Bacterial communities related to 3-nitro-1-propionic acid degradation in the rumen of grazing ruminants in the Qinghai-Tibetan Plateau

Guo, Wei; Bi, Sisi; Kang, Jingpeng; Zhang, Ying; Long, Ruijun; Huang, Xiaodan; Shan, M.N.; Anderson, Robin C.

doi:10.1016/j.anaerobe.2018.07.013

Cited by 10 publications

(11 citation statements)

References 41 publications

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“…Alpha diversity metrics (Shannon, Chao1 and Observed_species) displayed a higher diversity in yak compared to Tibetan sheep and Small Tail Han sheep. Our previous study on rumen bacteria community using the same animals [19] as well as another study on grazing yaks and Tibetan sheep [38] found the similar result on alpha diversity indices, indicating that the animal body size or diet may have the similar effect on prokaryotic and eukaryotic microorganisms in rumen. Further studies could be done on rumen archaea and protozoa to warrant this hypothesis.…”

Section: Discussionsupporting

confidence: 61%

“…Yak and Tibetan sheep are indigenous animals in the Qinghai-Tibetan Plateau that is characterized by cold weather conditions and mountainous terrain with altitudes ranging from 4000 to 5500 m, which provide milk, meat, fuel (yak dung), and wool for local herdsmen [17]. Small Tail Han sheep, which originated from low land [18], later adapted to the grazing lifestyle on the Qinghai-Tibetan Plateau and became an indispensable species to the local life and economy [19]. Although a few studies have reported the rumen prokaryotic community from grazing yak, Tibetan sheep and Small Tail Han sheep [19][20][21][22][23], few studies have considered the rumen eukaryotic community composition of these three ruminant species, especially grazing under the same natural pasture.…”

Section: Introductionmentioning

confidence: 99%

“…Small Tail Han sheep, which originated from low land [18], later adapted to the grazing lifestyle on the Qinghai-Tibetan Plateau and became an indispensable species to the local life and economy [19]. Although a few studies have reported the rumen prokaryotic community from grazing yak, Tibetan sheep and Small Tail Han sheep [19][20][21][22][23], few studies have considered the rumen eukaryotic community composition of these three ruminant species, especially grazing under the same natural pasture. Thus, our prime aim of this study is to characterize the rumen fungal community of mainly grazing ruminant species (Yak, Tibetan sheep and Small Tail Han sheep) in the Qinghai-Tibetan Plateau, so as to identify the ruminal fungal strains that are prevalent in the rumen which may be associated with fiber degradation.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Anaerobic Rumen Fungal Community Composition in Yak, Tibetan Sheep and Small Tail Han Sheep Grazing on the Qinghai-Tibetan Plateau

Guo

Wang

et al. 2020

Animals

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The anaerobic rumen fungal community play a critical role in fibrous material degradation. However, there is a lack of data describing the composition of anaerobic rumen fungal community of full grazing ruminants in the Qinghai-Tibetan Plateau. For this reason, we employed the next-generation sequencing technique to elucidate the rumen fungal structure composition and evaluate the effects of host species on fungal communities. Community comparisons (Bray–Curtis index) between yak and Tibetan sheep revealed that the rumen fungal community was affected by host species (p < 0.05). The alpha diversity indices in the yak were significantly higher than in the Tibetan sheep and Small Tail Han sheep. Neocallimastigomycota was predominant regardless of host species. Within this phylum, unidentified genus of Neocallimastigaceae was the most dominant in all samples, followed by Piromyces and Orpinomyces. Moreover, the shared and unique OTUs in the rumen were identified and most of them belonged to the Orpinomyces. Co-occurrence network analysis identified that each animal species had their own keystone species and most of them were non-dominant flora. Our data indicate that host breeds override living environment as the key factor that determines fungal community in the rumen of grazing ruminants in the Qinghai-Tibetan Plateau.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Anaerobic Rumen Fungal Community Composition in Yak, Tibetan Sheep and Small Tail Han Sheep Grazing on the Qinghai-Tibetan Plateau

Guo

Wang

et al. 2020

Animals

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“…In a study on Yak and Tibetan sheep, it was shown that the prokaryotic community structure between yak and Tibetan sheep was significantly different (p < 0.01) [36]. In another study on yak rumen, a high bacterial diversity was observed when compared to Tibetan sheep [37]. These studies considered the host as the major influencing factor but did not consider the differences in feeding habits.…”

Section: Rumen Microbiota May Reflect Host's Adaptive Capacity Of Envmentioning

confidence: 99%

Diversity and Composition of Rumen Bacteria, Fungi, and Protozoa in Goats and Sheep Living in the Same High-Altitude Pasture

