; Brumbaugh et al, 2016 ; Dominguez-Bello et al, 2016 ; Lee et al, 2016 ; Nagpal et al, 2016 ; Rutayisire et al, 2016 ). In our study, we observed a differential clustering of microbiomes from the breast milk of c-sections versus vaginally delivering mothers.…”

Section: Discussionmentioning

confidence: 99%

Personalization of the Microbiota of Donor Human Milk with Mother’s Own Milk

et al. 2017

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The American Academy of Pediatrics recommends that extremely preterm infants receive mother’s own milk (MOM) when available or pasteurized donor breast milk (DBM) when MOM is unavailable. The goal of this study was to determine whether DBM could be inoculated with MOM from mothers of preterm infants to restore the live microbiota (RM). Culture dependent and culture independent methods were used to analyze the fluctuations in the overall population and microbiome, respectively, of DBM, MOM, and RM samples over time. Using MOM at time = 0 (T0) as the target for the restoration process, this level was reached in the 10% (RM-10) and 30% (RM-30) mixtures after 4 h of incubation at 37°C, whereas, the larger dilutions of 1% (RM-1) and 5% (RM-5) after 8 h. The diversity indexes were similar between MOM and DBM samples, however, different genera were prevalent in each group. Interestingly, 40% of the bacterial families were able to expand in DBM after 4 h of incubation indicating that a large percentage of the bacterial load present in MOM can grow when transferred to DBM, however, no core microbiome was identified. In summary, the microbiome analyses indicated that each mother has a unique microbiota and that live microbial reestablishment of DBM may provide these microbes to individual mothers’ infants. The agreement between the results obtained from the viable bacterial counts and the microbiome analyses indicate that DBM incubated with 10–30% v/v of the MOM for 4 h is a reasonable restoration strategy.

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“…A number of studies have characterized the colonization and development of infant microbiota in western countries111213; however, little is known about this in healthy Chinese infants. Liu D et al 14 used 16S rDNA PCR-DGGE methodology to provide a profile of the dominant gut microbiota of Chinese neonates, but the PCR-DGGE method used may limit the generalizability of their results. It has been previously demonstrated that species richness and diversity were higher for the samples analyzed by 454-pyrosequencing than DGGE15.…”

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confidence: 99%

Composition of gut microbiota in infants in China and global comparison

Kuang

Guo

et al. 2016

Sci Rep

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Symbiotic gut microbiota is essential for human health, and its compositional changes have been associated with various complex disorders. However, systematic investigation of the acquisition and development of gut microbial communities during early infancy are relatively rare, particularly for infants from non-Western countries. In this study, we characterize the colonization and development of infant microbiota in healthy Chinese infants and compare the pattern with those from other countries. The fecal microbiota of 2-month-old infants was considerably more diverse than that of neonates, as indicated by higher relative abundances of Veillonella, Clostridium, Bacteroides, Lactobacillus, Collinsella and Prevotella, and reduction of Escherichia and Enterococcus. The fecal microbiota of vaginally delivered infants (both neonates and 2-month-old) had significant enrichment of Bacteroides, Parabacteroides and Megamonas, whereas cesarean delivered infants had enrichment of Prevotella, Streptococcus and Trabulsiella. By global comparison, we identify three different enterotypes, referred as “P-type”, “A-type ”and “F-type” which were highly abundant in Proteobacteria, Actinobacteria and Firmicutes, respectively. The three enterotypes’ compositons vary geographically. All Chinese infants in our study belong to the P-type. These findings may provide novel insights into our understanding of the establishment of infant fecal bacterial communities.

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Bacterial Community Structure Associated With Elective Cesarean Section Versus Vaginal Delivery in Chinese Newborns

Abstract: These results demonstrate that delivery method has a profound influence on the structure of the intestinal microbiota in Chinese newborn infants. This is in accordance with data reported in other regions.

Cited by 31 publications

References 29 publications

Anticlostridial agent 8-hydroxyquinoline improves the isolation of faecal bifidobacteria on modified Wilkins-Chalgren agar with mupirocin

Anticlostridial agent 8-hydroxyquinoline improves the isolation of faecal bifidobacteria on modified Wilkins-Chalgren agar with mupirocin

Personalization of the Microbiota of Donor Human Milk with Mother’s Own Milk

Composition of gut microbiota in infants in China and global comparison

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