Bacterial Community Composition of Throughfall and Stemflow

Teachey, Morgan E.; Pound, Preston; Ottesen, Elizabeth A.; Stan, John T. Van

doi:10.3389/ffgc.2018.00007

Cited by 15 publications

(12 citation statements)

References 35 publications

(39 reference statements)

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“…3 ) was enriched within throughfall compared to pure rainwater via increased nitrifying microbial activity 61 . Despite limitations in interpreting results from previous studies that have not explicitly accounted for the compositional nature of microbial community data 60,103 which can introduce bias, our results of relative patterns of taxa found in throughfall and rainwater support previous findings. In a previous study, microbial communities also differed between rainwater and throughfall samples, and we detected 14 of 15 (93%) of the same bacterial phyla (Fig.…”

Section: Discussionsupporting

confidence: 82%

“…6 ) within our samples that as those detected within their study 61 . Despite not being able to make direct comparisons about community diversity per se , in general, we detected 82% of the same classes found in another study that explored how variability in storm events can influence microbial community inputs into forests as well as increasing the transfer of bacteria from the canopy to the forest floor via throughfall and stemflow 60 .…”

Section: Discussionmentioning

confidence: 57%

“…It has also been well established that airborne biological particles occur within the atmosphere 56 , and several studies have been successful in sampling and sequencing eDNA from airborne samples 46,[57][58][59] . However, very few studies to date have explored the partitioning of microbial communities contained and transported in pure rainwater and throughfall 60,61 . Throughfall is the incident precipitation that passes either directly through the forest canopy, or alternatively is first intercepted and splashed from the canopy or coalesces and drips down to lower strata 62,63 .…”

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confidence: 99%

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Assessing the efficacy of eDNA metabarcoding for measuring microbial biodiversity within forest ecosystems

Ladin

Ferrell

Dums

et al. 2021

Sci Rep

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We investigated the nascent application and efficacy of sampling and sequencing environmental DNA (eDNA) in terrestrial environments using rainwater that filters through the forest canopy and understory vegetation (i.e., throughfall). We demonstrate the utility and potential of this method for measuring microbial communities and forest biodiversity. We collected pure rainwater (open sky) and throughfall, successfully extracted DNA, and generated over 5000 unique amplicon sequence variants. We found that several taxa including Mycoplasma sp., Spirosoma sp., Roseomonas sp., and Lactococcus sp. were present only in throughfall samples. Spiroplasma sp., Methylobacterium sp., Massilia sp., Pantoea sp., and Sphingomonas sp. were found in both types of samples, but more abundantly in throughfall than in rainwater. Throughfall samples contained Gammaproteobacteria that have been previously found to be plant-associated, and may contribute to important functional roles. We illustrate how this novel method can be used for measuring microbial biodiversity in forest ecosystems, foreshadowing the utility for quantifying both prokaryotic and eukaryotic lifeforms. Leveraging these methods will enhance our ability to detect extant species, describe new species, and improve our overall understanding of ecological community dynamics in forest ecosystems.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 57%

mentioning

confidence: 99%

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Assessing the efficacy of eDNA metabarcoding for measuring microbial biodiversity within forest ecosystems

Ladin

Ferrell

Dums

et al. 2021

Sci Rep

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“…Throughfall is enriched with nutrients that accumulate in forest canopies during antecedent dry periods which are subsequently washed off, and also leached from plant surfaces during rainfall [48][49][50]. Species-level differences in throughfall have been observed across a range of forest functional types [51][52][53], and recent work has linked canopy-derived nutrient inputs with soil microbiological communities [54][55][56][57]. For example, presence of canopy epiphytes increased nutrient fluxes in throughfall and led to differences in abundance of ammonia-oxidizing soil bacteria, compared to soils underneath trees where epiphytes were not present [54].…”

Section: Discussionmentioning

confidence: 99%

Spatial and Temporal Variability of Throughfall among Oak and Co-Occurring Non-Oak Tree Species in an Upland Hardwood Forest

2019

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Canopy throughfall comprises the largest portion of net precipitation that is delivered to the forest floor. This water flux is highly variable across space and time and is influenced by species composition, canopy foliage, stand structure, and storm meteorological characteristics. In upland forests throughout the central hardwoods region of the Eastern United States, a compositional shift is occurring from oak-hickory to more mesic, shade-tolerant species such as red maple, sweetgum, and winged elm. To better understand the impacts of this shift on throughfall flux and the hydrologic budget, we monitored throughfall for one year in Northern Mississippi under the crowns of midstory and overstory oak (post oak and southern red oak) and non-oak species (hickory, red maple, and winged elm). In general, oak had more throughfall than co-occurring non-oak species in both canopy levels. In the overstory during the leaf-off canopy phase, white oak had relatively higher throughfall partitioning (standardized z-score = 0.54) compared to all other species (z-score = −0.02) (p = 0.004), while in the leaf-on canopy phase, red maple had relatively lower throughfall (z-score = −0.36) partitioning compared to all other species (z-score = 0.11). In the midstory, red maple was the only species to exhibit a difference in throughfall between canopy phases, with much lower throughfall in the leaf-off compared to the leaf-on canopy phase (z-score = −0.30 vs. 0.202, p = 0.039). Additionally, throughfall under oak crowns was less variable than under non-oak crowns. These results provide evidence that the spatial and temporal distribution of throughfall inputs under oak crowns are different than non-oak species, likely due to differences in crown architecture (i.e., depth and density). As oak dominance diminishes in these forests, it is possible that the portion of rainfall diverted to throughfall may decrease as well. The net impacts to watershed hydrology are still unknown, but these results provide one mechanism by which the distribution of water resources may be affected.

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“…The mobility of some phyllosphere bacterial taxa in hydrologic highways may be a function of ecological interactions, such that some bacteria gain entrance into protective aggregates or biofilms while others do not (Teachey et al . 2018).…”

Section: Do We Stay or Do We Go? Mobility Of Canopy Travelers And Vehiclesmentioning

confidence: 99%

Throughfall and stemflow are major hydrologic highways for particulate traffic through tree canopies

Stan

Ponette‐González

Swanson

et al. 2021

Frontiers in Ecol & Environ

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For one‐third of Earth’s land surface, precipitation passes through tree canopies (as throughfall or stemflow) before entering watersheds. Over a century of research has described fluxes of water and solutes along these “hydrologic highways”, yet little is known about their “traffic” – that is, the organisms and nonliving particulates frequently discarded from water samples after filtration in the lab. A comprehensive understanding of the composition of sub‐canopy precipitation is necessary to estimate the total nutrient and pollutant inputs to watersheds for redistribution downstream, as well as to systematically investigate precipitation effects on organismal exchanges along the atmosphere–plant–soil continuum. Here, we review current concepts and research showing that the hydrologic highways from tree canopies to soil carry ecologically relevant quantities of biologic (viruses, microbes, microfauna, and meiofauna) and abiotic particulates. Their fate may have important consequences for the biogeochemistry and biodiversity of terrestrial systems.

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Bacterial Community Composition of Throughfall and Stemflow

Cited by 15 publications

References 35 publications

Assessing the efficacy of eDNA metabarcoding for measuring microbial biodiversity within forest ecosystems

Assessing the efficacy of eDNA metabarcoding for measuring microbial biodiversity within forest ecosystems

Spatial and Temporal Variability of Throughfall among Oak and Co-Occurring Non-Oak Tree Species in an Upland Hardwood Forest

Throughfall and stemflow are major hydrologic highways for particulate traffic through tree canopies

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