Bacterial community analysis of anoxic/aeration (A/O) system in a combined process for gibberellin wastewater treatment

Abstract

Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological contact oxidation combined processes are used to treat gibberellin wastewater. However, knowledge of the treatment effects of the A/O process and bacterial community structure in the aeration tank reactors of such systems is sparse. Therefore, this study was conducted to investigate the treatment effects and operation of the A/O process on gibberellin wastewater, as well as changes in the bacterial community structure of activated sludge in the aeration tank during treatment. Moreover, removal was examined based on evaluation of effluent after A/O treatment. Although influent chemical oxygen demand (COD), NH3-N and total phosphorus (TP) fluctuated, effluent COD, NH3-N and TP remained stable. Moreover, average COD, NH3-N and TP removal efficiency were 68.41%, 93.67% and 45.82%, respectively, during the A/O process. At the phylum level, Proteobacteria was the dominant phylum in all samples, followed by Chloroflexi, Bacteroidetes and Actinobacteria. Proteobacteria played an important role in the removal of organic matter. Chloroflexi was found to be responsible for the degradation of carbohydrates and Bacteroidetes also had been found to be responsible for the degradation of complex organic matters. Actinobacteria are able to degrade a variety of environmental chemicals. Additionally, Anaerolineaceae_uncultured was the major genus in samples collected on May 25, 2015, while Novosphingobium and Nitrospira were dominant in most samples. Nitrosomonas are regarded as the dominant ammonia-oxidizing bacteria, while Nitrospira are the main nitrite-oxidizing bacteria. Bacterial community structure varied considerably with time, and a partial Mantel test showed a highly significant positive correlation between bacterial community structure and DO. The bacterial community structure was also positively correlated with temperature and SO42-.

Assessing bacterial diversity and antibiotic resistance dynamics in wastewater effluent-irrigated soil and vegetables in a microcosm setting

“…Actinobacteria consists of many Gram-negative bacteria that play an important role in carbon cycling and degrading environmental chemicals. The results of this study are supported by Ouyang et al. (2017) who previously identified the phyla as the most prevalent in activated sludge and wastewater treated soils.…”

16S rRNA Gene Amplicon Sequencing Data of the Iron Quadrangle Ferruginous Caves (Brazil) Shows the Importance of Conserving This Singular and Threatened Geosystem

“…Additionally, all these genera are directly or indirectly associated with nutrient cycling (e.g., recalcitrant organic compound and organic matter; Atashgahi et al [117]) maintenance of biogeochemical cycles (carbon; [118] and nitrogen; [119]), and other metabolic functions (fixation, reduction, and oxidation of metals). These organisms are therefore suitable for several biotechnological applications, such as rhizoremediation [120], effluent treatment [121], promotion of plant growth [120], plant pathogen control [122], and metal biotransformation [123]. In the floor samples, the genera Burkholderia, Rhodanobacter, and Variovorax dominated.…”

Effect of inoculated and uninoculated aeration pretreatment on nutrients and phytotoxicity of anaerobic digestion effluent

“…Aeration plays an important role for the biological degradation of organic matter in wastewater treatment 8 – 10 . Thus, aeration pretreatment can be potentially applied to condition the AD effluent by providing aerobic conditions for the oxidation of unfavorable organic acids 11 , 12 .…”