Suo

Zhang

et al. 2020

Animals

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Environmental adaptation of ruminants was highly related to microbiota in the rumen. To investigate the diversity and composition of bacteria, fungi, and protozoa in the rumen of high-altitude animals, amplicon gene sequencing was performed using rumen fluid samples derived from both Tibetan goats and sheep at the same pasture in a highland (altitude > 4800 m). Between these two species, the ruminal bacteria and fungi were significantly different at multiple taxonomic levels. The alpha diversity of bacteria was significantly high in goats (p < 0.05). One hundred and sixty-four and 29 Operational Taxonomy Units (OTUs) with significant differences were detected in bacteria and fungi, respectively. The abundance of bacteria, fungi, and protozoa in the rumen was characterized at multiple taxonomic levels, and we determined that Firmicutes, Bacteroidetes, Neocallimastigomycota, and Ciliophora were the most abundant bacteria, fungi, and protozoa. The family Neocallimastigaceae and the genus Metadinium had cellulose degradation capacity in the rumen with high abundance, thereby, suggesting that fungi and protozoa played an essential role in rumen fermentation. In addition, by comparing microbiota in the rumen of goats and sheep it was found, that the fiber-degrading fungi genus (Cyllamyces) was increased in the rumen of sheep (p < 0.05) whereas VFA-producing bacteria (Saccharofermentans and Lachnospiraceae_XPB1014) were increased in the rumen of goats (p < 0.05). Interestingly, in the rumen, no differences in protozoa were observed between goats and sheep (p > 0.05). Furthermore, when compared to sheep, level of acetic acid, propionic acid, and total volatile fatty acid (TVFA) were significantly increased in the rumen of goats (p < 0.05). Taken together, these results suggested microbiota in the rumen drive goats to better adapt to high-altitude grazing conditions.

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“…Diet and environmental conditions influence the microbial community composition; however, little is known about the bacterial community changes over time when ruminants are on similar diets, especially in the QTP where the forage differs considerably throughout a whole year due to the harshness of the environment. Some studies have been conducted on rumen microbiota in grazing yaks and sheep in QTP (Dan et al 2016;Huang et al 2017;Guo et al 2018). Changes in temperature and precipitation directly affect plant yak productivity, which in turn affects animal growth and the microbiome in the body.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai-Tibet Plateau (v0.1)"

2019

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The rumen microbiota of ruminants plays a vital role in fiber digestion, and environmental factors affect its community structure. The yak (Bos grunniens) is the main livestock species that inhabits the Qinghai-Tibet Plateau (QTP) at regions located at high-altitude of 3000-5000 m. This work investigated the rumen bacterial community of yak that grazed on the QTP during the whole year to evaluate the relationship between the rumen bacterial community and the nutrient composition of forage plant at three stages. In this study, the diversity of the rumen prokaryotic community composition was monitored in 10 fullgrazing yak in an alpine meadow of the QTP. The nutrient composition of three forage growth stages was determined: re-green stage (REGY), grassy stage (GY), and withered stage (WGY). High-throughput sequencing of bacterial 16S rRNA gene was used. The results showed that the nutritive composition of the alpine meadow changed with the seasons: crude protein (CP) (13.22%) was high in forage during REGY (spring), while neutral detergent fiber (NDF) (59.00%) was high during WGY (winter). Microbial diversity and richness were highest during REGY and the average number of operational taxonomic units from 30 samples was 4470. The microbial composition was dominated by members of Bacteroidetes (51.82%), followed by Firmicutes (34.08%), and the relative microbial abundance changed in the three forage growth stages. Unweighted UniFrac distance PcoA showed that the bacterial community structure differed between REGY, GY, and WGY. Furthermore, taxonomic groups did not present differences regarding gender in these three stages. The rumen microbiota was enriched with functional potentials that were related to ABC transporters, the Two component system, Aminoacyl-tRNA biosynthesis, and metabolism of Purine, Pyrimidine, Starch and sucrose metabolism. Significant differences were found in the composition, diversity, and function of yak ruminal microorganisms during different forage growth stages. This indicates that microbial changes in the rumen depend on changes in the forage nutritional composition. These

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Bacterial communities related to 3-nitro-1-propionic acid degradation in the rumen of grazing ruminants in the Qinghai-Tibetan Plateau

Cited by 10 publications

References 41 publications

Characterization of Anaerobic Rumen Fungal Community Composition in Yak, Tibetan Sheep and Small Tail Han Sheep Grazing on the Qinghai-Tibetan Plateau

Characterization of Anaerobic Rumen Fungal Community Composition in Yak, Tibetan Sheep and Small Tail Han Sheep Grazing on the Qinghai-Tibetan Plateau

Diversity and Composition of Rumen Bacteria, Fungi, and Protozoa in Goats and Sheep Living in the Same High-Altitude Pasture

Peer Review #2 of "Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai-Tibet Plateau (v0.1)"

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