Bacterial Community Response to Petroleum Hydrocarbon Amendments in Freshwater, Marine, and Hypersaline Water-Containing Microcosms

Jurelevicius, Diogo; Alvarez, Vanessa Marques; Marques, Joana Montezano; Lima, Laryssa Ribeiro Fonseca de Sousa; Dias, Felipe de Almeida; Seldin, Lucy

doi:10.1128/aem.02251-13

Cited by 88 publications

(45 citation statements)

References 43 publications

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“…Marinobacter aquaeolei, Marinobacter maritimus and Marinobacter algicola) have been associated with aromatic and aliphatic hydrocarbon degradation [1,2,[9][10][11]. This may indicate a functional role in the organic carbon cycle, as they constitute one of the dominant bacterial community groups in contaminated environments [12]. The objectives of this study were to classify two newly isolated marine bacteria obtained when studying the diversity of halophilic bacteria associated with the marine environment.…”

mentioning

confidence: 99%

Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment

Rani

Koh

Kim³

et al. 2017

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, aerobic, motile, halophilic, rod-shaped bacteria, designated Hb8 T and Hb20, were isolated from a tidal flat environment located on the South-West Korean peninsula. The isolates grew at 10-37 C, at pH 5.0-9.0 and in NaCl concentrations of 0.5-15 % (w/v; optimum, 3.0-6.0 %). Sequence analysis of the 16S rRNA indicated that the isolates belong to the genus Marinobacter and are most closely related to Marinobacter sediminum R65 T (98.3 %), followed by Marinobacter lipolyticus SM19 T , Marinobacter salsuginis SD-14B T and Marinobacter similis A3d10 T . The overall 16S rRNA gene sequence similarity with these species was 97.9 %, but Hb8 T and Hb20 showed 100 % sequence similarity with each other. DNA-DNA relatedness values of H8 T and Hb20 suggested that these isolates represent a single species, while DNA-DNA relatedness values of the two novel isolates with M. sediminum DSM 27079 T and M. similis DSM 15400 T were only 21.3 and 22.9 %, respectively. The major fatty acids present in strain Hb8 T were identified as C 16 : 0 , C 16 : 1 !9c, C 18 : 1 !9c, C 18 : 0 3-OH and summed feature 3 (C 16 : 1 !6c and/or C 16 : 1 !7c). Ubiquinone-9 was the main respiratory quinone in both the novel strains. The polar lipids found to be present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids and five unidentified lipids. The genomic DNA G+C content of Hb8 T and Hb20 was 54.5 mol%. Polyphasic analysis indicated that the two isolates are representatives of a novel species of the genus Marinobacte, for which the name Marinobacter salinus sp. nov. is proposed. The type strain is Hb8 T (=KCTC 52255 T =JCM 31416 T ).The genus Marinobacter belongs to the family Alteromonadaceae, of the order Alteromonadales, class Gammaproteobacteria, and was first described by Gauthier et al.[1] to accomodate a hydrocarbon-degrading bacterium. At the time of writng, the genus Marinobacter comprises 40 species with validly published names (www.bacterio.net) isolated from a diverse range of environments including seawater [2,3], marine sand [4], marine sediment [5], the brine-seawater interface [6], coastal hot springs, saline soil [7], wastewater from wine production [8] and even laboratory cultures from dinoflagellates [9]. Members of the genus Marinobacter are Gram-stain-negative, oxidase-and catalase-positive, halophilic and rod-shaped [1]. Several species of the genus Marinobacter (i.e. Marinobacter aquaeolei, Marinobacter maritimus and Marinobacter algicola) have been associated with aromatic and aliphatic hydrocarbon degradation [1,2,[9][10][11]. This may indicate a functional role in the organic carbon cycle, as they constitute one of the dominant bacterial community groups in contaminated environments [12]. The objectives of this study were to classify two newly isolated marine bacteria obtained when studying the diversity of halophilic bacteria associated with the marine environment.Strains Hb8 T and Hb20 were isolated from tidal flat sediment sample...

