Bacterial community shifts during healing of palatal wounds: comparison of two graft harvesting approaches

Delima, Suzanne L.; Kumar, Purnima; Tatakis, Dimitris N.

doi:10.1111/jcpe.12494

Cited by 4 publications

(8 citation statements)

References 37 publications

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“…As previously reported (Delima et al. ), 10 participants (mean age of 26.4 ± 1.7 years) complied with and completed the study protocol. Four participants had the right side of the palate pre‐wounded and six had the left side pre‐wounded.…”

Section: Resultsmentioning

confidence: 82%

“…The design and clinical aspects of this prospective, split‐mouth experimental study have been previously described in detail (Delima et al. ). Briefly, 12 periodontally and systemically healthy non‐smoking adult volunteers were recruited.…”

Section: Methodsmentioning

confidence: 99%

“…, Delima et al. ) studies, with few biochemical, immunohistochemical, and gene expression studies reporting on targeted specific molecular aspects of early gingival wound healing in humans (Green et al. , Warburton et al.…”

mentioning

confidence: 99%

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Human gingiva transcriptome during wound healing

Wang

Tatakis

2017

J Clinic Periodontology

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Section: Resultsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

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Human gingiva transcriptome during wound healing

Wang

Tatakis

2017

J Clinic Periodontology

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“…The design and clinical aspects of this prospective, split‐mouth experimental study have been previously described in detail 9,20 . Briefly, palatal tissues were obtained from nine periodontally and systemically healthy non‐smoking volunteers.…”

Section: Methodsmentioning

confidence: 99%

“…Biopsies were obtained solely for experimental purposes and were standardized in size, using metal templates, to control mesio‐distal (12 mm) and apico‐coronal (8 mm) dimensions; graft thickness was 1.25 to 1.5 mm 21 . One biopsy was from normal palatal gingiva, whereas the contralateral biopsy was from a matching area wounded 5 days earlier, 9,20 thus representing 5‐day healing gingiva. Harvested tissues were immediately sectioned in two halves, with one half stored in commercial solution for gene expression analysis and the other stored (10% neutral buffered formalin) for histology and immunohistochemistry.…”

Section: Methodsmentioning

confidence: 99%

Integrative mRNA/miRNA expression analysis in healing human gingiva

Wang

Tatakis

2020

Journal of Periodontology

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Background MicroRNAs (miRNAs) are implicated in the epigenetic regulation of complex biological processes. Their possible role in human oral wound healing, a process that differs from cutaneous wound healing by being faster and typically scar‐free, has been unexplored. This report presents the miRNA expression profile of experimental human oral wounds and an integrative analysis of mRNA/miRNA expression. Methods Nine healthy volunteers provided standardized normal and 5‐day healing palatal biopsies, used for next generation miRNA and mRNA sequencing analysis, correlation and network analysis, real‐time PCR (qPCR) and immunohistochemistry. Results On average, 169 significantly regulated precursor miRNAs were detected, including 21 novel miRNAs, selectively confirmed by PCR. Hsa‐miR‐223‐3p and hsa‐miR‐124‐3p were, respectively, the most up‐ and downregulated miRNAs in healing gingiva. Hsa‐miR‐124‐3p had the most predicted mRNA target interactions, with angiogenesis‐related genes the most enriched. Correlation analysis showed the highest correlation between hsa‐miR‐181a‐3p and SERPINB1; hsa‐miR‐223‐5p and SLC2A3; hsa‐miR‐1301 and MS4A7. In addition, SERPINB1 mRNA had the most associations with differentially regulated miRNAs. IL33 was the only cytokine significantly correlated with miRNAs (ρ > 0.95). qPCR and immunohistochemistry verified the significant upregulation of SERPINB1 and IL33 in healing gingiva. Conclusions This study is the first to report on the miRNome of healing human gingiva and to provide an integrative analysis of miRNA/mRNA expression during human oral wound healing; the results offer novel insights into the participating molecular mechanisms and raise the possibility of SERPINB1 and IL‐33 as potential wound healing therapeutic targets.

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Wound healing dynamics, morbidity, and complications of palatal soft‐tissue harvesting

Tavelli

Barootchi

Stefanini

et al. 2022

Periodontology 2000

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Palatal‐tissue harvesting is a routinely performed procedure in periodontal and peri‐implant plastic surgery. Over the years, several surgical approaches have been attempted with the aim of obtaining autogenous soft‐tissue grafts while minimizing patient morbidity, which is considered the most common drawback of palatal harvesting. At the same time, treatment errors during the procedure may increase not only postoperative discomfort or pain but also the risk of developing other complications, such as injury to the greater palatine artery, prolonged bleeding, wound/flap sloughing, necrosis, infection, and inadequate graft size or quality. This chapter described treatment errors and complications of palatal harvesting techniques, together with approaches for reducing patient morbidity and accelerating donor site wound healing. The role of biologic agents, photobiomodulation therapy, local and systemic factors, and genes implicated in palatal wound healing are also discussed.

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Bacterial community shifts during healing of palatal wounds: comparison of two graft harvesting approaches

Abstract: Pre-wounding FGG donor sites might accelerate the healing course and wounding the palate was associated with significant bacterial community shifts.

Cited by 4 publications

References 37 publications

Human gingiva transcriptome during wound healing

Human gingiva transcriptome during wound healing

Integrative mRNA/miRNA expression analysis in healing human gingiva

Wound healing dynamics, morbidity, and complications of palatal soft‐tissue harvesting

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