Bacterial community dynamics during the early stages of biofilm formation in a chlorinated experimental drinking water distribution system: implications for drinking water discolouration

Douterelo, Isabel; Sharpe, Rebecca; Boxall, Joby

doi:10.1111/jam.12516

Cited by 92 publications

(56 citation statements)

References 57 publications

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“…Thus, a relative abundance of a strain at 1% is considered to be a low abundance in a mixed population. Also, Methylobacterium species have been found to be present in drinking water in the United Kingdom [46,47] but they have not been found to be abundant in Glasgow tap water [40]. Here, only the case in which Methylobacterium was inoculated at the lowest relative abundance of 1% is reported.…”

Section: Inoculation Of Methylobacterium Into Drinking Watermentioning

confidence: 81%

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Tsagkari

Keating

Couto

et al. 2017

Water

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Abstract:The structure of biofilms in drinking water systems is influenced by the interplay between biological and physical processes. Bacterial aggregates in bulk fluid are important in seeding biofilm formation on surfaces. In simple pure and co-cultures, certain bacteria, including Methylobacterium, are implicated in the formation of aggregates. However, it is unclear whether they help to form aggregates in complex mixed bacterial communities. Furthermore, different flow regimes could affect the formation and destination of aggregates. In this study, real drinking water mixed microbial communities were inoculated with the Methylobacterium strain DSM 18358. The propensity of Methylobacterium to promote aggregation was monitored under both stagnant and flow conditions. Under stagnant conditions, Methylobacterium enhanced bacterial aggregation even when it was inoculated in drinking water at 1% relative abundance. Laminar and turbulent flows were developed in a rotating annular reactor. Methylobacterium was found to promote a higher degree of aggregation in turbulent than laminar flow. Finally, fluorescence in situ hybridisation images revealed that Methylobacterium aggregates had distinct spatial structures under the different flow conditions. Overall, Methylobacterium was found to be a key strain in the formation of aggregates in bulk water and subsequently in the formation of biofilms on surfaces.

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Section: Inoculation Of Methylobacterium Into Drinking Watermentioning

confidence: 81%

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Tsagkari

Keating

Couto

et al. 2017

Water

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“…The formation and growth of biofilm may be affected by factors such as temperature, pH, nutrient availability on the substrate, or time (Else et al 2003; Moghadam and Zimmer 2014; Rinaudi et al 2006). The growth of biofilm is characterized by an initial rapid proliferation of microbial cells and increased microbial richness and diversity, which finally leads to the formation of a stable climax community (Davey and O’toole 2000; Douterelo et al 2014). Microbial colonization of plant material also affects detritivore performance.…”

Section: Introductionmentioning

confidence: 99%

Biofilm feeding: Microbial colonization of food promotes the growth of a detritivorous arthropod

Horváthová¹,

Babik²,

Bauchinger³

2016

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Feeding on plant material is common among animals, but how different animals overcome the dietary deficiencies imposed by this feeding strategy is not well understood. Microorganisms are generally considered to play a vital role in the nutritional ecology of plant feeding animals. Commonly microbes living inside animal bodies are considered more important, but recent studies suggest external microbes significantly shape plant-feeding strategies in invertebrates. Here we investigate how external microbes that typically form biofilm on primary plant material affect growth rates in a terrestrial isopod species Porcellio scaber. We experimentally manipulated the amount of biofilm on three different primary diet sources and quantified growth and survival of individuals that fed on food with either a small or large amount of biofilm. In addition, we tested how dietary manipulation shapes the composition of bacterial communities in the gut. The presence of visible biofilm significantly affected the growth of isopods: individuals that fed on the primary diet source with a large amount of biofilm gained more mass than individuals feeding on a diet with marginal biofilm. Diet also significantly affected the bacterial gut community. The primary diet source mainly determined the taxonomic composition of the bacterial community in the isopod gut, whereas the amount of biofilm affected the relative abundance of bacterial taxa. Our study suggests that terrestrial isopods may cope with low-quality plant matter by feeding on biofilm, with decomposition of plant material by organisms outside of the feeding organism (here a terrestrial isopod) probably playing a major role. Future investigations may be directed towards the primary diet source, plant matter, and the secondary diet source, biofilm, and should assess if both components are indeed uptaken in detritivorous species.

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“…A notable exception is the study by Salehi and colleagues 34 , where they monitored changes in water chemistry and bacterial growth during the first days/weeks of building occupation. Also, a study by Douterelo and colleagues 47 , showed that specific bacteria are dominant during the initial colonization (7 – 28 d) of distribution pipe materials. The fact is, in current practice the owners/operators have effectively no control over the communities that colonize their building plumbing systems.…”

Section: Discussionmentioning

confidence: 99%

Towards a probiotic approach for building plumbing – nutrient-based selection during initial biofilm formation on flexible polymeric materials

Neu

Laura

Hammes

2020

Preprint

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26Upon entering building plumbing systems, drinking water bacteria experience 27 considerable changes in environmental conditions. For example, some flexible 28 polymeric materials leach organic carbon, which increases bacterial growth and 29 reduces diversity. Here we show that the carbon supply by a flexible polymeric material 30 drives nutrient-based selection within establishing biofilm communities. We found that 31 migrating carbon from EPDM coupons resulted in considerable growth for different 32 drinking water communities (0.2 -3.3 x 10 8 cells/cm 2 ). All established biofilm 33 communities showed low diversity (29 -50 taxa/biofilm), with communities dominated 34 by even viewer taxa (e.g., 5 taxa accounting for 94 ± 5 % relative abundance, n = 15). 35Interestingly, biofilm communities shared some taxa (e.g., Methylobacterium spp.) and 36 families (e.g., Comamonadaceae), despite the difference in starting communities. 37Moreover, selected biofilm communities performed better than their original 38 communities regarding maximum attachment (91 ± 5 vs. 69 ± 23 %, n = 15) and 39 attachment rate (5.0 ± 1.7 x 10 4 vs. 2.4 ± 1.2 x 10 4 cells/cm 2 /h, n = 15) when exposed 40 to new EPDM coupons. Our results demonstrate nutrient-based selection during initial 41 biofilm formation on a flexible polymeric material and a resulting benefit to selected 42 communities. We anticipate our findings to help connecting observational 43 microbiological findings with their underlying ecological principles. Regarding initial 44 biofilm formation, attachment dynamics, growth, and selection thereof are important 45 for the management of microbial communities. In fact, managing initial colonization by 46 supplying specific carbon and/or introducing consciously chosen/designed 47 communities potentially paves the way for a probiotic approach for building plumbing 48 materials. 49 50

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Bacterial community dynamics during the early stages of biofilm formation in a chlorinated experimental drinking water distribution system: implications for drinking water discolouration

Cited by 92 publications

References 57 publications

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Biofilm feeding: Microbial colonization of food promotes the growth of a detritivorous arthropod

Towards a probiotic approach for building plumbing – nutrient-based selection during initial biofilm formation on flexible polymeric materials

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