Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations

Abstract

To understand how soil microbial communities and arsenic (As) functional genes respond to soil arsenic (As) contamination, five soils contaminated with As at different levels were collected from diverse geographic locations, incubated for 54 days under flooded conditions, and examined by both MiSeq sequencing of 16S rRNA gene amplicons and functional gene microarray (GeoChip 4.0). The results showed that both bacterial community structure and As functional gene structure differed among geographical locations. The diversity of As functional genes correlated positively with the diversity of 16S rRNA genes (P< 0.05). Higher diversities of As functional genes and 16S rRNA genes were observed in the soils with higher available As. Soil pH, phosphate-extractable As, and amorphous Fe content were the most important factors in shaping the bacterial community structure and As transformation functional genes. Geographic location was also important in controlling both the bacterial community and As transformation functional potential. These findings provide insights into the variation of As transformation functional genes in soils contaminated with different levels of As at different geographic locations, and the impact of environmental As contamination on the soil bacterial community.

Molecular Biology-Based Analysis of the Interactive Effect of Nickel and Xanthates on Soil Bacterial Community Diversity and Structure

“…As it is for Betaproteobacteria class, Gammaproteobacteria did not show a significant difference between the xanthates doses and their mixed-chemicals doses. The proportion of Proteobacteria in Ni-treated samples (15%) was lower than that recently observed in arsenic-contaminated soil where it was the dominant phylum (29-38% abundant), Alphaproteobacteria and Gammaproteobacteria being the most and least abundant classes [55]. The most abundant and proportionally different bacterial classes between the xanthate-treated samples and their corresponding mixtures-treated samples were presented, both in the case of 50 µg·g −1 soil ( Figure 5A) and in the case of 100 µg·g −1 soil ( Figure 5B).…”

Diversity of Betaproteobacteria revealed by novel primers suggests their role in arsenic cycling

“…Similar observations were reported by Hassan et al where abundance of Betaproteobacteria was observed as the most abundant class (~69% of the community) in a comparative examination of microbial communities from 24 groundwater samples in Bangladesh [17]. In addition to BDP aquifers, this bacterial class also has been shown to dominate the microbial communities in As-contaminated soils from China and the United Kingdom [18]. Taken together, these observations are indicative of an emerging pattern where members of Betaproteobacteria are major inhabitants of arsenic-contaminated subsurface in geographically distant regions.…”

Characteristics of Bacterial Community and Function in Paddy Soil Profile around Antimony Mine and Its Response to Antimony and Arsenic Contamination

“…Relative importance analysis showed that Chloroflexi was strongly influenced by Sbrec, Astot, and Asrec, while Proteobacteria was mainly influenced by Astot. According to previous reports, Anaerolineaceae, as a specific anaerobic of Chloroflexi, existed in various environmental media contaminated by Sb and As [57,58], and Anaerolineaceae has also been reported to be widely distributed in contaminated paddy soil in China, Britain, and Bangladesh [59]. We found that Anaerolineaceae was the most abundant family of Chloroflexi and it decreased with the deepening of soil layer.…”