Bacterial Communities in the Sediments of Dianchi Lake, a Partitioned Eutrophic Waterbody in China

Abstract

Bacteria play an important role in the decomposition and cycling of a variety of compounds in freshwater aquatic environments, particularly nutrient-rich eutrophic lakes. A unique Chinese eutrophic lake - Dianchi - was selected for study because it has two separate and distinct basins, Caohai with higher organic carbon levels and Waihai with lower organic carbon levels. Sediment bacterial communities were studied in the two basins using samples collected in each season from June 2010 to March 2011. Barcoded pyrosequencing based on the 16 S rRNA gene found that certain common phyla, Proteobacteria, Bacteroidetes, Firmicutes and Chloroflexi, were dominant in the sediments from both basins. However, from the class to genus level, the dominant bacterial groups found in the sediments were distinct between the two basins. Correlation analysis revealed that, among the environmental parameters examined, total organic carbon (TOC) accounted for the greatest proportion of variability in bacterial community. Interestingly, study results suggest that increasing allochthonous organic carbon could enhance bacterial diversity and biomass in the sediment. In addition, analysis of function genes (amoA and nosZ) demonstrated that ammonia-oxidizing bacteria (AOB) were dominant in sediments, with 99% belonging to Nitrosomonas. Denitrifying bacteria were comparatively diverse and were associated with some cultivatable bacteria.

Effect of sulfate on the transformation of corrosion scale composition and bacterial community in cast iron water distribution pipes

“…The 16S rRNA gene of DNA was amplified by PCR using barcoded primers 8F and 533R containing the A and B sequencing adaptors (454 Life Sciences, USA) (Bai et al, 2012). The fusion Primer A-8F was 5 0 -cgtatcgcctccctcgcgccatcagAGAGTTTGAT-CCTGGCTCAG-3 0 , where the sequence of the A adaptor is shown in lowercase letters.…”

“…Sequence reads were initially filtered and denoized for removing low quality or ambiguous reads (Bai et al, 2012). Then the treated sequences were subjected to systematic checks to remove replicates, duplicates, barcodes, primer sequences and low-quality reads.…”

Formation and release behavior of iron corrosion products under the influence of bacterial communities in a simulated water distribution system

“…20 PCR amplication was performed as described previously. 21 Positive PCR products were puried with an AxyPrep DNA Gel Extraction Kit (Axygen, USA).…”

Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river

“…WWTP represents wastewater treatment plant were amplified from triplicate water DNA samples by PCRs using barcoded primers modified with 5'Adaptor-A and 5'Adaptor-B sequences (454 Life Sciences), respectively. PCR primer pairs, thermal program, and barcode primers were described in supplemental Table S1 and a previous study (Bai et al 2012). The PCR products were then purified with AxyPrep DNA Gel Extraction Kit (Axygen, USA).…”

“…The primer pairs and the thermal programs of the PCR amplification are described in the Supplemental Table S1. Plasmid standards and calibration curves construction were described in detail previously (Bai et al 2012).…”