Bacterial Community Structure and Diversity of Closely Located Coastal Areas

Haider, Md. Nurul; Nishimura, Masahiko; Kogure, Kazuhiro

doi:10.4236/ojms.2016.63036

Cited by 4 publications

(3 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 ). Seasonal changes influenced the physicochemical parameters of different water bodies, resulting in qualitative and quantitative changes in bacterial populations [ 8 – 11 ]. In the case of fishponds, a similar trend in the fluctuation of bacterial abundance was reported [ 6 , 12 , 13 , 21 ], which ultimately influences the bacterial flora of the cultured fish species [ 12 , 14 – 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…In general, the physicochemical parameters of water, especially the temperature, pH, and transparency, show seasonal fluctuations. Seasonal changes are recognized as the most critical factor causing qualitative and quantitative changes in bacterial populations in various bodies of water [ 8 – 11 ]. A similar trend of bacterial fluctuations was reported in numerous studies on cultured ponds [ 6 , 12 , 13 ] and cultured fish species [ 12 , 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Viable bacterial counts of the Pangasius catfish (Pangasianodon hypophthalmus), their responses to seasonal variations of physicochemical parameters, and bacterial counts of the cultured ponds

Haider¹,

Islam²,

Mukit³

et al. 2022

J Adv Vet Anim Res

View full text Add to dashboard Cite

Objectives: The study was undertaken to evaluate the influences of some physicochemical param¬eters and viable bacterial counts in cultured ponds (water and sediment) on the viable counts of the Pangasius catfish (Pangasianodon hypophthalmus) (gill and intestine) on a seasonal scale. Materials and Methods: Physicochemical parameters, viz., ambient temperature, water tem¬perature, water transparency, pH of the pond waters, and viable bacterial counts of pond water, sediment, fish gills, and intestines, were monitored during four different seasons. The responses of viable counts of bacteria to the seasonal changes of physicochemical parameters were also assessed using redundancy analysis (RDA) and a heatmap coupled with the clustering analysis. Results: Except for fluctuations in air and water temperatures, the other two physicochemical parameters were almost stable throughout the study periods. The gills and water counts were rel¬atively lower than those of the intestine and sediment. Pearson’s correlation analysis established no significant correlations between the physicochemical parameters and viable bacterial counts. However, significant positive correlations were detected between the viable counts of water and sediment and between the gill and intestine. The RDA plot showed that, except in spring, the viable counts of a particular sample type were similar among the four locations. The results of permutation test showed that, individually none of the studied physicochemical parameters was significant; however, the seasons significantly affected the viable counts.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Viable bacterial counts of the Pangasius catfish (Pangasianodon hypophthalmus), their responses to seasonal variations of physicochemical parameters, and bacterial counts of the cultured ponds

Haider¹,

Islam²,

Mukit³

et al. 2022

J Adv Vet Anim Res

View full text Add to dashboard Cite

show abstract

“…The double-stranded DNAs were extracted from the preserved Sterivex-GP pressure filter units by Invitrogen Charge Switch Forensic DNA Purification Kits (Thermo Fisher Scientific, Waltham, MA, USA) with a slight modification adding bead-beating using FastGene ZircoPrep Mini (Nippon Genetics Co. Ltd., Bunkyo-Ku, Tokyo, Japan). The bead-beating cell breakage was achieved using a Micro Smash (MS-100R, Tomy Medico., Ltd., Tokyo, Japan) at 5000 rpm and 4 °C for 30 s 27 . The extracted DNAs were then stored at − 20 °C until polymerase chain reaction (PCR) amplification.…”

Section: Methodsmentioning

confidence: 99%

Bacterial response to glucose addition: growth and community structure in seawater microcosms from North Pacific Ocean

Haider

Iqbal

Nishimura

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Onboard microcosm experiments were conducted to assess how bacterial growth pattern and community structure changed by the addition of labile organic compound during the KH-14-2 cruise of R/V Hakuho Maru (Atmosphere and Ocean Research Institute, the University of Tokyo and JAMSTEC) in May–June 2014. Seawater samples were collected from the three diversified oceanic environments, Kuroshio Current, North Pacific Sub-polar Gyre (SPG), and North Pacific Sub-tropical Gyre (STG) in the western North Pacific Ocean, filtered, supplemented with glucose, and incubated at 23 ± 1 °C, ~ 4 °C, and 23 ± 1 °C, respectively. Untreated control microcosms were also maintained for all the sample types. Significant increases in cell counts and cell sizes were observed in Kuroshio Current and STG waters, whereas in SPG neither the counts nor the sizes changed, even after 120 h of incubation. At early stages of incubation, the classes Bacteroidia, Alphaproteobacteria, and Gammaproteobacteria were dominant in the Kuroshio Current and SPG samples, while the phyla Cyanobacteria and Proteobacteria in the STG samples. Over incubation periods between 60 and 96 h, some members of the class Gammaproteobacteria gradually dominated within which the genera Vibrio and Alteromonas became dominant in the Kuroshio Current and STG, respectively. No growth was detected for the microcosms with seawater from SPG, regardless of glucose amendment. It is concluded that depending on the environmental condition, certain different bacterial groups proliferated quickly and modified the community structures. Temperature significantly influenced the growth and succession, and ultimately the community structure of bacteria.

show abstract