Bacterial community diversity in a full scale biofilter treating wastewater odor

Abstract

Constantly, the odors coming from sewage plants are considered a problem by the population. The purpose of this study was to evaluate the microbial community present in a full scale biofilter used for odor treatment. The filter was packed with peat. The main gas treated was hydrogen sulphide (HS). The removal efficiency reached 99%, with an empty bed residence time of 30 seconds. Molecular analysis can enhance our understanding of the microbial communities in biofilters treating wastewater odor. The analysis made to characterize microbial community was High-throughput 16S rRNA sequencing analysis MiSeq Illumina. The sampling, carried out in the year 2015, was seasonal (summer and winter) and spatial (depth and position in the biofilter). In this study, a total of 206,174 raw sequence reads for six samples were analyzed using Mothur software (v 1.33.3) based on MiSeq SOP protocol. After Mothur analysis, the results of the bacterial community were explored at the Phylum and Genus levels. In this study, the efficiency removal of hydrogen sulfide reached values greater than 99% during the monitoring, and the main bacterial genera found were Acidotermus, Telmatobacter, Methylovirgula and Bryobacter representing the bacterial community active in the transformation of HS into a system with long operating time.

Insights into the antibiotic resistance dissemination in a wastewater effluent microbiome: bacteria, viruses and vesicles matter

“…Data showed that the predominant phyla in control and treated samples were Proteobacteria, Saccharibacteria and Bacteroidetes. Furthermore, candidate phyla usually found in wastewater, such as WCHB1-60 and TM6 (Tian et al, 2015;Yeoh et al, 2016;Allievi et al, 2018), were also detected in samples. At the genus level, Aquicella, uncultured Saccharibacteria and Flavobacterium dominated the community in both control and treated antibiotic samples ( Fig.…”

Low-dosage Ozonation in Gas-phase Biofilter Promotes Community Diversity and Robustness.

“…Correlation analysis of performance and microbes showed that the commonly seen genus Rhodococcus negatively correlated with performance after ozonation. As numerous studies have reported that this genus is dominant in the degradation of xenobiotics in bio lter systems (Portune et al, 2015, Allievi et al, 2018, this indicated a shift in degrading contribution away from Rhodococcus and more toward ASVs from the genera Devosia, Aquamicrobium, and Rhizobiales, which had high positive correlation with performance. The genus Pandoreae was reported to be highly enriched in species capable of effective degradation of xenobiotics (Peeters et al, 2019), and was positively correlated with r>0.7 in both bio lters as shown in Fig.…”

“…Mesorhizobium is an genus containing species strongly correlated to nitrogen xation and denitri cation with aerobic respiration, yet the genus was uncommonly reported to be associated with gaseous bio lters, correlation analysis of Mesorhizobium's relative abundance and performance as seen in Fig.5a shows that the genus strongly correlates with mineralization rate of the bio lter after ozonation, this could be explained by enhanced effective use of nitrogen sources through nutrient spraying (Delgado et al, 2007). Proteobacteria in general has been widely reported to contain the dominant degraders of xenobiotics in both bio lter and wastewater treatments (Allievi et al, 2018, Van der Heyden et al, 2019. Proteobacteria has also been reported to have a higher presence in the treatment of more complex pollutants and higher loading stress, inducing higher functional diversity (Friedrich and Lipski 2010).…”

Low-Dosage Ozonation in Gaseous VOCs Biofilter Promotes Community Diversity and Robustness

“…After ozonation, Proteobacteria increaed in relative abundance, which was widely reported to contain dominant degrader of xenobiotics in both bio lter and wastewater treatments (Allievi, Silveira et al 2018, Van der Heyden, De Mulder et al 2019), reported for higher presence in treatment of more complex pollutant and higher loading stress, inducing higher functional diversity (Friedrich and Lipski 2010). In contrast, this could be a phenotypic embodiment of larger community diversity seen in sequencing results, the ability to better metabolize carbon sources other than the only provided toluene, while degradation of xenobiotics such as toluene had highly diverse prokaryotic pathways, a more even and less specialized phenotypic functional pro le may be desirable in full-scale applications when composition of pollutants is largely uctuated, though this decrease of complex carbohydrate degradation and increases in other compounds caused by ozonation did not impinge removal e ciency of bio lter.…”

“…9 out of 20 genera are monotonously correlated with removal e ciency in both ozone and control bio lter, whereas only 3 out of 20 OTUs are monotonously correlated with removal e ciency, indicating a high speci city and different reaction of OTUs under same genus towards ozonation. genus Rhodococcus was reported in numerous studies to be dominant in degrdation of xenobiotics in bio lter systems (Portune, Perez et al 2015, Allievi, Silveira et al 2018, all four OTUs from the genus Rhodococcus present in this system are strongly proportional with removal e ciency in control bio lter but all negatively correlates in ozonated bio lter, suggesting a shift of degrading contribution by Rhodococcus and more towards OTUs of genera Devosia, Aquamicrobium and Rhizobiales with high positive correlation with performance, similar trends of these three genus are seen in combined genus level.…”