Bacterial community structure and metabolic activity of drinking water pipelines in buildings: A new perspective on dual effects of hydrodynamic stagnation and algal organic matter invasion

Zhang, Haihan; Zhao, Kexin; Liu, Xiang; Chen, Shengnan; Guo, Honghong; Ma, Ben; Yang, Wenbo; Yang, Yansong; Liu, Hanyan

doi:10.1016/j.watres.2022.119161

Cited by 22 publications

(5 citation statements)

References 74 publications

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“…The exception ( Sphingomonas ) is consistent with previous literature, whereby the order Sphingomonadales is a known oxidative degrader of TCP [ 31 ] and a known TCA producer [ 27 ]. The contribution of Sphingomonadales to the microbial conversion of TCP to TCA would be of importance because of a certain dominance of this species in drinking water distribution systems [ 32 ]. Although one isolate out of three Acinetobacter spp.…”

Section: Resultsmentioning

confidence: 99%

Novel and Simple Method for Quantification of 2,4,6-Trichlorophenol with Microbial Conversion to 2,4,6-Trichloroanisole

Goto,

Urase,

Nakakura

2023

Microorganisms

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Contamination with 2,4,6-trichloroanisole (TCA) often causes taste and odor (T&O) problems in drinking water due to its low odor threshold concentration. Microbial O-methylation of the precursor 2,4,6-trichlorophenol (TCP) would be the dominant mechanism for TCA formation. Simple and rapid measurement of TCP in the low concentration range is necessary to control the problems induced by TCA. In this study, the combination of microbial conversion and instrumental analysis was proposed as a method of TCP quantification. Fungi and bacteria were isolated from various water samples and examined for their ability to produce TCA from TCP. As a result, a strain exhibiting quantitative TCA production and a high growth rate was obtained and named Mycolicibacterium sp. CB14. The conversion rate of TCP to TCA by this strain was found to be high and stable (85.9 ± 5.3%), regardless of the applied TCP concentration, although within the range of 0.1–10 µg/L. The limits of detection and quantification for TCP by this proposed method were determined to be 5.2 ng/L and 17.3 ng/L, respectively. By improving the methods, Mycolicibacterium sp. CB14 could be used for the quantification of TCP at very low concentration levels, which is sufficient to manage the T&O problem caused by TCA.

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Section: Resultsmentioning

confidence: 99%

Novel and Simple Method for Quantification of 2,4,6-Trichlorophenol with Microbial Conversion to 2,4,6-Trichloroanisole

Goto,

Urase,

Nakakura

2023

Microorganisms

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“…The aerobic denitrification capacity of strains PYX97 and TSJ96 monocultures and cocultures was investigated under different reaction conditions, including varying carbon/nitrogen ratio (C/N = 1, 4, 7, 10, and 13), pH levels (pH = 5, 6, 7, 8, and 9), and shaking speed (30, 60, 90, 120, and 150 rpm). The shaking speed in the dark shaking biochemical incubator was found to be associated with the dissolved oxygen (DO) concentrations. − The relationship between DO concentrations and the shaking speed was measured: DO 30rpm/30°C = 1.6 mg/L, DO 60rpm/30°C = 3.5 mg/L, DO 90rpm/30°C = 5.2 mg/L, DO 120rpm/30°C = 6.5 mg/L, DO 150rpm/30°C = 8.4 mg/L. − The denitrification performance was evaluated by measuring the nitrate concentrations and the OD 600 values at the end of cultivation.…”

Section: Methodsmentioning

confidence: 99%

“…The phylogenetic trees were constructed by MEGA software . The heatmap of carbon sources metabolic activity map was built using Tbtools software . The network analysis was performed using R software and Gephi software .…”

Section: Methodsmentioning

confidence: 99%

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Aerobic Denitrification Promoting by Actinomycetes Coculture: Investigating Performance, Carbon Source Metabolic Characteristic, and Raw Water Restoration

Ma,

Yang,

et al. 2023

Environ. Sci. Technol.