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mentioning

confidence: 99%

Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment

Rani

Koh

Kim³

et al. 2017

International Journal of Systematic and Evolutionary Microbiology

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“…A dominant benzo (a) pyreme degrading bacteria obtained from a marine enrichment which contained three strains Cochrabactrum, Stenotrophomonas and Pseudomonas) degraded PAHs faster than when tested individually [46]. Pelz reported the biodegradation of 4-chlorosalicylate by combination of Pseudomonas MT1 [47], Pseudomonas MT4 and Achromobacter MT 4 using a 13 C-labelled substrates. Strain MT 1 degraded 4-chlorosalicylate and provided carbon seletons for the other strains which degraded the toxic metabolites that could have inhibited MT1 if it was allowed to accumulate.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies on PAHs degradation in marine environments implicated Cycloclasticus as a major PAH degrader during oil spills [11][12][13][14][15][16]. Marinobacter has also been reported to have degraded alkane and PAH whereas Flavobacterium was implicated PAH degradation [17,18].…”

Section: Introductionmentioning

confidence: 99%

Time Dependent Influence of Aerobic Heterotrophic Bacteria Cyanobacteria Interaction during Biodegradation of Poly Aromatic Hydrocarbons

Tersagh¹,

GM²

2016

J Pet Environ Biotechnol

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Biodegradation of polyaromatic hydrocarbons (PAHs) by aerobic heterotrophic bacteria, cyanobacteria, consortium of cyanobacteria and aerobic heterotrophic bacteria and the control was monitored for a period of 49 days, using GC-MS. The chromatograms showed that after contamination of water with same volume of crude in same quantity of water for all the treatments, PAHs were; 3.68, 23.5, 13.7 and 5.49 mg/L for AHB, CB, AHB + CB and control. The PAHs components were naphthalene, acencephthylene, acenaphthene, fluorence, anthracence, phenanthrene, fluoranthene, pyrene, beiz @ anthracene, chrysene, benzo (h) fluoranthene, benzo (k) fluoranthene, benzo (a) pyrene, dibenzo (a, h) anthracene, indeno (1, 2, 3-c, d) perylene, benzo (g, h, i) perylene. The result showed a decrease in the quantity of PAHs for all the treatment options on the last day of the experiment though some fluctuations in the quantity of PAHs' were observed in all the treatment options throughout the period monitored. The growth of AHB and cyanobacteria however increased steadily with time throughout the sampling period of the experiment in all the treatment options. The degradation of PAH's was statistically significant (p<0.05) with time.

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“…Acinetobacter strains are among the most abundant and ubiquitous microorganisms in soils and wastewater [34][35][36][37][38][39]. Notably, Acinetobacter strains are ubiquitous in oil-contaminated environments [40][41][42]. Acinetobacter isolates are generally equipped with an array of enzymes and degrading capabilities towards metabolizing alkanes and aromatic hydrocarbons [43][44][45].…”

Section: Removal Of Tph During Bioremediationmentioning

confidence: 99%

Assessing Technical and Economic Feasibility of Complete Bioremediation for Soils Chronically Polluted with Petroleum Hydrocarbons

Orellana¹,

Cumsille²,

Rojas³

et al. 2017

J Bioremediat Biodegrad

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Petroleum hydrocarbons are highly persistent in the environment and represent a significant risk for humans, biodiversity, and the ecosystems. Frequently, hydrocarbon-contaminated sites remain polluted for decades due to a lack of proper decontamination treatments. Although bioremediation techniques have gained attention for being environmentally friendly, cost-effective and applicable in situ, their application is still limited. Each polluted soil has particularities, therefore, the bioremediation approach for a contaminated site is unique. Bioremediation cost studies are usually based on hypothetical assumptions rather than technical or experimental data. The research aims of this study were to clean-up chronically hydrocarbon-polluted soils using aerobic and anaerobic bioremediation techniques and to carry out an economic evaluation of the most promising bioremediation treatments. The results showed that aerobic biostimulation with vermicompost and aerobic bioaugmentation plus air venting were the most effective treatments, degrading 78% and 73% of total petroleum hydrocarbons (TPH) in chronically hydrocarbonpolluted soils after six weeks, respectively. In contrast, no significant degradation of hydrocarbon was observed by anaerobic biostimulation treatments with lactate and acetate. An economic evaluation of the aerobic treatments were carried out. This analysis revealed that the cost of treating one cubic meter of soil by biostimulation is US$ 59, while bioaugmentation costs US$77. This study provides a clear structure of costs for both aerobic bioremediation approaches based on projections made from these lab-scale incubations. These values represent the first step towards a better understanding of the feasibility of such treatments at larger scales, which is crucial to move on industrial bioremediation of soils chronically polluted with petroleum hydrocarbons.

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Bacterial Community Response to Petroleum Hydrocarbon Amendments in Freshwater, Marine, and Hypersaline Water-Containing Microcosms

Cited by 88 publications

References 43 publications

Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment

Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment

Time Dependent Influence of Aerobic Heterotrophic Bacteria Cyanobacteria Interaction during Biodegradation of Poly Aromatic Hydrocarbons

Assessing Technical and Economic Feasibility of Complete Bioremediation for Soils Chronically Polluted with Petroleum Hydrocarbons

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