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The coculture theory that promotes denitrification relies on effectively utilizing the resources of low-efficiency denitrification microbes. Here, the strains Streptomyces sp. PYX97 and Streptomyces sp. TSJ96 were isolated and showed lower denitrification capacity when cultured individually. However, the coculture of strains PYX97 and TSJ96 enhanced nitrogen removal (removed 96.40% of total nitrogen) and organic carbon reduction (removed 92.13% of dissolved organic carbon) under aerobic conditions. Nitrogen balance analysis indicated that coculturing enhanced the efficiency of nitrate converted into gaseous nitrogen reaching 70.42%. Meanwhile, the coculturing promoted the cell metabolism capacity and carbon source metabolic activity. The coculture strains PYX97 and TSJ96 thrived in conditions of C/N = 10, alkalescence, and 150 rpm shaking speed. The coculturing reduced total nitrogen and COD Mn in the raw water treatment by 83.32 and 84.21%, respectively. During this treatment, the cell metabolic activity and cell density increased in the coculture strains PYX97 and TSJ96 reactor. Moreover, the coculture strains could utilize aromatic protein and soluble microbial products during aerobic denitrification processes in raw water treatment. This study suggests that coculturing inefficient actinomycete strains could be a promising approach for treating polluted water bodies.

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“…For each concentration, a parallel group was set, and the concentrations of NO 3 − −N (and Fe(II)) were tested after 24 h. The NO 3 − −N reduction rate and Fe(II) oxidation rate were calculated as described above. The Haldane model was used to fit the data to see the effects of substrate concentration on nitrate reduction rate and Fe(II) oxidation rate [15] (Equation ( 1)):…”

Section: Determination Of the Denitrification Activitymentioning

confidence: 99%

Efficiency Determination and Mechanism Investigation of Autotrophic Denitrification Strain F1 to Promote Low-Carbon Development

Qin

Wan

Liu

et al. 2022

Water

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Shewanella sp. strain F1, isolated from a lab-scale Fe(II)−dependent anaerobic denitrifying reactor, could reduce nitrate by oxidizing Fe(II). Its nitrate reduction rate and Fe(II) oxidation rate were 0.48 mg/(L·h) and 5.05 mg/(L·h) at OD600 of 0.4786 with a five-fold diluent. Shewanella sp. was popular in Fe(III) reduction. Fewer studies about its ability for Fe(II) oxidation are available. The low pH was determined to be the switch for Shewanella sp. strain F1 to perform Fe(III) reduction or Fe(II) oxidation. Even under a low pH, the produced Fe(III) precipitated around cells from iron encrustation. By observation of the morphologies of strain F1, two corresponding microbial mechanisms were proposed. One was named Cyc 2−based Fe(II)-dependent denitrification, in which Fe(II) was oxidized by Cyc 2 in the outer cell membrane, and the produced Fe(III) precipitated on the cell wall surface to form tiled iron encrustation. The other was named Cyc 1−based Fe(II)−dependent denitrification, in which Fe(II) was oxidized on the existing iron precipitation on the cell wall surface to form towery iron encrustation, and the electron was transported to Cyc 1 in the periplasm. The efficiency determination and mechanism investigation of strain F1 will promote the development of autotrophic denitrification technology and meet the requirement of a low−carbon policy.

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Bacterial community structure and metabolic activity of drinking water pipelines in buildings: A new perspective on dual effects of hydrodynamic stagnation and algal organic matter invasion

Cited by 22 publications

References 74 publications

Novel and Simple Method for Quantification of 2,4,6-Trichlorophenol with Microbial Conversion to 2,4,6-Trichloroanisole

Novel and Simple Method for Quantification of 2,4,6-Trichlorophenol with Microbial Conversion to 2,4,6-Trichloroanisole

Aerobic Denitrification Promoting by Actinomycetes Coculture: Investigating Performance, Carbon Source Metabolic Characteristic, and Raw Water Restoration

Efficiency Determination and Mechanism Investigation of Autotrophic Denitrification Strain F1 to Promote Low-Carbon Development

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